AB137969

人Pancreatic Amylase ELISA试剂盒

Name: 人Pancreatic Amylase ELISA试剂盒 (ab137969)| Abcam中文官网
Brand: Abcam Limited
SKU: AB137969
Price: 7527 CNY
Availability: InStock

Human Pancreatic Amylase ELISA Kit

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(7 Publications)

Human Pancreatic Amylase ELISA Kit is a Sandwich ELISA for the measurement of Human Pancreatic Amylase in Human in Biofluids samples.

查看别名

Pancreatic alpha-amylase, PA, AMY2A

1 Images

Sandwich ELISA - Human Pancreatic Amylase ELISA Kit (AB137969)

sELISA

Supplier Data

Sandwich ELISA - Human Pancreatic Amylase ELISA Kit (AB137969)

Representative Standard Curve using ab137969

关键信息

检测方法

Colorimetric

样品类型

Saliva, Urine, Plasma, Milk, Serum

反应种属

Human

检测类型

Sandwich

灵敏度

> 0.053 mIU/mL

范围

0.156 - 10 mIU/mL

检测时间

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Abcam's Pancreatic Amylase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human pancreatic amylase in plasma, serum, urine, milk, saliva samples.

A Pancreatic Amylase specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Pancreatic Amylase specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Pancreatic Amylase captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.3" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4" } ]

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规格

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性能和储存信息

运输条件

Blue Ice

储存信息

Please refer to protocols

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Pancreatic amylase also known as alpha-amylase or amylopsin is an enzyme with a molecular mass of approximately 55 kDa. This enzyme is expressed mainly in the pancreas and is secreted into the small intestine. Its primary mechanical function involves hydrolyzing starch into smaller carbohydrates such as maltose and dextrin. Pancreatic amylase activity is essential for the efficient digestion of dietary starch aiding in the breakdown into sugars that the body can easily absorb and utilize.

Biological function summary

The enzyme facilitates the digestive process by catalyzing the cleavage of 14-glycosidic bonds in polysaccharides. Pancreatic amylase operates independently and not as part of a larger protein complex. Its function exclusively converts complex carbohydrates into simpler absorbable sugars. This role is essential in human metabolism as it initiates the conversion of dietary carbohydrates into forms that the intestine can further process and transport throughout the body.

Pathways

Pancreatic amylase participates in the carbohydrate digestion and absorption pathway. It interacts with other enzymes such as maltase and isomaltase which further break down the products of its enzymatic activity. This enzyme plays a core role in the pathway ensuring proper starch conversion into glucose which cells can uptake for energy. Pancreatic amylase parallels salivary amylase which begins the starch digestion process in the mouth.

Pancreatic amylase levels serve as a marker for pancreatitis an inflammation of the pancreas. Elevated serum levels indicate pancreatic injury or inflammation pointing to disorders that affect enzyme secretion. Chronic pancreatitis or acute pancreatitis often correlates with increased pancreatic amylase. Furthermore in certain digestive disorders like cystic fibrosis the secretion of pancreatic amylase may be disrupted indicating its pivotal role in maintaining pancreatic function and health. The enzyme's activity measurement can aid in diagnosing these conditions and monitoring their progression.

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产品实验方案

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靶点信息

See full target information Pancreatic alpha-amylase

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of oral & facial pain and headache 38:111-118 PubMed39801101

2025

Changes in salivary biomarkers of burning mouth syndrome patients after clonazepam treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Sungil Jang,Ji-Eun Kim,Young-Hee Lee,Won Jung

Heliyon 10:e32828 PubMed38975221

2024

Investigation of the human-gut-kidney axis by fecal proteomics, highlights molecular mechanisms affected in CKD.

Applications

Unspecified application

Species

Unspecified reactive species

Sonnal Lohia,Sophie Valkenburg,Rafael Stroggilos,Vasiliki Lygirou,Manousos Makridakis,Jerome Zoidakis,Francis Verbeke,Griet Glorieux,Antonia Vlahou

Cell reports 43:114346 PubMed38850534

2024

Impaired islet function and normal exocrine enzyme secretion occur with low inter-regional variation in type 1 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Denise M Drotar,Ana Karen Mojica-Avila,Drew T Bloss,Christian M Cohrs,Cameron T Manson,Amanda L Posgai,MacKenzie D Williams,Maigan A Brusko,Edward A Phelps,Clive H Wasserfall,Stephan Speier,Mark A Atkinson

Infection and drug resistance 17:2077-2088 PubMed38813526

2024

Microbiological Profile of Patients with Aspiration Pneumonia Identified by Combined Detection Methods.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Xu,Ruixue Zhang,Xiaoxue Zhang,Yueguang Cheng,Liping Lv,Lianjun Lin

Medical principles and practice : international journal of the Kuwait University, Health Science Centre 33:260-268 PubMed38479367

2024

The Modulation of Euglycemic Endocrine and Exocrine Pancreatic Secretions in Iron Deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad A Bani-Ahmad,Noor E Abu Tayyem

Nature microbiology 8:1641-1652 PubMed37563289

2023

Bacterial amylases enable glycogen degradation by the vaginal microbiome.

Applications

Unspecified application

Species

Unspecified reactive species

Dominick J Jenkins,Benjamin M Woolston,M Indriati Hood-Pishchany,Paula Pelayo,Alyssa N Konopaski,M Quinn Peters,Michael T France,Jacques Ravel,Caroline M Mitchell,Seth Rakoff-Nahoum,Christopher Whidbey,Emily P Balskus

Cell death & disease 13:863 PubMed36220838

2022

Neddylation-mediated degradation of hnRNPA2B1 contributes to hypertriglyceridemia pancreatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Chen,Yilong Wang,Wenwen Xia,Jinbao Zhang,Yan Zhao

View all publications

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websiteProtocolBooklet

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人Pancreatic Amylase ELISA试剂盒

Human Pancreatic Amylase ELISA Kit

Sandwich ELISA - Human Pancreatic Amylase ELISA Kit (AB137969)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台