人PYY ELISA试剂盒(ab255727)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 9.8 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: EDTA Plasma, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人PYY ELISA试剂盒
参阅全部 Peptide YY/PYY 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% extract 8 11.7% 批次间 样品 n Mean SD CV% extract 3 12.2% -
样品类型
Tissue Extracts, EDTA Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
9.8 pg/ml -
范围
31.25 pg/ml - 1000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Tissue Extracts 109 108% - 111% EDTA Plasma 92 81% - 106% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human PYY ELISA Kit (ab255727) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PYY protein in tissue extracts and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PYY with 8.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Peptide PYY, also known as peptide tyrosine tyrosine, is a short 36- amino acid peptide encoded by the gene PYY. PYY is released from L cells in the ileum and colon in response to feeding, and functions by binding to NPY receptors to induce satiety. Two forms of PYY have been identified, PYY1-36 and PYY3-36, with the latter is most common form. PYY is cleaved at the Pro-30 residue by prolyl endopeptidase FAP(Separase) to generate peptide PYY3-36. PYY3-36 binds to the Y2 receptor of the Y family of receptors. PYY inhibits exocrine pancreatic secretion by vasoconstrictor action and inhibits jejunal/colonic mobility, allowing a more efficient digestion and nutrient absorption.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human PYY Capture Antibody 1 x 600µl 10X Human PYY Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human PYY Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. -
序列相似性
Belongs to the NPY family. -
细胞定位
Secreted. - Information by UniProt
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别名
- GHYY
- MGC19143
- Peptide tyrosine tyrosine
see all -
数据库链接
- Entrez Gene: 5697 Human
- Omim: 600781 Human
- SwissProt: P10082 Human
- Unigene: 169249 Human
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab255727于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Interpolated concentrations of native PYY in human small intestine and human colon extract
The concentrations of PYY were measured in duplicate and interpolated from the PYY standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Undiluted samples are as follows: small intestine extract 37.5 μg/mL and colon extract 150 μg/mL.
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Human PYY standard curve exampleThe PYY standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human PYY standard curveThe PYY standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Sandwich ELISA - Human PYY ELISA Kit (ab255727)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - Human PYY ELISA Kit (ab255727)To learn more about the advantages of recombinant antibodies see here. -
Sandwich ELISA - Human PYY ELISA Kit (ab255727)To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab255727 被引用在 1 文献中.
- Ataeinosrat A et al. Effects of Three Different Modes of Resistance Training on Appetite Hormones in Males With Obesity. Front Physiol 13:827335 (2022). PubMed: 35264977