人Serpin B2 ELISA试剂盒(PAI-2) (ab272480)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 110 pg/ml
- Range: 312.5 pg/ml - 20000 pg/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Serpin B2 ELISA试剂盒(PAI-2)
参阅全部 SerpinB2/PAI-2 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Protein 8 2.8% 批次间 样品 n Mean SD CV% Protein 3 4.24% -
样品类型
Tissue Extracts, Cell Lysate -
检测类型
Sandwich (quantitative) -
灵敏度
110 pg/ml -
范围
312.5 pg/ml - 20000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Tissue Extracts 84 80% - 90% Cell Lysate 85 85% - 85% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human Serpin B2 ELISA kit (ab272480) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Serpin B2 protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Serpin B2 with 110 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Serpin B2, also known as plasminogen activator inhibitor-2 (PAI-2), is a protein that belongs to the serpin family of protease inhibitors. Serpin B2 exists in two forms, a 47kDa immature form and glycolsylated 60 kDa mature. The majority of clade B (ov-serpins) of the serpin family inhibit serine and/or papain-like cysteine proteinases and protect cells from exogenous and endogenous proteinase-mediated injury. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1. Serpin B2 shares 76% and 73% sequence homology with mouse and rat, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human Serpin B2 (PAI-2) Capture Antibody 1 x 600µl 10X Human Serpin B2 (PAI-2) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human Serpin B2 (PAI-2) Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1. -
序列相似性
Belongs to the serpin family. Ov-serpin subfamily. -
翻译后修饰
The signal sequence is not cleaved. -
细胞定位
Cytoplasm. Secreted > extracellular space. - Information by UniProt
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别名
- HsT1201
- Monocyte Arg serpin
- Monocyte Arg-serpin
see all -
数据库链接
- Entrez Gene: 5055 Human
- Omim: 173390 Human
- SwissProt: P05120 Human
- Unigene: 594481 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272480于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example of human Serpin B2 standard curve in 1X Cell Extraction Buffer PTR.
The Serpin B2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Serpin B2 in human A431 cellsbased on a 200 μg/mL extract load and placenta tissue extract based on a 30 μg/mL extract load. The concentrations of Serpin B2 were measured in duplicate and interpolated from the Serpin B2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serpin B2 concentration was determined to be 18.93 ng/mL in A431 extract and 18.79 ng/mL in placenta extract.
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Sandwich ELISA - Human Serpin B2 ELISA Kit (PAI-2) (ab272480)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - Human Serpin B2 ELISA Kit (PAI-2) (ab272480)To learn more about the advantages of recombinant antibodies see here. -
Sandwich ELISA - Human Serpin B2 ELISA Kit (PAI-2) (ab272480)To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
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