人PAI1 ELISA试剂盒(SERPINE1) (ab108891)
Key features and details
- Sensitivity: 19 pg/ml
- Range: 0.15 ng/ml - 1.25 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Cerebral Spinal Fluid, Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人PAI1 ELISA试剂盒(SERPINE1)
参阅全部 PAI1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 3.9% 批次间 样品 n Mean SD CV% Overall 8.4% -
样品类型
Cell culture supernatant, Serum, Plasma, Tissue, Cell Lysate, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
= 19 pg/ml -
范围
0.15 ng/ml - 1.25 ng/ml -
回收率
98 %
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检测时间
4h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s PAI1 (SERPINE1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 concentrations in Human plasma, serum, tissue extracts and cell culture supernatants.
A PAI1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 captured in plate.
This assay recognizes both natural and recombinant Human PAI-1.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human PAI1 Antibody 1 x 120µl Chromogen Substrate 1 x 7ml PAI1 Microplate (12 x 8 well strips) 1 unit PAI1 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. -
组织特异性
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells. -
疾病相关
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. -
序列相似性
Belongs to the serpin family. -
翻译后修饰
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond. -
细胞定位
Secreted. - Information by UniProt
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别名
- Clade E
- Endothelial plasminogen activator inhibitor
- Nexin
see all -
数据库链接
- Entrez Gene: 5054 Human
- Omim: 173360 Human
- SwissProt: P05121 Human
- Unigene: 414795 Human
- Unigene: 713079 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108891于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Sandwich ELISA - 108891 PAI1 (SERPINE1) Human ELISA Kit
Standard curve mean of duplicates (+/- SD) with background readings subtracted
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Sandwich ELISA - 108891 PAI1 (SERPINE1) Human ELISA KitPAI1 measured in various samples showing quantity (ng) per mL of tested sample
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Sandwich ELISA - 108891 PAI1 (SERPINE1) Human ELISA KitPAI1 measured in cell lysates showing quantity (ng) per 1 million cells tested
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab108891 被引用在 10 文献中.
- Kelliher S et al. Non-severe COVID-19 is associated with endothelial damage and hypercoagulability despite pharmacological thromboprophylaxis. J Thromb Haemost 20:1008-1014 (2022). PubMed: 35102689
- Rosendal E et al. Serine Protease Inhibitors Restrict Host Susceptibility to SARS-CoV-2 Infections. mBio 13:e0089222 (2022). PubMed: 35532162
- Rodríguez-Lorenzo S et al. Altered secretory and neuroprotective function of the choroid plexus in progressive multiple sclerosis. Acta Neuropathol Commun 8:35 (2020). PubMed: 32192527
- Li B et al. MicroRNA-30b protects myocardial cell function in patients with acute myocardial ischemia by targeting plasminogen activator inhibitor-1. Exp Ther Med 15:5125-5132 (2018). PubMed: 29805539
- Arnandis T et al. Oxidative Stress in Cells with Extra Centrosomes Drives Non-Cell-Autonomous Invasion. Dev Cell 47:409-424.e9 (2018). PubMed: 30458137
- Al-Awadhi FH et al. Grassystatins D-F, Potent Aspartic Protease Inhibitors from Marine Cyanobacteria as Potential Antimetastatic Agents Targeting Invasive Breast Cancer. J Nat Prod 80:2969-2986 (2017). PubMed: 29087712
- Abell SK et al. The association between dysregulated adipocytokines in early pregnancy and development of gestational diabetes. Diabetes Metab Res Rev 33:N/A (2017). PubMed: 28806491
- Li X et al. Regulatory role of microRNA-30b and plasminogen activator inhibitor-1 in the pathogenesis of cognitive impairment. Exp Ther Med 11:1993-1998 (2016). PubMed: 27168840
- Giacoia EG et al. PAI-1 leads to G1-phase cell-cycle progression through cyclin D3/cdk4/6 upregulation. Mol Cancer Res 12:322-34 (2014). PubMed: 24464915
- Jin R et al. Dietary fructose reduction improves markers of cardiovascular disease risk in Hispanic-American adolescents with NAFLD. Nutrients 6:3187-201 (2014). Human . PubMed: 25111123