人Periostin ELISA试剂盒
Human Periostin ELISA Kit
- Recombinant
- SimpleStep
- 了解详情
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Human Periostin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Periostin with a sensitivity of 20.98 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
查看别名
OSF2, POSTN, Periostin, PN, Osteoblast-specific factor 2, OSF-2
- sELISA
Supplier Data
Sandwich ELISA - Human Periostin ELISA Kit (AB309314)
Interpolated concentration of native Periostin was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 80 serum, plasma (citrate), plasma (heparin), and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human Periostin ELISA Kit (AB309314)
Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Periostin concentration was determined to be 123.81 ng/mL with a range of 47.55 – 200.71 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Periostin ELISA Kit (AB309314)
Example of human Periostin standard curve. Background-subtracted data values (mean +/- SD) are graphed.
反应性数据
产品详情
Human Periostin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Periostin protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Periostin with 20.98 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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性能和储存信息
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储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The periostin protein contributes to the maintenance of tissue integrity and mechanical properties. It does not solely act; it forms part of a multiprotein complex with collagen and other extracellular matrix components. This involvement is necessary for maintaining cellular interactions and promoting tissue healing processes. Additionally periostin influences cell proliferation and migration impacting developmental and wound healing biology.
Pathways
Periostin is involved in the integrin signaling pathway and the Wnt/β-catenin pathway both important for cell communication and movement. Through these pathways periostin interacts with integrins αvβ3 and αvβ5 facilitating cell adhesion and migration. The Wnt/β-catenin pathway interaction involves several proteins including Frizzled receptors ensuring regulation of cellular proliferation and differentiation. These pathways make periostin a coordinator of complex cellular processes.
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