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AB277440

人PAR1/Thrombin Receptor ELISA试剂盒

Human PAR1/Thrombin Receptor ELISA Kit

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Human PAR1/Thrombin Receptor ELISA Kit is a Sandwich ELISA for the measurement of Human PAR1/Thrombin Receptor in Human in Biofluids, Cell Culture Media samples.

查看别名

CF2R, PAR1, TR, F2R, Proteinase-activated receptor 1, PAR-1, Coagulation factor II receptor, Thrombin receptor

1 Images
ELISA - Human PAR1/Thrombin Receptor ELISA Kit (AB277440)
  • ELISA

Supplier Data

ELISA - Human PAR1/Thrombin Receptor ELISA Kit (AB277440)

These standard curves are for demonstration only. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 3 pg/mL

范围

2.87 - 700 pg/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

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Thrombin Activity Assay Kit (Fluorometric)

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Sandwich ELISA - Human Thrombin-Antithrombin Complex ELISA Kit (TAT) (AB108907)

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Human Fibrinogen ELISA Kit - high sensitivity

Recombinant|SimpleStep

ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

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Assay Kits

AB137976

Human Factor V ELISA Kit

Sandwich ELISA - Human Factor V ELISA Kit (AB137976)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human PAR1/Thrombin Receptor ELISA Kit (ab277440) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PAR1/Thrombin Receptor in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human PAR1/Thrombin Receptor coated on a 96-well plate. Standards and samples are pipetted into the wells and Human PAR1/Thrombin Receptor present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human PAR1/Thrombin Receptor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human PAR1/Thrombin Receptor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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回收率

[ { "sample": "Serum", "range": "100 - 110 %", "average": "= 105.3" }, { "sample": "Plasma", "range": "103 - 125 %", "average": "= 113.2" }, { "sample": "Cell culture media", "range": "115 - 133 %", "average": "= 123" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277440-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB277440-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277440-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277440-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human PAR1 Thrombin Receptor coated Microplate", "number":"AB277440-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277440-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277440-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PAR1/Thrombin Receptor standard protein (Lyophilized)", "number":"AB277440-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human PAR1/Thrombin Receptor Antibody", "number":"AB277440-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB277440-CMP03", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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产品实验方案

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靶点信息

High affinity receptor that binds the activated thrombin, leading to calcium release from intracellular stores (PubMed : 1672265, PubMed : 8136362). The thrombin-activated receptor signaling pathway is mediated through PTX-insensitive G proteins, activation of phospholipase C resulting in the production of 1D-myo-inositol 1,4,5-trisphosphate (InsP3) which binds to InsP3 receptors causing calcium release from the stores (By similarity). In astrocytes, the calcium released into the cytosol allows the Ca(2+)-dependent release of L-glutamate into the synaptic cleft through BEST1, that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation (By similarity). May play a role in platelets activation and in vascular development (PubMed : 10079109). Mediates up-regulation of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, triggered by coagulation factor Xa (F10) in cardiac fibroblasts and umbilical vein endothelial cells (PubMed : 30568593, PubMed : 34831181).
See full target information F2R
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en
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