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AB324130

Human RNase 7 ELISA Kit

Human RNase 7 ELISA Kit

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Human RNase 7 ELISA Kit is a Sandwich ELISA for the measurement of Human RNase 7 in Human in Cell Culture Media, Biofluids samples.

查看别名

UNQ2516/PRO6006, RNASE7, Ribonuclease 7, RNase 7, Skin-derived antimicrobial protein 2, SAP-2

4 Images
Sandwich ELISA - Human RNase 7 ELISA Kit (AB324130)
  • sELISA

Supplier Data

Sandwich ELISA - Human RNase 7 ELISA Kit (AB324130)

Interpolated concentration of native RNase 7 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human RNase 7 ELISA Kit (AB324130)
  • sELISA

Supplier Data

Sandwich ELISA - Human RNase 7 ELISA Kit (AB324130)

Interpolated concentration of native RNase 7 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : urine 25% and saliva 20%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human RNase 7 ELISA Kit (AB324130)
  • sELISA

Supplier Data

Sandwich ELISA - Human RNase 7 ELISA Kit (AB324130)

Example of human RNase 7 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human RNase 7 ELISA Kit (AB324130)
  • sELISA

Supplier Data

Sandwich ELISA - Human RNase 7 ELISA Kit (AB324130)

Example of human RNase 7 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell culture media, Urine, Saliva

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 2.033 pg/mL

范围

4.375 - 280 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

Assay Kits

AB273299

RNase Activity Assay Kit (Fluorometric)

Functional Studies - RNase Activity Assay Kit (Fluorometric) (AB273299)

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Proteins & Peptides

AB156731

Recombinant Human RNase 7 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human RNase 7 protein (His tag N-Terminus) (AB156731)

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Primary Antibodies

AB205565

Anti-RNase 7 antibody [4C4]

Immunocytochemistry/ Immunofluorescence - Anti-RNase 7 antibody [4C4] (AB205565)

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  • 3
Assay Kits

AB273269

3' to 5' Exonuclease Activity Assay Kit (Fluorometric)

Functional Studies - 3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (AB273269)

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  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human RNase 7 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RNase 7 protein in Cell culture media, Urine, Saliva. Quantitate Human RNase 7 with 2.033 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

  • Singlewash protocol reduces assay time to 90 minutes or less.
  • High sensitivity, specificity and reproducibility from superior antibodies.
  • Fully validated in biological samples.
  • 96wells plate breakable into 12 x 8 wells strips, available in 10pack (10 x 96well plates).
  • Also available in a fully validated 384well format.
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精确度

[ { "reproducibilityType": "Intra", "sample": "Urine", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 10.9" }, { "reproducibilityType": "Inter", "sample": "Urine", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.3" } ]
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回收率

[ { "sample": "Cell culture media", "range": "86 - 115 %", "average": "= 103" }, { "sample": "Urine", "range": "113 - 119 %", "average": "= 117" }, { "sample": "Saliva", "range": "84 - 86 %", "average": "= 85" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Exhibits a potent RNase activity (PubMed : 12244054, PubMed : 12527768, PubMed : 17150966). Has broad-spectrum antimicrobial activity against many pathogenic microorganisms including uropathogenic E.coli (UPEC), and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium (PubMed : 12244054, PubMed : 12527768, PubMed : 17150966, PubMed : 25075772, PubMed : 33818125). Causes loss of bacterial membrane integrity (PubMed : 17150966). Probably contributes to urinary tract sterility (PubMed : 25075772). Bactericidal activity is independent of RNase activity (PubMed : 17150966).
See full target information RNASE7
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websiteProtocolBooklet
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