AB270202

人Osteocalcin ELISA试剂盒

Name: 人Osteocalcin ELISA试剂盒 (ab270202)| Abcam中文官网
Brand: Abcam Limited
SKU: AB270202
Price: 8117 CNY
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Human Osteocalcin ELISA Kit

Recombinant
SimpleStep
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(1 Review)

(13 Publications)

Human Osteocalcin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Osteocalcin with a sensitivity of 13.99 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

查看别名

Osteocalcin, Bone Gla protein, Gamma-carboxyglutamic acid-containing protein, BGP, BGLAP

6 Images

ELISA - Human Osteocalcin ELISA Kit (AB270202)

ELISA

Supplier Data

ELISA - Human Osteocalcin ELISA Kit (AB270202)

sELISA

Supplier Data

Sandwich ELISA - Human Osteocalcin ELISA Kit (AB270202)

Measurement of CHIT1 activity.

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteocalcin concentration was determined to be 18.95 ng/mL with a range of 1.30 – 38.94 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Osteocalcin ELISA Kit (AB270202)

Example of human Osteocalcin standard curve in Sample Diluent NS.

The Osteocalcin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Osteocalcin ELISA Kit (AB270202)

Human osteocalcin standard curve comparison.

Standard Curve comparison between the original Human Ostecalcin SimpleStep ELISA kit (ab270202) and current Human Ostecalcin SimpleStep ELISA kit (ab195214).

sELISA

Supplier Data

Sandwich ELISA - Human Osteocalcin ELISA Kit (AB270202)

Interpolated concentrations of native Osteocalcin in human serum and plasma samples.

The concentrations of Osteocalcin were measured in duplicates, interpolated from the Osteocalcin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 7.5%, plasma (citrate) 10%, plasma (heparin) 7.5%, and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteocalcin concentration was determined to be 34.49 ng/mL in serum, 24.94 ng/mL in plasma (citrate), 41.51 ng/ml in plasma (heparin), and 32.69 ng/ml in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human Osteocalcin ELISA Kit (AB270202)

Human Osteocalcin competitor curve comparison.

Standard Curve comparison between human osteocalcin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

关键信息

检测方法

Colorimetric

样品类型

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 13.99 pg/mL

范围

52.08 - 3333 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human Osteocalcin ELISA Kit ab270202 is a rapid single-wash 90-min Sandwich ELISA to measure Human Osteocalcin in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 13.99 pg/mL.

How the assay works

Human Osteocalcin SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Osteocalcin ELISA Kit ab270202 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Osteocalcin antibody [EPR22338-241] - BSA and Azide free (Capture) ab272276
- Anti-Osteocalcin antibody [EPR22338-92] - BSA and Azide free (Detector) ab272276

Osteocalcin is a small secreted protein 49 amino acids produced by osteoblasts. Osteocalcin acts as a hormone impacting bone mineral absorption, glucose metabolism and insulin secretion. During bone reabsorption osteoclasts break down the bone matrix releasing bound Osteocalcin into 5 different fragments; N-terminal, Mid, C-terminal, N-terminal-Mid, and Mid-C-terminal into the serum. Circulating levels of intact Osteocalcin and Osteocalcin fragments have been found to vary significantly between healthy individuals and diseased stats such as Paget's disease, osteoporosis, fracture, and renal failure. The standard protein in this product is mature Osteocalcin.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]

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回收率

[ { "sample": "Serum", "range": "80 - 103 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "90 - 96 %", "average": "= 93" }, { "sample": "Cell culture media", "range": "91 - 115 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "81 - 99 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "84 - 97 %", "average": "= 90" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab270202-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB270202-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB270202-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB270202-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB270202-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB270202-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Osteocalcin Lyophilized Recombinant Protein", "number":"AB270202-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB270202-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Osteocalcin Capture Antibody", "number":"AB270202-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Osteocalcin Detector Antibody", "number":"AB270202-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB270202-CMP04", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Osteocalcin also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP) is a small non-collagenous protein with a molecular weight of approximately 5.8 kDa. This protein is mainly expressed in osteoblasts which are bone-forming cells. It is an important component of the bone matrix and is involved in bone mineralization. Osteocalcin binds to calcium and incorporates into the hydroxyapatite crystals in the bone helping stabilize the bone structure. Researchers often study this protein as a marker for bone formation and turnover.

Biological function summary

Osteocalcin plays a significant role in regulating glucose metabolism and fat deposition. It acts as a hormone from the bone and influences energy metabolism and insulin sensitivity. Osteocalcin undergoes carboxylation a post-translational modification necessary for its function in bone. Beyond its structural role demineralized osteocalcin has been found to impact pancreatic β-cell function adipose tissue and male fertility although the pathways in these processes are not entirely understood yet.

Pathways

Osteocalcin is associated with the energy metabolism and bone remodeling pathways. It interacts with the insulin signaling pathway influencing insulin secretion by pancreatic β-cells and boosting insulin sensitivity. This interaction involves proteins like insulin receptor substrate 1 (IRS1) and glucose transporter type 4 (GLUT4) which play roles in glucose uptake. Osteocalcin also connects to bone remodeling pathways by influencing osteoclast differentiation and activity through osteoprotegrin and receptor activator of nuclear factor kappa-B ligand (RANKL).

