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AB316899

人Superoxide Dismutase 3/EC-SOD ELISA试剂盒

Human Superoxide Dismutase 3/EC-SOD ELISA Kit

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Human Superoxide Dismutase 3/EC-SOD ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Superoxide Dismutase 3/EC-SOD with a sensitivity of 21 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Extracellular superoxide dismutase [Cu-Zn], EC-SOD, SOD3

4 Images
Sandwich ELISA - Human Superoxide Dismutase 3/EC-SOD ELISA Kit (AB316899)
  • sELISA

Supplier Data

Sandwich ELISA - Human Superoxide Dismutase 3/EC-SOD ELISA Kit (AB316899)

Interpolated concentrations of Superoxide Dismutase 3/EC-SOD in normal human serum donors

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Superoxide Dismutase 3/EC-SOD concentration was determined to be 221 ng/mL with a range of 107 – 607 ng/mL.

Sandwich ELISA - Human Superoxide Dismutase 3/EC-SOD ELISA Kit (AB316899)
  • sELISA

Supplier Data

Sandwich ELISA - Human Superoxide Dismutase 3/EC-SOD ELISA Kit (AB316899)

Interpolated concentrations of human Superoxide Dismutase 3/EC-SOD in serum, plasma (citrate), plasma (EDTA), and plasma (heparin)

Interpolated concentration of native Superoxide Dismutase 3/EC-SOD was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1% serum, 2% plasma (citrate), 2% plasma (EDTA), and 2% plasma (heparin).  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Superoxide Dismutase 3/EC-SOD ELISA Kit (AB316899)
  • sELISA

Supplier Data

Sandwich ELISA - Human Superoxide Dismutase 3/EC-SOD ELISA Kit (AB316899)

Interpolated concentrations of human Superoxide Dismutase 3/EC-SOD in heart tissue extract

Interpolated concentration of native Superoxide Dismutase 3/EC-SOD was measured in duplicate at different sample concentrations. Undiluted samples are 20 µg/mL heart tissue extract.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Superoxide Dismutase 3/EC-SOD ELISA Kit (AB316899)
  • sELISA

Supplier Data

Sandwich ELISA - Human Superoxide Dismutase 3/EC-SOD ELISA Kit (AB316899)

Example of human Superoxide Dismutase 3/EC-SOD standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Tissue Extracts

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 21 pg/mL

范围

46.875 - 3000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human Superoxide Dismutase 3/EC-SOD SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Superoxide Dismutase 3/EC-SOD protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Tissue extracts. Quantitate Human Superoxide Dismutase 3/EC-SOD with 21 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" } ]
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回收率

[ { "sample": "Serum", "range": "91 - 108 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "103 - 107 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "93 - 101 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "103 - 116 %", "average": "= 108" }, { "sample": "Tissue Extracts", "range": "82 - 105 %", "average": "= 94" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 3 also known as EC-SOD is an antioxidant enzyme with a molecular weight of approximately 135 kDa. It's an extracellular enzyme that catalyzes the dismutation of superoxide radicals into hydrogen peroxide and molecular oxygen. EC-SOD is found mostly in the extracellular matrix tissues such as lungs blood vessels and heart. Its activity depends on the presence of metal ions copper and zinc leading its classification as a Cu/Zn SOD enzyme. Scientists often study EC-SOD using ELISA assays because of its importance in neutralizing reactive oxygen species.
Biological function summary

The enzyme acts as an important regulator of oxidative stress by reducing levels of superoxide radicals. EC-SOD forms a complex with heparan sulfate proteoglycans in the extracellular matrix which influences its stability and distribution. This enzyme plays a role in maintaining the redox balance influencing cell signaling inflammation and tissue damage which makes it important in managing oxidative stress in different tissues including the cardiovascular and respiratory systems.

Pathways

Superoxide dismutase 3 participates in antioxidant pathways important for cellular defense against oxidative stress. It is particularly important in the oxidative stress response pathway. By reducing superoxide radicals EC-SOD influences pathways involving other antioxidant proteins like catalase and glutathione peroxidase. These interactions help prevent the accumulation of harmful oxidants and maintain cellular homeostasis. EC-SOD's action connects closely with other superoxide dismutases such as Cu/Zn SOD and MnSOD within these protective pathways.

Enhanced or impaired EC-SOD function associates with various health issues such as cardiovascular diseases and chronic obstructive pulmonary disease (COPD). EC-SOD has been shown to protect vascular health by reducing oxidative stress related damages which can mitigate the risk of atherosclerosis. In the context of respiratory conditions like COPD alterations in EC-SOD expression can disrupt lung tissue function and inflammatory responses. Its interactions with proteins like catalase further contribute to its protective role against cell and tissue damage during disease progression.
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产品实验方案

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靶点信息

Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
See full target information Extracellular superoxide dismutase [Cu-Zn]
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文献 (1)

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Applications

Unspecified application

Species

Unspecified reactive species

Qian Kong,Qixiang Liang,Yinli Tan,Xiangqin Luo,Yesheng Ling,Xiaofeng Li,Yun Cai,Huiqin Chen
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