人PAEP ELISA试剂盒(Glycodelin) (ab275904)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3 pg/ml
- Range: 7.81 pg/ml - 500 pg/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人PAEP ELISA试剂盒(Glycodelin)
参阅全部 PAEP/Glycodelin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 8 5.2% 批次间 样品 n Mean SD CV% Serum 3 7.98% -
样品类型
Serum -
检测类型
Sandwich (quantitative) -
灵敏度
3 pg/ml -
范围
7.81 pg/ml - 500 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 107 89% - 116% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human PAEP ELISA kit (ab275904) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PAEP protein in human serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PAEP with 3 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
PAEP, progestogen-associated endometrial protein also known as glycodelin, is a glycoprotein which plays many important roles in fertility and maintenance of pregnancy. PAEP changes its biochemical interaction based on its glycosylation state. PAEP is a prognostic marker for in vitro fertilization and for premature membrane rupture. The antibodies in this kit were raised against a recombinant full-length mature protein. This protein does not have a homolog in mouse or rat and is not expected to cross-react with samples from these species.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Pre-coated microplate (12 x 8 well strips)
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human PAEP (Glycodelin) Capture Antibody 1 x 600ml 10X Human PAEP (Glycodelin) Detector Antibody 1 x 600ml 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human PAEP (Glycodelin) Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
This protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy. -
序列相似性
Belongs to the calycin superfamily. Lipocalin family. -
翻译后修饰
At least two differentially glycosylated forms are found. Glycodelin-A (GdA) (amniotic fluid) with contraceptive and immunosuppressive activities and glycodelin-S (Gds) (seminal plasma) whose role is not yet known. The gender-specific glycosylation may serve to regulate key process involved in human reproduction. -
细胞定位
Secreted. - Information by UniProt
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别名
- Alpha uterine protein
- gD
- GdA
see all -
数据库链接
- Entrez Gene: 5047 Human
- Omim: 173310 Human
- SwissProt: P09466 Human
- Unigene: 532325 Human
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab275904于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Human PAEP standard curve in Sample Diluent NS. -
Interpolated concentrations of native PAEP in Human serum samples. -
Sandwich ELISA - Human PAEP ELISA Kit (Glycodelin) (ab275904)To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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