Dysregulation of osteocalcin levels associates with metabolic syndrome and osteoporosis. It is linked with insulin resistance affecting glucose homeostasis and potentially contributing to diabetes development. Osteocalcin's involvement in bone density maintenance also makes it relevant in osteoporosis where bone formation balance is disrupted. The receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegrin which osteocalcin interacts with are other proteins connected to the pathogenesis of these conditions. Understanding osteocalcin's function helps in exploring therapeutic targets for these diseases.

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产品实验方案

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靶点信息

Bone protein that constitutes 1-2% of the total bone protein, and which acts as a negative regulator of bone formation (PubMed : 3019668, PubMed : 6967872). Functions to limit bone formation without impairing bone resorption or mineralization (By similarity). It binds strongly to apatite and calcium (PubMed : 6967872).. The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development. Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure. Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes : acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner. Also acts as a regulator of brain development : osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development.

See full target information BGLAP

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:294 PubMed40483499

2025

Metabolic markers detect early ostedifferentiation of mesenchymal stem cells from multiple donors.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela S C Bispo,Inês C R Graça,Catarina S H Jesus,João E Rodrigues,Marlene C Correia,Sabrina Atella,Iola F Duarte,Brian J Goodfellow,Mariana B Oliveira,João F Mano,Ana M Gil

Cells 14: PubMed40358181

2025

Effect of Recombinant Human Amelogenin on the Osteogenic Differentiation Potential of SHED.

Applications

Unspecified application

Species

Unspecified reactive species

Akira Hirabae,Ryo Kunimatsu,Yuki Yoshimi,Kodai Rikitake,Shintaro Ogashira,Ayaka Nakatani,Shuzo Sakata,Kotaro Tanimoto

Cureus 16:e59704 PubMed38841020

2024

Exercise-Induced Alterations in Irisin and Osteocalcin Levels: A Comparative Analysis Across Different Training Modalities.

Applications

Unspecified application

Species

Unspecified reactive species

P Adilakshmi,V Suganthi,K Balu Mahendran,K Satyanarayana Rao,B Savithri

JCI insight 9: PubMed38780544

2024

Methionine as a regulator of bone remodeling with fasting.

Applications

Unspecified application

Species

Unspecified reactive species

Tânia Amorim,Naveen Gv Kumar,Natalie L David,William Dion,Trishya Pagadala,Nandini K Doshi,Bokai Zhu,Andrey Parkhitko,Matthew L Steinhauser,Pouneh K Fazeli

PloS one 17:e0276328 PubMed36548302

2022

Xianling Gubao attenuates high glucose-induced bone metabolism disorder in MG63 osteoblast-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xinlong Chen,Yan Li,Zhongwen Zhang,Liping Chen,Yaqian Liu,Shuhong Huang,Xiaoqian Zhang

Computational and mathematical methods in medicine 2022:9089084 PubMed35991151

2022

Effect of nHA-Coated Femoral Stem Prosthesis Combined with Platelet-Rich Plasma in Hemi Hip Replacement of Femoral Neck Fracture in the Elderly.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Yang,Dabin Wu,Tongsheng Liu,Guozheng Fang

Materials (Basel, Switzerland) 15: PubMed35955174

2022

Evaluation of the Biocompatibility and Osteogenic Properties of Metal Oxide Coatings Applied by Magnetron Sputtering as Potential Biofunctional Surface Modifications for Orthopedic Implants.

Applications

Unspecified application

Species

Unspecified reactive species

Mariana Fernández-Lizárraga,Julieta García-López,Sandra E Rodil,Rosa María Ribas-Aparicio,Phaedra Silva-Bermudez

Pharmaceutics 14: PubMed35745803

2022

Human Osteochondral Explants as an Ex Vivo Model of Osteoarthritis for the Assessment of a Novel Class of Orthobiologics.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Giannasi,Laura Mangiavini,Stefania Niada,Andrea Colombo,Elena Della Morte,Valeria Vismara,Andrea Ambrosanio,Paolo Savadori,Sara Casati,Giuseppe M Peretti,Anna Teresa Brini

Journal of musculoskeletal & neuronal interactions 22:242-250 PubMed35642703

2022

Foxf1 gene increases the risk of osteoporosis in rats by inhibiting osteoblast formation and promoting osteoclast differentiation through the upregulation of NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Ouyang,Shimin Li,Yunzhi Ding,Feng Xin,Meng Liu

Tissue engineering. Part C, Methods 28:335-350 PubMed35323028

2022

Cholecalciferol as Bioactive Plasticizer of High Molecular Weight Poly(D,L-Lactic Acid) Scaffolds for Bone Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Roberto Calore,Darya Hadavi,Maarten Honing,Ane Albillos-Sanchez,Carlos Mota,Katrien Bernaerts,Jules Harings,Lorenzo Moroni

View all publications

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websiteProtocolBooklet

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人Osteocalcin ELISA试剂盒

Human Osteocalcin ELISA Kit

ELISA - Human Osteocalcin ELISA Kit (AB270202)

Sandwich ELISA - Human Osteocalcin ELISA Kit (AB270202)

Sandwich ELISA - Human Osteocalcin ELISA Kit (AB270202)

Sandwich ELISA - Human Osteocalcin ELISA Kit (AB270202)

Sandwich ELISA - Human Osteocalcin ELISA Kit (AB270202)

Sandwich ELISA - Human Osteocalcin ELISA Kit (AB270202)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台