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AB317782

人Stabilin-1 ELISA试剂盒

Human Stabilin-1 ELISA Kit

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Human Stabilin-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Stabilin-1 with a sensitivity of 33.574 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

FEEL1, KIAA0246, STAB1, Stabilin-1, MS-1 antigen, FEEL-1

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Sandwich ELISA - Human Stabilin-1 ELISA Kit (AB317782)
  • sELISA

Supplier Data

Sandwich ELISA - Human Stabilin-1 ELISA Kit (AB317782)

Interpolated concentrations of human Stabilin-1 in serum in 96-well vs. 384-well plates. Interpolated concentration of native Stabilin-1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5% serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Stabilin-1 ELISA Kit (AB317782)
  • sELISA

Supplier Data

Sandwich ELISA - Human Stabilin-1 ELISA Kit (AB317782)

Interpolated concentrations of human Stabilin-1 in HUVEC cell culture supernatant. Interpolated concentration of native Stabilin-1 was measured in duplicate at different sample concentrations. Undiluted samples are 100% HUVEC cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Stabilin-1 ELISA Kit (AB317782)
  • sELISA

Supplier Data

Sandwich ELISA - Human Stabilin-1 ELISA Kit (AB317782)

Interpolated concentrations of human Stabilin-1 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native Stabilin-1 was measured in duplicate at different sample concentrations. Undiluted samples are 2.5% for serum, 2.5% plasma (citrate), 5% plasma (EDTA) and 5% plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Stabilin-1 ELISA Kit (AB317782)
  • sELISA

Supplier Data

Sandwich ELISA - Human Stabilin-1 ELISA Kit (AB317782)

Interpolated concentrations of human Stabilin-1 in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Stabilin-1 concentration was determined to be 226,774 pg/mL with a range of 183,511 - 327,540 pg/mL.

Sandwich ELISA - Human Stabilin-1 ELISA Kit (AB317782)
  • sELISA

Supplier Data

Sandwich ELISA - Human Stabilin-1 ELISA Kit (AB317782)

Example of human Stabilin-1 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Stabilin-1 ELISA Kit (AB317782)
  • sELISA

Supplier Data

Sandwich ELISA - Human Stabilin-1 ELISA Kit (AB317782)

Example of human Stabilin-1 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Serum, EDTA Plasma, Cell culture supernatant, Citrate plasma, Heparin Plasma

反应种属

Human

检测类型

Sandwich

灵敏度

= 33.574 pg/mL

范围

171.875 - 11000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human Stabilin-1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Stabilin-1 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Stabilin-1 with 33.574 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.4" } ]
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回收率

[ { "sample": "Serum", "range": "109 - 120 %", "average": "= 114" }, { "sample": "Citrate plasma", "range": "114 - 122 %", "average": "= 117" }, { "sample": "EDTA Plasma", "range": "89 - 125 %", "average": "= 112" }, { "sample": "Heparin Plasma", "range": "100 - 117 %", "average": "= 106" }, { "sample": "Cell culture supernatant", "range": "104 - 115 %", "average": "= 111" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Acts as a scavenger receptor for acetylated low density lipoprotein. Binds to both Gram-positive and Gram-negative bacteria and may play a role in defense against bacterial infection. When inhibited in endothelial tube formation assays, there is a marked decrease in cell-cell interactions, suggesting a role in angiogenesis. Involved in the delivery of newly synthesized CHID1/SI-CLP from the biosynthetic compartment to the endosomal/lysosomal system.
See full target information STAB1
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websiteProtocolBooklet
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搭配使用产品

Primary Antibodies

AB317257

Anti-Stabilin-1 antibody [EPR27955-12] - BSA and Azide free

BOND RX™ Validated|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stabilin-1 antibody [EPR27955-12] - BSA and Azide free (AB317257)

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