人Tau ELISA试剂盒

产品详情

Human Tau ELISA Kit ab273617 is a rapid single-wash 90-min ELISA kit to measure Human Tau in cerebral spinal fluid (CSF) cell culture extracts, tissue extracts, cell culture media, serum and plasma. This SimpleStep ELISA® sensitivity is 16 pg/mL.

How the assay works

Human Tau SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant human Tau protein in serum, plasma (EDTA and heparin), cell culture supernatant, cerebrospinal fluid, cell and tissue extract samples only. Urine, milk, saliva samples have not been tested with this kit. This kit is incompatible with plasma (citrate) samples.
The kit is 100% reactive with human recombinant Tau-A isoform and 61% reactive with human recombinant Tau-F isoform.
Recombinant human UCHL-1 and alpha synuclein were prepared at 7 ng/mL and assayed for cross reactivity. No cross-reactivity was observed.
Recombinant human UCHL-1 and alpha synuclein were prepared at 50 ng/mL and 2 ng/mL and tested for interference with 2 ng/mL of recombinant human Tau. No interference with was observed.

Human Tau ELISA Kit ab273617 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human Tau ELISA kit ab237617

Human Tau ELISA kit ab237617 has been used in the study of microglial surveillance in neurodegenerative diseases ⁽¹⁾.

Reference:

⁽¹⁾Dayuan Wang et al. 2022, PMID:36873190

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human/Mouse Tau Antibody Pair - BSA and Azide free ab253413
- Anti-Tau antibody [EPR22524-211] - BSA and Azide free (Capture) ab259552
- Anti-Tau antibody [EPR22521-17] - BSA and Azide free (Detector) ab259658

Tau proteins constitute nine isoforms from a single transcript from the MAPT gene that range from 33-81 kDa. Tau proteins are expressed mainly in the neurons of the central nervous systems. They promote microtubule assembly and stability and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus of Tau binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that Tau functions as a linker protein between both. Hyperphosphorylation of Tau proteins can result in destabilization of microtubule organization, Tau aggregation, and tangle formation. Defective Tau proteins may play a role in diseases of the nervous systems, including Alzheimer disease, Pick disease of the brain, Progressive supranuclear palsy 1 and Parkinson-dementia syndrome. Based on the immunogen design of this ELISA antibody pair, this kit should detect all nine human Tau isoforms and it should have equal affinity towards human, mouse and rat Tau proteins.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.