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AB212159

人Parkin ELISA试剂盒

Human Parkin ELISA Kit

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Human Parkin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Parkin with a sensitivity of 9.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

PARK2, PRKN, E3 ubiquitin-protein ligase parkin, Parkin, Parkin RBR E3 ubiquitin-protein ligase, Parkinson juvenile disease protein 2, Parkinson disease protein 2

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ELISA - Human Parkin ELISA Kit (AB212159)
  • ELISA

Supplier Data

ELISA - Human Parkin ELISA Kit (AB212159)
Sandwich ELISA - Human Parkin ELISA Kit (AB212159)
  • sELISA

Unknown

Sandwich ELISA - Human Parkin ELISA Kit (AB212159)

Interpolated concentrations of Parkin in extract of HEK293T cells overexpressing Parkin samples based on a 2.5 μg/mL extract load.

The concentrations of Parkin were measured in duplicate and interpolated from the Parkin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Parkin concentration was determined to be 2,180 pg/mL in Parkin overexpression extract.

Sandwich ELISA - Human Parkin ELISA Kit (AB212159)
  • sELISA

Supplier Data

Sandwich ELISA - Human Parkin ELISA Kit (AB212159)

Representative image of Human Parkin ELISA Kit (ab212159)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Parkin Capture Antibody

- 10X Human Parkin Detector Antibody

- Human Parkin Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human Parkin ELISA Kit (AB212159)
  • sELISA

Supplier Data

Sandwich ELISA - Human Parkin ELISA Kit (AB212159)

Example of human Parkin standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Parkin ELISA Kit (AB212159)
  • sELISA

Unknown

Sandwich ELISA - Human Parkin ELISA Kit (AB212159)

Interpolated concentrations of Parkin in extracts of HEK293T cells overexpressing Parkin and HEK293T cells transfected with the associated empty vector.

The concentrations of Parkin were measured in three different dilutions in duplicate and interpolated from the Parkin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng Parkin per mg of extract (mean +/- SD, n=3). Parkin concentration was determined to be 830 ng/mg in extract of HEK293T cells overexpressing Parkin, and 1 ng/mg in extract of HEK293T cells transfected with the empty vector.

关键信息

检测方法

Colorimetric

样品类型

Cell culture extracts, Tissue Extracts

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 9.7 pg/mL

范围

46.88 - 3000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human Parkin ELISA Kit ab212159 is a rapid single-wash 90-min Sandwich ELISA to measure Human Parkin in cell culture extracts, tissue extracts. This SimpleStep sensitivity is 9.7 pg/mL.

How the assay works

Human Parkin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Parkin ELISA Kit ab212159 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Parkin antibody [EPR18567-176] - BSA and Azide free (Capture) ab242818
- Anti-Parkin antibody [EPR18567-214] - BSA and Azide free (Detector) ab242818
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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]
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回收率

[ { "sample": "Cell culture extracts", "range": "103 - 108 %", "average": "= 106" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab212159-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB212159-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB212159-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB212159-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB212159-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB212159-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB212159-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB212159-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Parkin Capture Antibody", "number":"AB212159-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Parkin Lyophilized Recombinant Protein", "number":"AB212159-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Parkin Detector Antibody", "number":"AB212159-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB212159-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB212159-CMP09", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Parkin protein also known as PRK8 or Park2 is an E3 ubiquitin ligase with a molecular weight of approximately 52 kDa. This protein plays a critical role in tagging damaged proteins for degradation maintaining cellular health. Parkin is expressed in various tissues with significant levels in dopaminergic neurons in the brain. It is encoded by the PARK2 gene and has been linked to the regulation of mitochondrial quality and autophagy processes contributing to cellular homeostasis.
Biological function summary

Parkin is essential for the regulation of mitochondria through its involvement in the mitochondrial quality control system. It functions as part of a complex with other proteins that respond to mitochondrial damage by tagging them with ubiquitin molecules. This mechanism allows for the removal of defective mitochondria via mitophagy critical for preventing the accumulation of damaged cellular components.

Pathways

Parkin interacts with pathways involved in the cellular stress response particularly the PINK1 (PTEN Induced Kinase

  1. pathway. PINK1 phosphorylates Parkin activating it to label damaged mitochondria. Another critical pathway involves proteasomal degradation where Parkin collaborates with Ubiquitin to manage protein turnover. These pathways highlight its relationships with other cellular stress-regulating proteins enhancing our understanding of its roles in maintaining cellular integrity.
Mutations in the gene coding for Parkin are linked to Parkinson's disease (PD) and some forms of juvenile autosomal recessive parkinsonism. The Parkin protein's dysfunctional activity leads to impaired mitochondrial management and protein aggregation in neurons contributing significantly to neurodegenerative disease. In conditions such as PD Parkin interacts with other proteins such as PINK1 reinforcing its role in mitochondrial protection and indicating the protein's importance in disease progression and potential therapeutic targeting.
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产品实验方案

