人Progesterone ELISA试剂盒(ab108670)
Key features and details
- Sensitivity: 0.05 ng/ml
- Range: 0.2 ng/ml - 40 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
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产品名称
人Progesterone ELISA试剂盒 -
检测方法
Colorimetric -
样品类型
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Competitive -
灵敏度
0.05 ng/ml -
范围
0.2 ng/ml - 40 ng/ml -
回收率
106 %
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实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human
预测可用于: Mammals
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产品概述
Abcam’s Progesterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Progesterone in serum and plasma.
A 96-well plate has been precoated with anti-Progesterone. Samples and the Progesterone-HRP conjugate are added to the wells, where Progesterone in the sample competes with the added Progesterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Progesterone in the sample and the intensity is measured at 450 nm.
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经测试应用
适用于: Competitive ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 10X Wash Solution 1 x 50ml Anti-Progesterone IgG Coated Wells 1 unit Cover foils 1 unit Progesterone control 1 x 1ml Progesterone Standard 0 - 0 ng/mL 1 x 1ml Progesterone Standard 1 - 0.2 ng/mL 1 x 1ml Progesterone Standard 2 - 1.0 ng/mL 1 x 1ml Progesterone Standard 3 - 5.0 ng/mL 1 x 1ml Progesterone Standard 4 - 15.0 ng/mL 1 x 1ml Progesterone Standard 5 - 40.0 ng/mL 1 x 1ml Progesterone-HRP Conjugate 1 x 22ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108670于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative Standard Curve using ab108670.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab108670 被引用在 11 文献中.
- Rowland SN et al. Trimethylamine N-Oxide Concentration and Blood Pressure in Young Healthy Men and Women: A Replicated Crossover Study. Metabolites 13:N/A (2023). PubMed: 37512583
- Cheng J et al. Effects of hypoxia-preconditioned HucMSCs on neovascularization and follicle survival in frozen/thawed human ovarian cortex transplanted to immunodeficient mice. Stem Cell Res Ther 13:474 (2022). PubMed: 36104746
- Nankam PAN et al. Is subcutaneous adipose tissue expansion in people living with lipedema healthier and reflected by circulating parameters? Front Endocrinol (Lausanne) 13:1000094 (2022). PubMed: 36387874
- Liu Y et al. Expression of SUMO associated proteins in the mouse endometrium is regulated by ovarian hormones throughout the estrous cycle. Exp Ther Med 19:1855-1863 (2020). ELISA . PubMed: 32104241
- Sun T et al. Differences in First-Trimester Maternal Metabolomic Profiles in Pregnancies Conceived From Fertility Treatments. J Clin Endocrinol Metab 104:1005-1019 (2019). PubMed: 30445606