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AB275904

人PAEP ELISA试剂盒(Glycodelin)

Human PAEP ELISA Kit (Glycodelin)

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Human PAEP ELISA Kit (Glycodelin) is a single-wash 90-min Simplestep used to quantify Human PAEP (Glycodelin) with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

Glycodelin, GD, Placental protein 14, Pregnancy-associated endometrial alpha-2 globulin, Progestagen-associated endometrial protein, Progesterone-associated endometrial protein, Zona-binding inhibitory factor-1, PP14, PAEG, PEG, ZIF-1, PAEP

2 Images
Sandwich ELISA - Human PAEP ELISA Kit (Glycodelin) (AB275904)
  • sELISA

Supplier Data

Sandwich ELISA - Human PAEP ELISA Kit (Glycodelin) (AB275904)

Example of Human PAEP standard curve in Sample Diluent NS

Sandwich ELISA - Human PAEP ELISA Kit (Glycodelin) (AB275904)
  • sELISA

Supplier Data

Sandwich ELISA - Human PAEP ELISA Kit (Glycodelin) (AB275904)

Interpolated concentrations of native PAEP in Human serum samples

关键信息

检测方法

Colorimetric

样品类型

Serum

反应种属

Human

检测类型

Sandwich

灵敏度

= 3 pg/mL

范围

7.81 - 500 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

产品详情

Human PAEP ELISA kit (ab275904) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PAEP protein in human serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PAEP with 3 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

PAEP, progestogen-associated endometrial protein also known as glycodelin, is a glycoprotein which plays many important roles in fertility and maintenance of pregnancy. PAEP changes its biochemical interaction based on its glycosylation state. PAEP is a prognostic marker for in vitro fertilization and for premature membrane rupture. The antibodies in this kit were raised against a recombinant full-length mature protein. This protein does not have a homolog in mouse or rat and is not expected to cross-react with samples from these species.

精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.98" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]

回收率

[ { "sample": "Serum", "range": "89 - 116 %", "average": "= 107" } ]

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab275904-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB275904-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB275904-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB275904-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB275904-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB275904-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB275904-CMP09", "productcode":"" }, { "size":"1 x 600 mL", "name":"10X Human PAEP (Glycodelin) Capture Antibody", "number":"AB275904-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB275904-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PAEP (Glycodelin) Lyophilized Recombinant Protein", "number":"AB275904-CMP08", "productcode":"" }, { "size":"1 x 600 mL", "name":"10X Human PAEP (Glycodelin) Detector Antibody", "number":"AB275904-CMP03", "productcode":"" } ] } } }

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C

产品实验方案

靶点信息

Glycoprotein that regulates critical steps during fertilization and also has immunomonomodulatory effects. Four glycoforms, namely glycodelin-S, -A, -F and -C have been identified in reproductive tissues that differ in glycosylation and biological activity. Glycodelin-A has contraceptive and immunosuppressive activities (PubMed : 7531163, PubMed : 9918684). Glycodelin-C stimulates binding of spermatozoa to the zona pellucida (PubMed : 17192260). Glycodelin-F inhibits spermatozoa-zona pellucida binding and significantly suppresses progesterone-induced acrosome reaction of spermatozoa (PubMed : 12672671). Glycodelin-S in seminal plasma maintains the uncapacitated state of human spermatozoa (PubMed : 15883155).
See full target information Glycodelin
websiteProtocolBooklet
en

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