AB234568

人PDGF-CC ELISA试剂盒

Human PDGF-CC ELISA Kit

Recombinant
SimpleStep
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(2 Publications)

Human PDGF-CC ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PDGF-CC with a sensitivity of 2.79 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

SCDGF, UNQ174/PRO200, PDGFC, Platelet-derived growth factor C, PDGF-C, Fallotein, Spinal cord-derived growth factor, VEGF-E

6 Images

Sandwich ELISA - Human PDGF-CC ELISA Kit (AB234568)

sELISA

Supplier Data

Sandwich ELISA - Human PDGF-CC ELISA Kit (AB234568)

Interpolated concentrations of spiked PDGF-CC in human serum and plasma samples.

The concentrations of PDGF-CC were measured in duplicates, interpolated from the PDGF-CC standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25% and platelet poor plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGF-CC concentration was determined to be 1501.39 pg/mL in serum and 864.88 pg/mL in platelet poor plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Human PDGF-CC ELISA Kit (AB234568)

Serum from ten individual healthy human female donors were measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGF-CC concentration was determined to be 91.69 pg/mL with a range of 11.83 - 446.58 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human PDGF-CC ELISA Kit (AB234568)

Human PDGF-CC Competitor Std Curve Comparison.

Standard curve comparison between human PDGF-CC SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human PDGF-CC ELISA Kit (AB234568)

Interpolated concentrations of native PDGF-CC in human OVACR-3 supernatant 100%.

The concentrations of PDGF-CC were measured in duplicate and interpolated from the PDGF-CC standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGF-CC concentration was determined to be 651.77 pg/mL in OVACR-3 supernatant 100%.

sELISA

Supplier Data

Sandwich ELISA - Human PDGF-CC ELISA Kit (AB234568)

Serum from ten individual healthy human male donors were measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGF-CC concentration was determined to be 37.47 pg/mL with a range of 18.78 - 66.17 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human PDGF-CC ELISA Kit (AB234568)

Example of human PDGF-CC standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Citrate plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich

灵敏度

= 2.79 pg/mL

范围

28.125 - 1800 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human PDGF-CC ELISA Kit (ab234568) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PDGF-CC protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PDGF-CC with 2.79 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" } ]

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回收率

[ { "sample": "Cell culture supernatant", "range": "91 - 106 %", "average": "= 98" }, { "sample": "Serum", "range": "78 - 86 %", "average": "= 82" }, { "sample": "Citrate plasma", "range": "85 - 89 %", "average": "= 87" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab234568-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB234568-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB234568-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB234568-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB234568-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PDGF-CC Lyophilized Recombinant Protein", "number":"AB234568-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PDGF-CC Capture Antibody", "number":"AB234568-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PDGF-CC Detector Antibody", "number":"AB234568-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB234568-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB234568-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB234568-CMP06", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Platelet-Derived Growth Factor CC (PDGF-CC) is a member of the PDGF family consisting of homodimeric and heterodimeric isoforms. PDGF-CC with a mass of approximately 44 kDa partakes in various cellular processes. It primarily expresses in fibroblasts endothelial cells and some types of neurons. PDGF-CC functions by binding to tyrosine kinase receptors specifically PDGFRαα and PDGFRαβ where it triggers intracellular signaling pathways.

Biological function summary

PDGF-CC influences cell proliferation migration and survival particularly in the context of vascular development and remodeling. It acts as a powerful growth factor for mesenchymal cells and plays a role in embryonic development. PDGF-CC forms a functional complex after proteolytic activation which is essential for its receptor-binding capacity. This factor significantly regulates angiogenesis and wound healing processes.

Pathways

PDGF-CC is integral to the PDGF signaling pathway and is involved in the PI3K/AKT signaling pathway. These pathways promote cellular growth survival and proliferation. PDGF-CC's interaction with PDGF receptors primarily PDGFRα facilitates these biological functions. In these pathways PDGF-CC interacts with other proteins such as PI3K and AKT connecting it to broader cellular response networks.

PDGF-CC has been associated with fibrotic diseases and certain cancers. Its overexpression or dysregulation can contribute to pathological fibrosis through excessive tissue repair. PDGF-CC is also linked to glioblastoma a type of brain cancer where abnormal PDGF signaling leads to tumor progression. PDGF-CC interacts with PDGFRα in these disease contexts highlighting its contribution to disease mechanisms when dysregulated.

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产品实验方案

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靶点信息

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages : inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function.

See full target information PDGFC

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 22:1023 PubMed39543636

2024

Targeting PDGF-CC as a promising therapeutic strategy to inhibit cholangiocarcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenchao Luo,Fangfang Zhou,Canliang Tan,Liangchun Yin,Man Bao,Xiang He,Haohui Li,Jian Yan

Cancer gene therapy 28:126-140 PubMed32616906

2020

MicroRNA-375 represses tumor angiogenesis and reverses resistance to sorafenib in hepatocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Li,Tao Wang,Fei-Fan Sun,Jian-Qiong Feng,Jing-Jing Peng,Hua Li,Chao Wang,Dan Wang,Yu Liu,Yu-Di Bai,Mao-Lin Shi,Tao Zhang

View all publications

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websiteProtocolBooklet

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人PDGF-CC ELISA试剂盒

Human PDGF-CC ELISA Kit

Sandwich ELISA - Human PDGF-CC ELISA Kit (AB234568)

Sandwich ELISA - Human PDGF-CC ELISA Kit (AB234568)

Sandwich ELISA - Human PDGF-CC ELISA Kit (AB234568)

Sandwich ELISA - Human PDGF-CC ELISA Kit (AB234568)

Sandwich ELISA - Human PDGF-CC ELISA Kit (AB234568)

Sandwich ELISA - Human PDGF-CC ELISA Kit (AB234568)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台