人Syndecan-1 ELISA试剂盒(CD138) (ab46506)
Key features and details
- Sensitivity: 4.94 ng/ml
- Range: 8 ng/ml - 256 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
-
产品名称
人Syndecan-1 ELISA试剂盒(CD138)
参阅全部 Syndecan-1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 6 6.2% 批次间 样品 n Mean SD CV% Overall 6 10.2% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 4.94 ng/ml -
范围
8 ng/ml - 256 ng/ml -
回收率
101 %
-
检测时间
1h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Human Syndecan-1 ELISA (CD138) kit is designed for the quantitative measurement of Syndecan-1 in Human serum, plasma, buffered solutions and supernatants.
A monoclonal antibody specific for Syndecan-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Syndecan-1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Syndecan-1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Syndecan-1 present in the samples.
This kit will recognize both endogenous and recombinant Human Syndecan-1.
-
经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-Syndecan-1 Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml Control Yellow 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml Plastic Plate Covers 2 units 4 units Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl Syndecan-1 Microplate (12 x 8 well strips) 1 unit 2 units Syndecan-1 Standard (lyophilized) Orange 2 vials 4 vials -
研究领域
-
功能
Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. -
序列相似性
Belongs to the syndecan proteoglycan family. -
细胞定位
Membrane. - Information by UniProt
-
别名
- CD_antigen
- CD138
- CD138 antigen
see all -
数据库链接
- Entrez Gene: 6382 Human
- Omim: 186355 Human
- SwissProt: P18827 Human
- Unigene: 224607 Human
- Unigene: 665958 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab46506于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
-
ELISA: Syndecan (CD138) Human ELISA Kit (ab46506)
Syndecan measured in undiluted cell culture supernatants, including the positive control from kit (duplicates +/- SD).
-
ELISA: Syndecan (CD138) Human ELISA Kit (ab46506)Syndecan measured in human biological fluids (dilution range 1/1-1/10); mouse and pig samples (plasma and serum) were below limit of detection (duplicates +/- SD).
-
ELISA: Syndecan (CD138) Human ELISA Kit (ab46506)Standard curve of human syndecan with background signal subtracted (duplicates; +/- SD).
-
Typical Standard CurveRepresentative Standard Curve using ab46506
数据表及文件
-
SDS download
-
Datasheet download
文献 (33)
ab46506 被引用在 33 文献中.
- Fernández-Sarmiento J et al. The association between prolonged capillary refill time and microcirculation changes in children with sepsis. BMC Pediatr 24:68 (2024). PubMed: 38245695
- Zhang Q et al. Effects of convalescent plasma infusion on the ADAMTS13-von Willebrand factor axis and endothelial integrity in patients with severe and critical COVID-19. Res Pract Thromb Haemost 7:100010 (2023). PubMed: 36531671
- Guo Y et al. Elevated serum immunoglobulin level predicts high risk of 1-year recurrence in patients with Takayasu arteritis. Arthritis Res Ther 25:36 (2023). PubMed: 36882846
- Druzak S et al. Multiplatform analyses reveal distinct drivers of systemic pathogenesis in adult versus pediatric severe acute COVID-19. Nat Commun 14:1638 (2023). PubMed: 37015925
- Miftode RS et al. Syndecan-1: From a Promising Novel Cardiac Biomarker to a Surrogate Early Predictor of Kidney and Liver Injury in Patients with Acute Heart Failure. Life (Basel) 13:N/A (2023). PubMed: 37109427