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AB313929

人Pyrophosphatase 1 ELISA试剂盒

Human Pyrophosphatase 1 ELISA Kit

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Human Pyrophosphatase 1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Pyrophosphatase 1 in Human in Cell Culture Media, Biofluids samples.

查看别名

IOPPP, PP, PPA1, Inorganic pyrophosphatase, Pyrophosphate phospho-hydrolase, PPase

1 Images
Sandwich ELISA - Human Pyrophosphatase 1 ELISA Kit (AB313929)
  • sELISA

Supplier Data

Sandwich ELISA - Human Pyrophosphatase 1 ELISA Kit (AB313929)

Example of standard curve using ab313929. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Cell culture supernatant, Plasma, Serum

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 2.46 ng/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human Pyrophosphatase 1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Pyrophosphatase 1 in serum (human Pyrophosphatase 1 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human Pyrophosphatase 1 coated on a 96-well plate.
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回收率

[ { "sample": "Serum", "range": "80 - 96 %", "average": "= 87.28" }, { "sample": "Plasma", "range": "70 - 97 %", "average": "= 79.6" }, { "sample": "Cell culture media", "range": "82 - 99 %", "average": "= 86.41" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313929-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313929-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Pyrophosphatase 1 Antibody-coated ELISA Plate", "number":"AB313929-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Pyrophosphatase 1 Detection Antibody", "number":"AB313929-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB313929-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313929-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313929-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Pyrophosphatase 1 Protein Standard", "number":"AB313929-CMP11", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB313929-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313929-CMP08", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Pyrophosphatase 1 also known as iPPase or inorganic pyrophosphatase 1 is an enzyme responsible for the hydrolysis of inorganic pyrophosphate (PPi) into two phosphate ions. This reaction is energetically favorable and provides the driving force for many biosynthetic processes. Pyrophosphatase 1 is a soluble cytosolic enzyme with a molecular mass of about 33 kDa. It is expressed in various tissues especially high in metabolically active cells like those found in the liver and kidney.
Biological function summary

Pyrophosphate hydrolysis by pyrophosphatase 1 plays a critical role in energy metabolism and phosphate recycling within cells. This enzyme functions independently and is not part of a larger complex. Its activity maintains the balance of phosphate compounds in the cytosol. This balance is important for cellular functions like DNA and RNA synthesis where the removal of PPi is necessary to drive these reactions forward.

Pathways

Pyrophosphatase 1 influences several key metabolic routes such as nucleotide and protein biosynthesis. The enzyme ensures the continuation of these pathways by keeping PPi concentrations low which is important for reactions like aminoacyl-tRNA synthetase activity. Pyrophosphatase 1 connects with enzymes like polymerases and ligases through these pathways facilitating a range of anabolic processes by removing inhibitory PPi byproducts.

The dysregulation of pyrophosphatase 1 can impact conditions like hypophosphatasia and some forms of arthritis. In hypophosphatasia defective function of alkaline phosphatase leads to inadequate pyrophosphate breakdown affecting bone mineralization. Pyrophosphatase 1 by regulating PPi indirectly influences the symptoms when phosphate balance is disrupted. Furthermore its role in phosphate metabolism associates pyrophosphatase 1 with pathways involving matrix proteins that are implicated in mineralization disorders.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information PPA1
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详情请参阅我们的条款与条件。
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