AB277467

人NPTXR ELISA试剂盒

Name: 人NPTXR ELISA试剂盒 (ab277467)| Abcam中文官网
Brand: Abcam Limited
SKU: AB277467
Price: 6541 CNY
Availability: InStock

Human NPTXR ELISA Kit

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Human NPTXR ELISA Kit is a Sandwich ELISA for the measurement of Human NPTXR in Human in Biofluids, Cell Culture Media samples.

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Neuronal pentraxin receptor, NPTXR

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ELISA - Human NPTXR ELISA Kit (AB277467)

ELISA

Supplier Data

ELISA - Human NPTXR ELISA Kit (AB277467)

These standard curves are for demonstration only. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich

灵敏度

= 0.4 ng/mL

范围

0.41 - 100 ng/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human NPTXR ELISA Kit (ab277467) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NPTXR in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human NPTXR coated on a 96-well plate. Standards and samples are pipetted into the wells and Human NPTXR present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human NPTXR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human NPTXR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

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回收率

[ { "sample": "Serum", "range": "70 - 78 %", "average": "= 73.88" }, { "sample": "Plasma", "range": "68 - 90 %", "average": "= 75.32" }, { "sample": "Cell culture media", "range": "68 - 94 %", "average": "= 78.46" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277467-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277467-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277467-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human NPTXR coated Microplate", "number":"AB277467-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human NPTXR Antibody", "number":"AB277467-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB277467-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277467-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB277467-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human NPTXR standard protein (Lyophilized)", "number":"AB277467-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277467-CMP08", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NPTXR also known as Neuronal Pentraxin Receptor is a protein involved in the synaptic organization. It has a molecular mass of approximately 65 kDa. NPTXR expresses mainly in the central nervous system including the brain and spinal cord. As a synaptic protein it plays a role in modulating neuron-neuron interactions and signaling pathways critical for the central nervous system's proper function.

Biological function summary

Neuronal pentraxin receptor contributes to synaptic plasticity and maintenance. It forms a complex with other pentraxin proteins like NPTX1 and NPTX2 facilitating the clustering of AMPA receptors at synapses. This interaction enhances excitatory synaptic transmission which is important for processes like learning and memory. NPTXR therefore plays a significant role in maintaining synaptic strength and integrity.

Pathways

The neuronal pentraxin receptor influences the glutamatergic signaling pathway. This pathway involves neurotransmitter glutamate and its receptors which include AMPA-type glutamate receptors at postsynaptic sites. NPTXR's interaction with AMPA receptors via NPTX1 and NPTX2 promotes receptor clustering critical for NMDA receptor phosphorylation. As a result this protein contributes to synaptic plasticity influencing learning and memory pathways in neurons.

NPTXR has implications in neurodegenerative diseases and schizophrenia. Altered synaptic plasticity and receptor clustering can lead to cognitive deficits frequently observed in Alzheimer's disease. Additionally studies suggest a potential link between NPTXR and schizophrenia where dysregulated synaptic organization impacts neurotransmission. NPTXR's association with AMPA receptors through other pentraxins implicates it in these disorders highlighting its significance as a therapeutic target.

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产品实验方案

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靶点信息

May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.

See full target information NPTXR

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websiteProtocolBooklet

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人NPTXR ELISA试剂盒

Human NPTXR ELISA Kit

ELISA - Human NPTXR ELISA Kit (AB277467)