Human OLFM4 ELISA Kit is a single-wash 90-min SimpleStep ELISA used to quantify Human OLFM4 with a sensitivity of 315.7 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab326735
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GW112, UNQ362/PRO698, OLFM4, Olfactomedin-4, OLM4, Antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein, hOLfD, hGC-1
- ELISA
Supplier Data
ELISA - Human OLFM4 ELISA Kit (AB267805)
- sELISA
Supplier Data
Sandwich ELISA - Human OLFM4 ELISA Kit (AB267805)
Interpolated concentrations of native OLFM4 in human serum and plasma samples.
The concentrations of OLFM4 were measured in duplicates, interpolated from the OLFM4 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100% and plasma (citrate) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OLFM4 concentration was determined to be 4,710 pg/mL in serum and 4,029 pg/mL in plasma (citrate).
- sELISA
Supplier Data
Sandwich ELISA - Human OLFM4 ELISA Kit (AB267805)
Interpolated concentrations of native OLFM4 in human colon tissue extract based on a 400 μg/mL extract load.
The concentrations of OLFM4 were measured in duplicate and interpolated from the OLFM4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OLFM4 concentration was determined to be 48,973 pg/mL in colon tissue extract.
- sELISA
Supplier Data
Sandwich ELISA - Human OLFM4 ELISA Kit (AB267805)
Example of human OLFM4 standard curve in Sample Diluent NS.
The OLFM4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
搭配使用产品
Assay Kits
AB326735
Human OLFM4 ELISA Kit, Chemiluminescent
SimpleStep Ignite™ ELISA|Recombinant
- 0
- 0
反应性数据
产品详情
Human OLFM4 ELISA Kit ab267805 is a rapid single-wash 90-min Sandwich ELISA to measure Human OLFM4 in cell culture media, citrate plasma, serum, tissue extracts. This SimpleStep sensitivity is 315.7 pg/mL.
How the assay works
Human OLFM4 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human OLFM4 ELISA Kit ab267805 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-OLFM4 antibody [EPR22335-74] - BSA and Azide free (Capture) ab269391
- Anti-OLFM4 antibody [EPR22335-163] - BSA and Azide free (Detector) ab269391
Olfactomedin-4 (OLFM4), formerly known as GW112 or hGC-1, is a member of the olfactomedin gene family and predominantly expressed in bone marrow, small intestine, colon, and prostrate. Studies have revealed OLFM4 to play important roles in several cellular functions such as neurogenesis, cell adhesion, cell-cycle regulation, and tumorigenesis. Moreover, studies have suggested OLFM4 to serve as a robust biomarker in studying stem cells and cancer in small intestines, stomach, and colon.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
OLFM4 functions in inhibiting bacterial growth by interfering with bacterial adhesion and survival. It is not part of a larger protein complex but interacts with cell surface proteins to exert its effects. OLFM4 acts in the regulation of cell differentiation and growth playing a significant part in maintaining the stem cell milieu in the intestine.
Pathways
OLFM4 participates in cellular defense mechanisms primarily against microbial pathogens by involving in the activation of immune response pathways. It is associated with pathways such as the NF-kB signaling and Janus kinase (JAK) pathways. Through these pathways OLFM4 might interact with cytokines and other inflammatory mediators or modulators.
靶点信息
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Recent publications for all applications. Explore the full list and refine your search
Journal of innate immunity 15:351-364 PubMed36450268
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Journal of inflammation research 14:7035-7051 PubMed34955649
2021
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Canadian journal of gastroenterology & hepatology 2021:5601678 PubMed34912753
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