Human PLGF ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PLGF with a sensitivity of 4.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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PGFL, PLGF, PGF, Placenta growth factor, PlGF
- sELISA
Lab
Sandwich ELISA - Human PLGF ELISA Kit (AB260056)
Serum from ten individual healthy human male donors was measured in duplicate
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PIGF concentration was determined to be 38.42 pg/mL in detectable samples with a range of ND - 44.05 pg/mL for all samples.
- sELISA
Lab
Sandwich ELISA - Human PLGF ELISA Kit (AB260056)
Interpolated concentrations of native PLGF in human pregnant serum and JAR cell culture supernatant samples
The concentrations of PIGF were measured in duplicates, interpolated from the PIGF standard curves and corrected for sample dilution. Undiluted samples are as follows : pregnant serum 50% and JAR cell culture supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PIGF concentration was determined to be 1,147 pg/mL in neat pregnant serum and 11,000 pg/mL in neat JAR cell culture supernatant.
- sELISA
Lab
Sandwich ELISA - Human PLGF ELISA Kit (AB260056)
Example of human PLGF standard curve in Sample Diluent NS prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Human PLGF ELISA Kit (ab260056) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PLGF protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PLGF with 4.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
PLGF (placenta growth factor) is a secreted growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. Four PLGF isoforms are produced by alternative splicing, three of them are present in placental tissue. Only PLGF-1 is found in the colon and mammary carcinomas. PLGF-3 binds to the receptor FLT1/VEGFR-1. Isoform PLGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.
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Scientific reports 14:24117 PubMed39406891
2024
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