人Stabilin-1 ELISA试剂盒(ab317782)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 33.574 pg/ml
- Range: 171.875 pg/ml - 11000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Stabilin-1 ELISA试剂盒
参阅全部 stabilin1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 8 8.3% 批次间 样品 n Mean SD CV% Serum 3 8.4% -
样品类型
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
33.574 pg/ml -
范围
171.875 pg/ml - 11000 pg/ml -
回收率
114 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 111 104% - 115% Serum 114 109% - 120% Hep Plasma 106 100% - 117% EDTA Plasma 112 89% - 125% Cit plasma 117 114% - 122% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human Stabilin-1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Stabilin-1 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Stabilin-1 with 33.574 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 1 x 384 tests 10 x 96 tests 10X Human Stabilin-1 Capture Antibody 1 x 600µl 1 x 600µl 10 x 600µl 10X Human Stabilin-1 Detector Antibody 1 x 600µl 1 x 600µl 10 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml 1 x 200ml 384 well CaptSure™ microplates 0 x 0 unit 1 unit 0 x 0 unit Antibody Diluent 4BI 1 x 6ml 1 x 6ml 10 x 6ml Human Stabilin-1 Lyophilized Recombinant Protein 2 vials 2 vials 20 vials Plate Seal 1 unit 1 unit 10 units Sample Diluent NS (ab193972) 1 x 50ml 1 x 50ml 2 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 0 x 0 unit 10 units Stop Solution 1 x 12ml 2 x 12ml 1 x 120ml TMB Development Solution 1 x 12ml 2 x 12ml 1 x 120ml -
研究领域
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功能
Acts as a scavenger receptor for acetylated low density lipoprotein. Binds to both Gram-positive and Gram-negative bacteria and may play a role in defense against bacterial infection. When inhibited in endothelial tube formation assays, there is a marked decrease in cell-cell interactions, suggesting a role in angiogenesis. Involved in the delivery of newly synthesized CHID1/SI-CLP from the biosynthetic compartment to the endosomal/lysosomal system. -
组织特异性
High levels found in spleen, lymph node, liver and placenta. Also expressed in endothelial cells. -
序列相似性
Contains 16 EGF-like domains.
Contains 7 FAS1 domains.
Contains 2 laminin EGF-like domains.
Contains 1 Link domain. -
细胞定位
Membrane. - Information by UniProt
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别名
- CLEVER 1
- Common lymphatic endothelial and vascular endothelial receptor 1
- EGF-like
see all -
数据库链接
- Entrez Gene: 23166 Human
- Omim: 608560 Human
- SwissProt: Q9NY15 Human
- Unigene: 301989 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317782于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example of human Stabilin-1 standard curve in Sample Diluent NS.Example of human Stabilin-1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of human Stabilin-1 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).Interpolated concentration of native Stabilin-1 was measured in duplicate at different sample concentrations. Undiluted samples are 2.5% for serum, 2.5% plasma (citrate), 5% plasma (EDTA) and 5% plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Interpolated concentrations of human Stabilin-1 in HUVEC cell culture supernatant.Interpolated concentration of native Stabilin-1 was measured in duplicate at different sample concentrations. Undiluted samples are 100% HUVEC cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. -
Interpolated concentrations of human Stabilin-1 in normal human serum donors.Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Stabilin-1 concentration was determined to be 226,774 pg/mL with a range of 183,511 - 327,540 pg/mL.
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Example of human Stabilin-1 standard curve in Sample Diluent NS in 96-well vs. 384-well plate.Example of human Stabilin-1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of human Stabilin-1 in serum in 96-well vs. 384-well plates.Interpolated concentration of native Stabilin-1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5% serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. -
Human Stabilin-1 ELISA Kit (ab317782)To learn more about the advantages of SimpleStep ELISA® kits see here.
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Human Stabilin-1 ELISA Kit (ab317782)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Human Stabilin-1 ELISA Kit (ab317782)To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
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