人Superoxide Dismutase 1 ELISA试剂盒(ab119520)
Key features and details
- Sensitivity: 0.04 ng/ml
- Range: 0.08 ng/ml - 5 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Superoxide Dismutase 1 ELISA试剂盒
参阅全部 Superoxide Dismutase 1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5.1% 批次间 样品 n Mean SD CV% Overall 5.8% -
样品类型
Cell culture supernatant, Urine, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
0.04 ng/ml -
范围
0.08 ng/ml - 5 ng/ml -
回收率
98 %
特定样本回收率 样品类型 平均% 范围 Serum 98 89% - 108% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human Superoxide Dismutase 1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cu/Zn Superoxide Dismutase concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin), amniotic fluid, fetal umbilical vein blood and urine.
Cu/Zn Superoxide Dismutase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a Cu/Zn Superoxide Dismutase HRP-conjugated detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the Cu/Zn Superoxide Dismutase amount of sample captured in plate.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 2 units HRP-Conjugate anti-Human Cu/Zn Superoxide Dismutase monoclonal antibody 2 x 20µl Human Cu/Zn Superoxide Dismutase Standard (5 ng/mL) 2 x 500µl Microplate coated with anti Human Cu/Zn SOD monoclonal antibody (12 x 8 wells) 1 unit Phosphate Buffered Saline Concentrate (PBS) (20x) 1 x 5ml Stop Solution (1M Phosphoric acid) 1 x 15ml TMB Substrate Solution 1 x 15ml -
研究领域
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细胞定位
Cytoplasmic. Note: The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria. -
别名
- ALS
- ALS1
- Amyotrophic Lateral Sclerosis 1 (Adult)
see all -
数据库链接
- Entrez Gene: 6647 Human
- Omim: 147450 Human
- SwissProt: P00441 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119520于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab119520.
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab119520 被引用在 13 文献中.
- Zhang Y et al. Notoginsenoside Fc alleviates oxidized low-density lipoprotein-induced endothelial cell dysfunction and upregulates PPAR-γ in vitro. Histol Histopathol 39:959-967 (2024). PubMed: 38193235
- Figueroa A et al. Combined Citrulline and Glutathione Supplementation Improves Endothelial Function and Blood Pressure Reactivity in Postmenopausal Women. Nutrients 15:N/A (2023). PubMed: 37049398
- Ju Y et al. Combining curcumin and aspirin ameliorates preeclampsia-like symptoms by inhibiting the placental TLR4/NF-κB signaling pathway in rats. J Obstet Gynaecol Res 49:128-140 (2023). PubMed: 36288911
- Lee H et al. Lactobacillus paracasei HY7015 and Lycopus lucidus Turcz. Extract Promotes Human Dermal Papilla Cell Cytoprotective Effect and Hair Regrowth Rate in C57BL/6 Mice. Molecules 27:N/A (2022). PubMed: 36500327
- Li R et al. Glucose Starvation-Caused Oxidative Stress Induces Inflammation and Autophagy in Human Gingival Fibroblasts. Antioxidants (Basel) 11:N/A (2022). PubMed: 36290630