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AB100637

人SDF1 alpha ELISA试剂盒

Human SDF1 ELISA Kit

5

(2 Reviews)

|

(18 Publications)

Human SDF1 ELISA Kit is a sandwich ELISA designed to quantify Human SDF1 with a sensitivity of 42 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 42 - 6000 pg/mL
- Cited in over 10 publications

查看别名

SDF1, SDF1A, SDF1B, CXCL12, Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor, IRH, hIRH, PBSF

2 Images
Sandwich ELISA - Human SDF1 ELISA Kit (AB100637)
  • sELISA

Unknown

Sandwich ELISA - Human SDF1 ELISA Kit (AB100637)

Representative standard curve using ab100637

Sandwich ELISA - Human SDF1 ELISA Kit (AB100637)
  • sELISA

Unknown

Sandwich ELISA - Human SDF1 ELISA Kit (AB100637)

Representative standard curve using ab100637

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 42 pg/mL

范围

42 - 6000 pg/mL

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human SDF1 ELISA Kit ab100637 is a sandwich ELISA to measure Human SDF1 in plasma, cell culture supernatant with a sensitivity of 42 pg/ml.

How the assay works

This assay employs an antibody specific for Human SDF1 coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SDF1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human SDF1 ELISA Kit ab100637 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Optimization may be required with urine samples.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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回收率

[ { "sample": "Cell culture supernatant", "range": "86 - 104 %", "average": "= 94.63" }, { "sample": "Plasma", "range": "85 - 104 %", "average": "= 89.34" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100637-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100637-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Microplate (12 x 8 wells)", "number":"AB100637-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100637-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100637-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100637-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100637-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human SDF1", "number":"AB100637-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human SDF1 Standard (lyophilized)", "number":"AB100637-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100637-CMP05", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SDF1 beta and SDF1 alpha also known as CXCL12 are small chemokines playing important roles in several cellular processes. These signaling proteins have a molecular mass ranging from approximately 7 to 15 kDa depending on post-translational modifications. They are secreted proteins expressed in various tissues including bone marrow heart liver and brain. SDF1 beta and SDF1 alpha function by binding to the chemokine receptor CXCR4 mediating cellular responses through signal transduction mechanisms.
Biological function summary

SDF1 beta and SDF1 alpha regulate hematopoietic cell trafficking and homing. These proteins are significant for directing migration of immune cells and stem cells by forming part of a ligand-receptor complex with CXCR4. This interaction influences cell survival proliferation and inflammation. SDF1 also participates in organ development and promotes angiogenesis during tissue repair.

Pathways

SDF1 beta and SDF1 alpha are central to the CXCL12-CXCR4 axis important in signaling pathways like G-protein coupled receptor signaling and JAK-STAT signaling. These pathways contribute to immune response modulation and stem cell maintenance. Notably the interaction with CXCR4 is essential in the SDF-CXCR4 axis and effects are also mediated with other proteins like CXCR7 further impacting cellular signaling.

SDF1 beta and SDF1 alpha have associations with cancer metastasis and HIV infection. The SDF1-CXCR4 interaction represents an important point in metastatic spread since it aids tumor cell migration and invasion. This chemokine binding also serves as an essential co-receptor for HIV entry into cells implicating it in disease progression. Alterations in the SDF1-CXCR4 axis connect to oncogenic processes and viral infections alongside proteins such as CCR5 contributing to HIV pathway interactions.
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产品实验方案

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靶点信息

Chemoattractant active on T-lymphocytes and monocytes but not neutrophils (PubMed : 18802065, PubMed : 39093700). Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis (PubMed : 8752281, PubMed : 18802065, PubMed : 39093700). Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for CXCL12/SDF-1 (PubMed : 16107333, PubMed : 19255243). Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV : ITGB3, ITGA4 : ITGB1 and ITGA5 : ITGB1 in a CXCR4-independent manner (PubMed : 29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase (PubMed : 18802065). Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins (PubMed : 16107333, PubMed : 18802065, PubMed : 19255243, PubMed : 39093700). CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase (PubMed : 18802065). Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1 (PubMed : 8752281). Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation (By similarity). Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (By similarity).. SDF-1-beta(3-72). Shows a reduced chemotactic activity.. SDF-1-alpha(3-67). Shows a reduced chemotactic activity (PubMed : 14525775). Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites (PubMed : 14525775).
See full target information CXCL12
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文献 (18)

