JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB242248

人Oxytocin-neurophysin 1 Prepropeptide ELISA试剂盒

Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Oxytocin-neurophysin 1 Prepropeptide with a sensitivity of 10 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

OT, OXT, Oxytocin-neurophysin 1, OT-NPI

3 Images
ELISA - Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit (AB242248)
  • ELISA

Supplier Data

ELISA - Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit (AB242248)
Sandwich ELISA - Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit (AB242248)
  • sELISA

Unknown

Sandwich ELISA - Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit (AB242248)

Interpolated concentrations of native Oxytocin-neurophysin 1 in human serum and plasma samples.

The concentrations of Oxytocin-neurophysin 1 were measured in duplicates, interpolated from the Oxytocin-neurophysin 1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 50%, plasma (EDTA) 25%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Oxytocin-neurophysin 1 concentration was determined to be 23,301 pg/mL in serum, 13,420 pg/mL in plasma (citrate), 21,214 pg/mL in plasma (EDTA), and 20843 pg/mL in plasma (Heparin).

Sandwich ELISA - Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit (AB242248)
  • sELISA

Unknown

Sandwich ELISA - Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit (AB242248)

Example of human Oxytocin-neurophysin 1 standard curve in Sample Diluent NS.

The Oxytocin-neurophysin 1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich

灵敏度

= 10 pg/mL

范围

125 - 8000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit ab242248 is a rapid single-wash 90-min Sandwich ELISA to measure Human Oxytocin-neurophysin 1 Prepropeptide in citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 10 pg/mL.

How the assay works

Human Oxytocin-neurophysin 1 Prepropeptide SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Oxytocin-neurophysin 1 Prepropeptide ELISA Kit ab242248 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
style
left-column

精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
style
left-column

回收率

[ { "sample": "Serum", "range": "84 - 100 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "95 - 111 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "94 - 113 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "83 - 99 %", "average": "= 92" } ]
style
left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab242248-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB242248-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Oxytocin-neurophysin 1 Prepropeptide Capture Antibody", "number":"AB242248-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB242248-CMP14", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB242248-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Oxytocin-neurophysin 1 Prepropeptide Lyophilized Recombinant Protein", "number":"AB242248-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB242248-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB242248-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB242248-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Oxytocin-neurophysin 1 Prepropeptide Detector Antibody", "number":"AB242248-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB242248-CMP09", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Oxytocin-neurophysin 1 also known as prepro-oxytocin-neurophysin I is a precursor protein with a molecular weight of approximately 20 kDa. This protein is expressed primarily in the hypothalamus and is involved in the synthesis and storage of the hormone oxytocin a peptide essential for various physiological processes. During synthesis the protein undergoes cleavage to separate oxytocin and neurophysin 1 a carrier protein that aids in the transport and stabilization of oxytocin in neurosecretory granules.
Biological function summary

Oxytocin-neurophysin 1 plays an important role in the regulation of social behaviors reproductive functions and fluid homeostasis. This precursor is not part of a larger protein complex but neurophysin 1 serves as an essential carrier that binds and transports oxytocin to its target sites in the body. Oxytocin itself functions as a neuromodulator and hormone exerting effects on the uterine muscles and mammary glands promoting childbirth and lactation.

Pathways

The biosynthesis and release of oxytocin-neurophysin 1 are closely associated with the hypothalamic neurosecretory pathways. These pathways involve the neurohypophysis where oxytocin is released into the bloodstream. Additionally oxytocin interacts with the G-protein-coupled receptor OXTR to elicit its physiological effects. V1a and V2 receptors alongside oxytocin play roles in various signaling pathways that affect metabolic and cardiovascular functions.

Dysregulation of oxytocin-neurophysin 1 has been linked to the syndrome of inappropriate antidiuretic hormone secretion (SIADH) and autism spectrum disorders. In these conditions altered levels of oxytocin affect fluid balance and social behavior. Additionally changes in oxytocin levels often in tandem with altered vasopressin regulation associate with the development and exacerbation of these disorders.
style
left-column

产品实验方案

style
left-column

靶点信息

Neurophysin 1 specifically binds oxytocin.. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Acts by binding to oxytocin receptor (OXTR) (PubMed : 18174156).
See full target information OXT
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

搭配使用产品

Assay Kits

AB212166

Human BDNF ELISA Kit

SimpleStep

ELISA - Human BDNF ELISA Kit (AB212166)

  • 4
  • 2
Assay Kits

AB289701

Human Vasopressin-neurophysin 2-copeptin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Vasopressin-neurophysin 2-copeptin ELISA Kit (AB289701)

  • 0
  • 0
Assay Kits

AB108665

Cortisol ELISA Kit

Competitive ELISA - Cortisol ELISA Kit (AB108665)

  • 5
  • 4
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com