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AB245707

人SOX2 ELISA试剂盒

Human SOX2 ELISA Kit

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(2 Publications)

Human SOX2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SOX2 with a sensitivity of 31 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Transcription factor SOX-2, SOX2

3 Images
ELISA - Human SOX2 ELISA Kit (AB245707)
  • ELISA

Supplier Data

ELISA - Human SOX2 ELISA Kit (AB245707)
Sandwich ELISA - Human SOX2 ELISA Kit (AB245707)
  • sELISA

Supplier Data

Sandwich ELISA - Human SOX2 ELISA Kit (AB245707)

Interpolated concentrations of native SOX2 in human NCCIT cell extracts based on a 50 μg/mL extract load. The concentrations of SOX2 were measured in duplicate and interpolated from the SOX2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SOX2 concentration was determined to be 36.48 ng/mL.

Sandwich ELISA - Human SOX2 ELISA Kit (AB245707)
  • sELISA

Supplier Data

Sandwich ELISA - Human SOX2 ELISA Kit (AB245707)

Example of human SOX2 standard curve in 1X Cell Extraction Buffer PTR. The SOX2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell culture extracts

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 31 pg/mL

范围

0.46875 - 30 ng/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

Assay Kits

AB235653

Human/Mouse Oct4 ELISA Kit

Recombinant|SimpleStep

ELISA - Human/Mouse Oct4 ELISA Kit (AB235653)

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Assay Kits

AB246527

Human KLF4 ELISA Kit

Recombinant|SimpleStep

ELISA - Human KLF4 ELISA Kit (AB246527)

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Assay Kits

AB277452

Human c-Myc ELISA Kit

ELISA - Human c-Myc ELISA Kit (AB277452)

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Primary Antibodies

AB92494

Anti-SOX2 antibody [EPR3131]

RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (AB92494)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human SOX2 ELISA Kit ab245707 is a rapid single-wash 90-min Sandwich ELISA to measure Human SOX2 in cell culture extracts. This SimpleStep sensitivity is 31 pg/mL.

How the assay works

Human SOX2 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human SOX2 ELISA Kit ab245707 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-SOX2 antibody [EPR20531-102] - BSA and Azide free (Capture) ab252657
- Anti-SOX2 antibody [EPR20531-143] - BSA and Azide free (Detector) ab252657

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]
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回收率

[ { "sample": "Cell culture extracts", "range": "79 - 98 %", "average": "= 88" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab245707-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB245707-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB245707-CMP13", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB245707-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB245707-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB245707-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB245707-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB245707-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB245707-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human SOX2 Capture Antibody", "number":"AB245707-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human SOX2 Lyophilized Recombinant Protein", "number":"AB245707-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human SOX2 Detector Antibody", "number":"AB245707-CMP03", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed : 18035408). Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity).
See full target information SOX2
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40422249

2025

All-Trans Retinoic Acid Induces Differentiation and Downregulates Stemness Markers and MGMT Expression in Glioblastoma Stem Cells.

Applications

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Species

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Justin Tang,Raymond Yang

Nature communications 14:3366 PubMed37291242

2023

Bioinspired engineering of fusogen and targeting moiety equipped nanovesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Lixue Wang,Guosheng Wang,Wenjun Mao,Yundi Chen,Md Mofizur Rahman,Chuandong Zhu,Peter M Prisinzano,Bo Kong,Jing Wang,Luke P Lee,Yuan Wan
View all publications
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