AB282299

人S100A12/CGRP ELISA试剂盒

Human S100A12/CGRP ELISA Kit

Recombinant
SimpleStep
了解详情

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(2 Publications)

Human S100A12/CGRP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human S100A12/CGRP with a sensitivity of 1.89 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Protein S100-A12, CGRP, Calcium-binding protein in amniotic fluid 1, Calgranulin-C, Extracellular newly identified RAGE-binding protein, Migration inhibitory factor-related protein 6, Neutrophil S100 protein, S100 calcium-binding protein A12, CAAF1, CAGC, EN-RAGE, MRP-6, p6, S100A12

4 Images

ELISA - Human S100A12/CGRP ELISA Kit (AB282299)

ELISA

Supplier Data

ELISA - Human S100A12/CGRP ELISA Kit (AB282299)

sELISA

Supplier Data

Sandwich ELISA - Human S100A12/CGRP ELISA Kit (AB282299)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean S100A12/CGRP concentration was determined to be 2,155 pg/mL with a range of 1,217 – 39,456 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human S100A12/CGRP ELISA Kit (AB282299)

Interpolated concentrations of native S100A12/CGRP in human serum and plasma samples.

The concentrations of S100A12/CGRP were measured in duplicates, interpolated from the S100A12/CGRP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 20, plasma (citrate) 1 : 8, plasma (EDTA) 1 : 8, and plasma (heparin) 1 : 16. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean S100A12/CGRP concentration was determined to be 6,428 pg/mL in serum, 5,285 pg/mL in plasma (citrate), 3,163 pg/mL in plasma (EDTA), and 2,034 pg/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human S100A12/CGRP ELISA Kit (AB282299)

Example of human S100A12/CGRP standard curve in Sample Diluent NS.

The S100A12/CGRP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich

灵敏度

= 1.89 pg/mL

范围

7.81 - 500 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human S100A12/CGRP ELISA Kit ab282299 is a rapid single-wash 90-min Sandwich ELISA to measure Human S100A12/CGRP in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.89 pg/mL.

How the assay works

Human S100A12/CGRP SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human S100A12/CGRP ELISA Kit ab282299 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-S100A12/CGRP antibody [EPR23677-121] - BSA and Azide free (Capture) ab281062
- Anti-S100A12/CGRP antibody [EPR23677-102] - BSA and Azide free (Detector) ab281062

S100A12, also known as CCRP and calgranulin C, is encoded by the S100A12 gene and a member of the S100 family. Found secreted by neutrophils, monocytes, and keratinocytes in which the protein plays significant roles as antiparasitic, anti-infectious, and antibacterial. Moreover, oligomerization and target interactions by S100A12 is regulated by both zinc and calcium ions resulting in cell signaling and changes in cellular microenvironment.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" } ]

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回收率

[ { "sample": "Serum", "range": "97 - 109 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "113 - 120 %", "average": "= 115" }, { "sample": "Cell culture media", "range": "104 - 116 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "111 - 117 %", "average": "= 114" }, { "sample": "Citrate plasma", "range": "107 - 118 %", "average": "= 113" } ]

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规格

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The S100A12 protein also known as Calgranulin C or CGRP (Calcitonin Gene-Related Peptide) has a molecular mass of approximately 10.4 kDa. This protein shows expression primarily in neutrophils but is also present in other types of cells such as epithelial cells and certain cell lines. S100A12 belongs to the S100 protein family which is part of the EF-hand calcium-binding proteins known for playing roles in cell regulation. The protein binds calcium ions which influence its structural conformation.

Biological function summary

S100A12 is important in the inflammatory response and immune system regulation. It acts as a pro-inflammatory mediator attracting leukocytes to sites of inflammation. Furthermore it can bind to receptors on cell surfaces like RAGE (Receptor for Advanced Glycation End Products) which induces signaling cascades that amplify immune responses. S100A12 does not function alone but often forms homodimers or heterodimers with other S100 proteins enhancing its biological activities.

Pathways

S100A12 participates in pathways like the inflammatory response pathway and the RAGE signaling pathway. By interacting with RAGE and possibly other receptors it impacts cellular responses associated with stress injury and microbial infection. This interaction connects S100A12 to other proteins in the same pathways such as NF-kB an important transcription factor involved in inflammation and immune responses.

S100A12 has connections with conditions like rheumatoid arthritis and inflammatory bowel disease. Elevated levels of S100A12 often correlate with disease activity and severity suggesting its potential as a biomarker. In rheumatoid arthritis it contributes to the inflammatory environment while in inflammatory bowel disease it associates not only with inflammation but also with proteins like IL-6 and TNF-alpha which are significant cytokines in the pathogenesis of these diseases.

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产品实验方案

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靶点信息

S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its pro-inflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of pro-inflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus.

See full target information S100A12

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文献 (2)

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Annals of medicine 57:2451194 PubMed39823189

2025

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Species

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International journal of molecular sciences 23: PubMed35886858

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Species

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Teresa Tsai,Mohannad Alwees,Anika Rost,Janine Theile,H Burkhard Dick,Stephanie C Joachim,Suphi Taneri

View all publications

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websiteProtocolBooklet

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人S100A12/CGRP ELISA试剂盒

Human S100A12/CGRP ELISA Kit

ELISA - Human S100A12/CGRP ELISA Kit (AB282299)

Sandwich ELISA - Human S100A12/CGRP ELISA Kit (AB282299)

Sandwich ELISA - Human S100A12/CGRP ELISA Kit (AB282299)

Sandwich ELISA - Human S100A12/CGRP ELISA Kit (AB282299)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台