Recent publications for all applications. Explore the full list and refine your search

Angiogenesis 28:26 PubMed40314870

2025

SDF-1α mRNA therapy in peripheral artery disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tinghong Zhang,Binqiang Zhu,Shijie Deng,Jinling Qin,Jingyuan Zhang,Shu Meng

Frontiers in surgery 12:1507982 PubMed40166622

2025

Comparison of clinical efficacy between tibial cortex transverse transport and platelet-rich plasma treatment for severe diabetic foot ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Pu-Xiang Zhen,Hong-Jie Su,Si-Jie Yang,Xiang Chen,Zhan-Ming Lin,Sai-Nan Liu

Biomedicines 11: PubMed37189610

2023

Dimethyloxalylglycine Attenuates Steroid-Associated Endothelial Progenitor Cell Impairment and Osteonecrosis of the Femoral Head by Regulating the HIF-1α Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenkai Shao,Zilin Li,Bo Wang,Song Gong,Ping Wang,Beite Song,Zhixiang Chen,Yong Feng

Frontiers in immunology 13:1037812 PubMed36451827

2022

Increased peripheral inflammatory responses in myelin oligodendrocyte glycoprotein associated disease and aquaporin-4 antibody positive neuromyelitis optica spectrum disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Angelika Bauer,Dagmar Rudzki,Klaus Berek,Alessandro Dinoto,Christian Lechner,Eva-Maria Wendel,Harald Hegen,Florian Deisenhammer,Thomas Berger,Romana Höftberger,Kevin Rostasy,Sara Mariotto,Markus Reindl

EMBO reports 23:e55871 PubMed36200589

2022

Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yi Ng,Khang Leng Lee,Mark Dhinesh Muthiah,Kan Xing Wu,Florence Wen Jing Chioh,Konstanze Tan,Gwyneth Shook Ting Soon,Asim Shabbir,Wai Mun Loo,Zun Siong Low,Qingfeng Chen,Walter Wahli,Nguan Soon Tan,Huck Hui Ng,Yock Young Dan,Christine Cheung

Animal cells and systems 26:148-157 PubMed36046033

2022

CXCL12 secreted by pancreatic stellate cells accelerates gemcitabine resistance of pancreatic cancer by enhancing glycolytic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Lu,Yilei Wu,Rui Cao,Xiaojiong Yu,Jun Gong

EMBO reports 23:e54271 PubMed35403791

2022

Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yi Ng,Khang Leng Lee,Mark Dhinesh Muthiah,Kan Xing Wu,Florence Wen Jing Chioh,Konstanze Tan,Gwyneth Shook Ting Soon,Asim Shabbir,Wai Mun Loo,Zun Siong Low,Qingfeng Chen,Nguan Soon Tan,Huck Hui Ng,Yock Young Dan,Christine Cheung

Molecular medicine reports 22:4163-4172 PubMed32901877

2020

SDF1/CXCR4 axis facilitates the angiogenesis via activating the PI3K/AKT pathway in degenerated discs.

Applications

Unspecified application

Species

Unspecified reactive species

Hanxiang Zhang,Peng Wang,Xiang Zhang,Wenrui Zhao,Honglei Ren,Zhenming Hu

Medicine 99:e20944 PubMed32629699

2020

Inhibition of ITGB1 enhance the anti-tumor effect of cetuximab in colorectal cancer cell.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Yang,Shuai Wang,Weihua Yu,Yixiong Zheng,Yulian Wu

Stem cells international 2020:2803747 PubMed32377202

2020

Enhanced Effect of IL-1-Activated Adipose-Derived MSCs (ADMSCs) on Repair of Intestinal Ischemia-Reperfusion Injury via COX-2-PGE Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Liu,Yi Ren He,Shao Jun Liu,Lei Hu,Li Chuang Liang,Dong Liang Liu,Lin Liu,Zhi Qiang Zhu
View all publications
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