人SPARCL1 ELISA试剂盒(SPARC-like蛋白1) (ab272478)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.89 pg/ml
- Range: 39.06 pg/ml - 2500 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人SPARCL1 ELISA试剂盒(SPARC-like蛋白1)
参阅全部 SPARCL1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 8 3.3% 批次间 样品 n Mean SD CV% Serum 3 3.27% -
样品类型
Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
7.89 pg/ml -
范围
39.06 pg/ml - 2500 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 92 89% - 95% Tissue Extracts 105 104% - 106% Hep Plasma 99 93% - 108% EDTA Plasma 99 96% - 104% Cit plasma 102 92% - 116% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human SPARCL1 ELISA kit (ab272478) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human SPARCL1 protein in serum, plasma and cell and tissue culture extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SPARCL1 with 7.89 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a 75 kD protein that is encoded by the SPARCL1 gene. SPARCL1 is highly expressed in high endothelial venules (HEVs) and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration. SPARCL1 is also highly expressed in heart, lung, brain, skeletal muscle, ovary, colon, and small intestine tissues. SPARCL1 may have implications in the regulation of cell adhesion, migration and proliferation. The antibody pair was designed against the mature full-length extracellular fragment of SPARCL1. SPARCL1 shares 64%, 64%, 72%, and 93% sequence homology in mouse, rat, cow, and monkey, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human SPARCL1 (SPARC-like protein 1) Capture Antibody 1 x 600µl 10X Human SPARCL1 (SPARC-like protein 1) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI2 1 x 6ml Human SPARCL1 (SPARC-like protein 1) Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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组织特异性
Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes. -
序列相似性
Belongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain. -
翻译后修饰
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. Thr-398 is the preferred site to Thr-399. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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别名
- Ecm2
- Extracellular matrix protein 2
- Hevin
see all -
数据库链接
- Entrez Gene: 8404 Human
- Omim: 606041 Human
- SwissProt: Q14515 Human
- Unigene: 62886 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272478于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human SPARCL1 standard curve in Sample Diluent NS. -
Interpolated concentrations of native SPARCL1 in human serum and plasma samples.The concentrations of SPARCL1 were measured in duplicates, interpolated from the SPARCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:100, plasma (citrate) 1:200, plasma (EDTA) 1:100, and plasma (heparin) 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SPARCL1 concentration was determined to be 215.28 ng/mL in serum, 468.22 ng/mL in plasma (citrate), 231.62 ng/mL in plasma (EDTA), and 428.58 ng/mL in plasma (heparin).
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Interpolated concentrations of native SPARCL1 in human brain tissuebased on a 25 μg/mL extract load and human lung based on a 250 μg/mL extract load. The concentrations of SPARCL1 were measured in duplicate and interpolated from the SPARCL1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SPARCL1 concentration was determined to be 519.79 pg/mL in brain extract and 108.09 pg/mL in lung extract.
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Sandwich ELISA - Human SPARCL1 ELISA Kit (SPARC-like protein 1) (ab272478)To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
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