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AB126429

人STAT5 (Tyr694) In-Cell ELISA试剂盒

Human STAT5 (Tyr694) In-Cell ELISA Kit

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Human STAT5 (Tyr694) In-Cell ELISA Kit is a ELISA for the measurement of Human STAT5 (Tyr694) In-Cell in Human in Cell Samples samples.

查看别名

STAT5, STAT5A, Signal transducer and activator of transcription 5A

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In-Cell ELISA - Human STAT5 (Tyr694) In-Cell ELISA Kit (AB126429)
  • In-Cell ELISA

Unknown

In-Cell ELISA - Human STAT5 (Tyr694) In-Cell ELISA Kit (AB126429)

A431 cells were stimulated by different concentrations of EGF for 10 minutes at 37°C.

In-Cell ELISA - Human STAT5 (Tyr694) In-Cell ELISA Kit (AB126429)
  • In-Cell ELISA

Unknown

In-Cell ELISA - Human STAT5 (Tyr694) In-Cell ELISA Kit (AB126429)

A431 cells were stimulated by different concentrations of EGF for 30 minutes at 37°C.

Western blot - Human STAT5 (Tyr694) In-Cell ELISA Kit (AB126429)
  • WB

Supplier Data

Western blot - Human STAT5 (Tyr694) In-Cell ELISA Kit (AB126429)

Western blot analysis of extracts from 100 ng/ml hEGF treated A431 cells. Phospho-STAT5 (Tyr694) and STAT5 antibodies were used in both detection assays.

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关键信息

检测方法

Colorimetric

样品类型

Adherent cells

反应种属

Human

Semi-Quantitative
检测时间

5h 10m

检测平台

Microplate

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搭配使用产品

Assay Kits

AB205714

STAT 5 A/B ELISA Kit

SimpleStep

Sandwich ELISA - STAT 5 A/B ELISA Kit (AB205714)

  • 5
  • 1
Assay Kits

AB176656

STAT5 A/B pY694/699 ELISA Kit

SimpleStep

Sandwich ELISA - STAT5 A/B pY694/699 ELISA Kit (AB176656)

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Assay Kits

AB279945

Phosphotyrosine STAT5 ELISA Kit

Sandwich ELISA - Phosphotyrosine STAT5 ELISA Kit (AB279945)

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Primary Antibodies

AB278764

Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11]

20ul selling size|Recombinant

Flow Cytometry - Anti-STAT5 (phospho Y694) antibody [Stat5Y694-G11] (AB278764)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

ab126429 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of STAT5 (Tyr694) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human cell lines. By determining STAT5 protein phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort in preparing cell lysate and performing an analysis of Western Blot.

In the In-Cell STAT5 (Tyr694) ELISA kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, Anti Phospho-STAT5 (Tyr694) or Anti-STAT5 (primary antibody) is pipetted into the wells and incubated. The wells are washed, and HRP-conjugated anti-mouse IgG (secondary antibody) is added to the wells. The wells are washed again, a TMB substrate solution is added to the wells and color develops in proportion to the amount of protein. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab126429-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Wash Buffer B Concentrate (20X)", "number":"AB126429-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"Fixing Solution", "number":"AB126429-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB126429-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Blocking Buffer Concentrate (5X)", "number":"AB126429-CMP04", "productcode":"" }, { "size":"1 x 2 mL", "name":"Quenching Buffer Concentrate (30x)", "number":"AB126429-CMP03", "productcode":"" }, { "size":"1 x 14 mL", "name":"Stop Solution", "number":"AB126429-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Uncoated 96-well Microplate", "number":"AB126429-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Wash Buffer A Concentrate (20X)", "number":"AB126429-CMP02", "productcode":"" }, { "size":"1 x 10 µL", "name":"HRP-conjugated Anti-Mouse IgG Concentrate", "number":"AB126429-CMP11", "productcode":"" }, { "size":"1 x 10 µL", "name":"Mouse Anti-STAT5 Concentrate (Item H)", "number":"AB126429-CMP08", "productcode":"" }, { "size":"1 x 7 µL", "name":"Mouse Anti-Phospho-STAT5 (Tyr694) Concentrate (Item G)", "number":"AB126429-CMP10", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 5 commonly known as STAT5 is a member of the STAT protein family. It weighs approximately 94 kDa. STAT5 usually exists in two closely related forms STAT5a and STAT5b with both playing similar roles but having slightly different activity. This protein is widely expressed found in cells like lymphocytes epithelial cells and endothelial cells indicating its importance in various tissues.
Biological function summary

STAT5 acts as a transcription factor that modulates gene expression by binding to specific DNA sequences. It often forms part of a larger complex when activated. This complex relocation to the nucleus triggers transcriptional activities essential for cell growth differentiation and survival. As it interacts with other proteins STAT5 plays an important role in cytokine signaling particularly in response to interleukins and growth hormones.

Pathways

STAT5 is important in the JAK-STAT signaling pathway. This pathway is activated by cytokines and growth factors leading to STAT5 phosphorylation and its subsequent translocation into the nucleus. The interaction with Janus kinases (JAKs) facilitates this process. STAT5 also plays a significant role in the MAPK pathway linking it to various cellular responses and functions.

STAT5 has a strong association with oncological conditions like leukemia and lymphomas. Aberrant activation or dysregulation of STAT5 can lead to uncontrolled cell proliferation and survival contributing to cancer. Furthermore STAT5 is linked with autoimmune diseases with its malfunction affecting immune response and leading to disorders. In these conditions proteins like BCL-XL often interact with STAT5 influencing cell survival pathways.
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产品实验方案

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靶点信息

Carries out a dual function : signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
See full target information STAT5A pY694
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websiteProtocolBooklet
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