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AB137970

人SAP ELISA试剂盒(APCS)

Human SAP ELISA Kit (APCS)

5

(1 Review)

|

(2 Publications)

Human SAP ELISA Kit (APCS) is a Sandwich (quantitative) ELISA for the measurement of Human SAP (APCS) in Human in Biofluids samples.

查看别名

PTX2, APCS, Serum amyloid P-component, SAP, 9.5S alpha-1-glycoprotein

2 Images
Sandwich ELISA - Human SAP ELISA Kit (APCS) (AB137970)
  • sELISA

Lab

Sandwich ELISA - Human SAP ELISA Kit (APCS) (AB137970)

SAP measured in various samples showing quantity (microgram) per mL of tested sample

Sandwich ELISA - Human SAP ELISA Kit (APCS) (AB137970)
  • sELISA

Supplier Data

Sandwich ELISA - Human SAP ELISA Kit (APCS) (AB137970)

Representative Standard Curve using ab137970.

关键信息

检测方法

Colorimetric

样品类型

Urine, Plasma, Serum, Cerebral Spinal Fluid

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

> 24 pg/mL

范围

0.156 - 10 ng/mL

检测时间

4h

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human SAP (APCS) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human SAP in plasma, serum, urine, and CSF.

A SAP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a SAP specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of SAP captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.4" } ]
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回收率

[ { "sample": "Serum", "range": "97 - 104 %", "average": null }, { "sample": "Plasma", "range": "88 - 109 %", "average": null } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab137970-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB137970-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB137970-CMP08", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB137970-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SAP Microplate (12 x 8 well strips)", "number":"AB137970-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB137970-CMP01", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB137970-CMP09", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human SAP Antibody", "number":"AB137970-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"SAP Standard", "number":"AB137970-CMP06", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB137970-CMP03", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
Multi
储存信息
Please refer to protocols
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Serum Amyloid P component also known as SAP P-SAP or APCS protein is a pentraxin family member. It has a molecular mass of approximately 25 kDa. SAP expresses mainly in the liver and circulates in blood plasma. The protein consists of five identical subunits which form a homopentameric structure. This structure enables SAP to bind to amyloid fibrils a defining mechanical action of this protein.
Biological function summary

SAP plays a role in the immune system by recognizing damaged cells and pathogens. It acts as an opsonin marking these cellular targets for phagocytosis. As part of a complex SAP stabilizes extracellular matrix and amyloid deposits. It binds specifically to amyloid fibrils found in various tissues potentially facilitating their removal by immune cells. The protein's binding actions are important for its role in maintaining tissue and fluid homeostasis.

Pathways

SAP is actively involved in the innate immune response and amyloidogenic pathways. It interacts with the complement system to enhance the clearance of cell debris and amyloid fibrils. The complement system is an important component of innate immunity where SAP can engage with complement proteins like C1q. This interaction suggests that SAP plays roles in modulating both inflammation and tissue repair processes.

SAP has significant connections. SAP is related to systemic amyloidosis where it binds to amyloid deposits affecting multiple organs. Moreover SAP has been implicated in Alzheimer's disease due to its interaction with beta-amyloid protein plaques. In these disorders the involvement of SAP aids researchers in understanding the pathological mechanisms and could open avenues for targeted therapies aimed at modulating SAP functions.
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产品实验方案

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靶点信息

Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
See full target information APCS
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

RSC advances 8:10598-10609 PubMed35540476

2018

Identification of potential serum biomarkers of acute paraquat poisoning in humans using an iTRAQ quantitative proteomic.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Wei,Yi Wang,Ling Lin,Lei Zhang,Yan Shi,Ping Xiang,Shujun Cao,Min Shen,Pengyuan Yang

Proteomics. Clinical applications 11: PubMed28261998

2017

Use of a Targeted Urine Proteome Assay (TUPA) to identify protein biomarkers of delayed recovery after kidney transplant.

Applications

Unspecified application

Species

Unspecified reactive species

Kenneth R Williams,Christopher M Colangelo,Lin Hou,Lisa Chung,Justin M Belcher,Thomas Abbott,Isaac E Hall,Hongyu Zhao,Lloyd G Cantley,Chirag R Parikh
View all publications
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