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人蛋白S ELISA试剂盒(ab125969)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 0.22 µg/ml
    • Range: 1 µg/ml - 4 µg/ml
    • Sample type: Cell Lysate, Plasma, Serum, Tissue
    • Detection method: Colorimetric
    • Assay type: Competitive
    • Reacts with: Human

    概述

    • 产品名称

      人蛋白S ELISA试剂盒
      参阅全部 Protein S 试剂盒

    • 检测方法

      Colorimetric

    • 精确度

      批次内
      样品 n Mean SD CV%
      Overall 6.2%
      批次间
      样品 n Mean SD CV%
      Overall 9%

    • 样品类型

      Serum, Plasma, Tissue, Cell Lysate

    • 检测类型

      Competitive

    • 灵敏度

      0.22 µg/ml

    • 范围

      1 µg/ml - 4 µg/ml

    • 回收率

      97 %

    • 检测时间

      4h 00m

    • 实验步骤

      Multiple steps standard assay

    • 种属反应性

      与反应: Human

    • 产品概述

      Abcam’s Protein S Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human protein S in plasma, serum, cell culture supernatants cell lysate and tissue samples.


      A Protein S specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Protein S is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Protein S captured in plate.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • 经测试应用

      适用于: Competitive ELISAmore details

    • 平台

      Microplate

    性能

    • 存放说明

      Store at -20°C. Please refer to protocols.
    • 组件 1 x 96 tests
      100X Streptavidin-Peroxidase Conjugate 1 x 80µl
      10X Diluent N Concentrate 1 x 30ml
      1X Biotinylated Human Protein S (Lyophilized) 1 vial
      20X Wash Buffer Concentrate 1 x 30ml
      Chromogen Substrate 1 x 7ml
      Protein S Microplate (12 x 8 well strips) 1 unit
      Protein S Standard 1 vial
      Sealing Tapes 3 units
      Stop Solution 1 x 11ml

    • 研究领域

    • 功能

      Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.

    • 组织特异性

      Plasma.

    • 疾病相关

      Defects in PROS1 are the cause of protein S deficiency (PROS1D) [MIM:612336]; also known as thrombophilia due to protein S deficiency. PROS1D is a cause of hereditary thrombophilia, a hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Based on the plasma levels of total and free PROS1 antigen as well as the serine protease-activated protein C cofactor activity, three types of PROS1D have been described: type I, characterized by reduced total and free PROS1 antigen levels together with reduced anticoagulant activity; type III, in which only free PROS1 antigen and PROS1 activity levels are reduced; and the rare type II which is characterized by normal concentrations of both total and free PROS1 antigen, but low cofactor activity.

    • 序列相似性

      Contains 4 EGF-like domains.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 2 laminin G-like domains.

    • 翻译后修饰

      The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

    • 细胞定位

      Secreted.
    • Information by UniProt

    • 别名

      • Preproprotein S
      • Propiece of latent protein S
      • PROS
      • PROS 1
      • PROS_HUMAN
      • proS1
      • Protein S alpha
      • Protein Sa
      • PS 21
      • PS 22
      • PS 23
      • PS 24
      • PS 25
      • PS 26
      • PS21
      • PS22
      • PS23
      • PS24
      • PS25
      • PS26
      • PSA
      • THPH5
      • THPH6
      • Vitamin K dependent protein S
      • Vitamin K-dependent plasma protein S
      • Vitamin K-dependent protein S
      see all

    • 数据库链接

      应用

      The Abpromise guarantee

      Abpromise™承诺保证使用ab125969于以下的经测试应用

      “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

      应用 Ab评论 说明
      Competitive ELISA
      Use at an assay dependent concentration.
      说明
      Competitive ELISA
      Use at an assay dependent concentration.

      图片

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab125969

      文献 (1)

      发表研究结果有使用 ab125969？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab125969 被引用在 1 文献中.

      • Liang Y  et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). PubMed: 29599912

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