人Resistin ELISA试剂盒(RETN) (ab108896)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.25 ng/ml - 16 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Resistin ELISA试剂盒(RETN)
参阅全部 Resistin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5.3% 批次间 样品 n Mean SD CV% Overall 10.2% -
样品类型
Cell culture supernatant, Urine, Serum, Plasma, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.2 ng/ml -
范围
0.25 ng/ml - 16 ng/ml -
回收率
= 97 %
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检测时间
5h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Resistin (RETN) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Resistin concentrations in Human plasma, serum, urine, saliva and cell culture supernatants.
A Resistin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Resistin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Resistin captured in plate.
Get results in 90 minutes with Human Resistin ELISA Kit (ab183364) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Resistin Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Resistin Microplate (12 x 8 well strips) 1 unit Resistin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. -
组织特异性
Expressed only in fatty tissues. -
序列相似性
Belongs to the resistin/FIZZ family. -
细胞定位
Secreted. - Information by UniProt
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别名
- Adipose tissue specific secretory factor
- Adipose tissue-specific secretory factor
- ADSF
see all -
数据库链接
- Entrez Gene: 56729 Human
- Omim: 605565 Human
- SwissProt: Q9HD89 Human
- Unigene: 283091 Human
相关产品
-
SimpleStep ELISA kits
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108896于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab108896.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab108896 被引用在 2 文献中.
- Lillyman DJ et al. Axial hypersensitivity is associated with aberrant nerve sprouting in a novel model of disc degeneration in female Sprague Dawley rats. JOR Spine 5:e1212 (2022). PubMed: 36203864
- Al-Rawi N & Al-Marzooq F The Relation between Periodontopathogenic Bacterial Levels and Resistin in the Saliva of Obese Type 2 Diabetic Patients. J Diabetes Res 2017:2643079 (2017). PubMed: 29138754