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靶点信息

Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (PubMed : 10888878, PubMed : 10973942, PubMed : 11431533, PubMed : 12150907, PubMed : 12628165, PubMed : 15105460, PubMed : 16135753, PubMed : 21376232, PubMed : 21532592, PubMed : 22396657, PubMed : 23620051, PubMed : 23754282, PubMed : 24660806, PubMed : 24751536, PubMed : 29311685, PubMed : 32047033). Substrates include SYT11 and VDAC1 (PubMed : 29311685, PubMed : 32047033). Other substrates are BCL2, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPTIN5, TOMM20, USP30, ZNF746, MIRO1 and AIMP2 (PubMed : 10888878, PubMed : 10973942, PubMed : 11431533, PubMed : 12150907, PubMed : 12628165, PubMed : 15105460, PubMed : 16135753, PubMed : 21376232, PubMed : 21532592, PubMed : 22396657, PubMed : 23620051, PubMed : 23754282, PubMed : 24660806, PubMed : 24751536). Mediates monoubiquitination as well as 'Lys-6', 'Lys-11', 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context (PubMed : 19229105, PubMed : 20889974, PubMed : 25474007, PubMed : 25621951, PubMed : 32047033). Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7 : 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation (PubMed : 17846173, PubMed : 19229105). Mediates 'Lys-63'-linked polyubiquitination of a 22 kDa O-linked glycosylated isoform of SNCAIP, possibly playing a role in Lewy-body formation (PubMed : 11431533, PubMed : 11590439, PubMed : 15105460, PubMed : 15728840, PubMed : 19229105). Mediates monoubiquitination of BCL2, thereby acting as a positive regulator of autophagy (PubMed : 20889974). Protects against mitochondrial dysfunction during cellular stress, by acting downstream of PINK1 to coordinate mitochondrial quality control mechanisms that remove and replace dysfunctional mitochondrial components (PubMed : 11439185, PubMed : 18957282, PubMed : 19029340, PubMed : 19966284, PubMed : 21376232, PubMed : 22082830, PubMed : 22396657, PubMed : 23620051, PubMed : 23933751, PubMed : 24660806, PubMed : 24784582, PubMed : 24896179, PubMed : 25474007, PubMed : 25527291, PubMed : 32047033). Depending on the severity of mitochondrial damage and/or dysfunction, activity ranges from preventing apoptosis and stimulating mitochondrial biogenesis to regulating mitochondrial dynamics and eliminating severely damaged mitochondria via mitophagy (PubMed : 11439185, PubMed : 19029340, PubMed : 19801972, PubMed : 19966284, PubMed : 21376232, PubMed : 22082830, PubMed : 22396657, PubMed : 23620051, PubMed : 23685073, PubMed : 23933751, PubMed : 24896179, PubMed : 25527291, PubMed : 32047033, PubMed : 33499712). Activation and recruitment onto the outer membrane of damaged/dysfunctional mitochondria (OMM) requires PINK1-mediated phosphorylation of both PRKN and ubiquitin (PubMed : 24660806, PubMed : 24784582, PubMed : 25474007, PubMed : 25527291). After mitochondrial damage, functions with PINK1 to mediate the decision between mitophagy or preventing apoptosis by inducing either the poly- or monoubiquitination of VDAC1, respectively; polyubiquitination of VDAC1 promotes mitophagy, while monoubiquitination of VDAC1 decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed : 27534820, PubMed : 32047033). When cellular stress results in irreversible mitochondrial damage, promotes the autophagic degradation of dysfunctional depolarized mitochondria (mitophagy) by promoting the ubiquitination of mitochondrial proteins such as TOMM20, RHOT1/MIRO1, MFN1 and USP30 (PubMed : 19029340, PubMed : 19966284, PubMed : 21753002, PubMed : 22396657, PubMed : 23620051, PubMed : 23685073, PubMed : 23933751, PubMed : 24896179, PubMed : 25527291). Preferentially assembles 'Lys-6'-, 'Lys-11'- and 'Lys-63'-linked polyubiquitin chains, leading to mitophagy (PubMed : 25621951, PubMed : 32047033). The PINK1-PRKN pathway also promotes fission of damaged mitochondria by PINK1-mediated phosphorylation which promotes the PRKN-dependent degradation of mitochondrial proteins involved in fission such as MFN2 (PubMed : 23620051). This prevents the refusion of unhealthy mitochondria with the mitochondrial network or initiates mitochondrial fragmentation facilitating their later engulfment by autophagosomes (PubMed : 23620051). Regulates motility of damaged mitochondria via the ubiquitination and subsequent degradation of MIRO1 and MIRO2; in motor neurons, this likely inhibits mitochondrial intracellular anterograde transport along the axons which probably increases the chance of the mitochondria undergoing mitophagy in the soma (PubMed : 22396657). Involved in mitochondrial biogenesis via the 'Lys-48'-linked polyubiquitination of transcriptional repressor ZNF746/PARIS which leads to its subsequent proteasomal degradation and allows activation of the transcription factor PPARGC1A (PubMed : 21376232). Limits the production of reactive oxygen species (ROS) (PubMed : 18541373). Regulates cyclin-E during neuronal apoptosis (PubMed : 12628165). In collaboration with CHPF isoform 2, may enhance cell viability and protect cells from oxidative stress (PubMed : 22082830). Independently of its ubiquitin ligase activity, protects from apoptosis by the transcriptional repression of p53/TP53 (PubMed : 19801972). May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity (PubMed : 11439185). May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. May represent a tumor suppressor gene (PubMed : 12719539).
See full target information PRKN
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 8:642749 PubMed34055831

2021

Prognostic Significance of the Fission1/Parkin Ratio for Sepsis: A Prospective Cohort Study.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Huang,Xiaoting Wang,Hongmin Zhang,Guangjian Wang,Dawei Liu
View all publications
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