JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB155436

人NGF R ELISA试剂盒(CD271)

Human NGF R ELISA Kit (CD271)

Be the first to review this product! Submit a review

|

(1 Publication)

Human NGF R ELISA Kit (CD271) is a Sandwich ELISA for the measurement of Human NGF R (CD271) in Human in Biofluids, Cell Culture Media samples.

查看别名

CD271, TNFRSF16, NGFR, Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR, p75 ICD, NGF receptor

1 Images
Sandwich ELISA - Human NGF R ELISA Kit (CD271) (AB155436)
  • sELISA

Unknown

Sandwich ELISA - Human NGF R ELISA Kit (CD271) (AB155436)

NGF R (CD271) Human ELISA kit.

Representative standard curve using ab155436

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

< 80 pg/mL

范围

0.082 - 20 ng/mL

检测平台

Microplate

style
left-column

搭配使用产品

Assay Kits

AB193760

Human beta Nerve Growth Factor ELISA Kit

SimpleStep

ELISA - Human beta Nerve Growth Factor ELISA Kit (AB193760)

  • 5
  • 1
Primary Antibodies

AB195180

Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-p75 NGF Receptor antibody [EP1039Y] (AB195180)

  • 5
  • 1
Assay Kits

AB100525

Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor)

Sandwich ELISA - Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor) (AB100525)

  • 4
  • 1
Proteins & Peptides

AB307634

Recombinant Human p75 NGF Receptor Protein (Fc Chimera) (Active)

Premium Bioactive

Functional Studies - Recombinant Human p75 NGF Receptor Protein (Fc Chimera) (Active) (AB307634)

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Abcam's NGF R (CD271) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human NGF R (CD271) in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human NGF R coated on a 96-well plate. Standards and samples are pipetted into the wells and NGF R present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human NGF R antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NGF R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

The standard is affinity purified.

style
left-column

精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

回收率

[ { "sample": "Cell culture supernatant", "range": "79 - 95 %", "average": "= 86.88" }, { "sample": "Serum", "range": "75 - 93 %", "average": "= 82.75" }, { "sample": "Plasma", "range": "76 - 93 %", "average": "= 84.17" } ]
style
left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155436-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155436-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155436-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB155436-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"NGF R Microplate (12 x 8 wells)", "number":"AB155436-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155436-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155436-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human NGF R Standard (lyophilized)", "number":"AB155436-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human NGF R", "number":"AB155436-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB155436-CMP03", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
style
left-column
style
left-column

产品实验方案

style
left-column

靶点信息

Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF (PubMed : 24908487). Plays an important role in differentiation and survival of specific neuronal populations during development (By similarity). Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA (PubMed : 26646181). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Necessary for the circadian oscillation of the clock genes BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver (PubMed : 23785138). Together with BFAR negatively regulates NF-kappa-B and JNK-related signaling pathways (PubMed : 22566094).
See full target information NGFR
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Translational psychiatry 7:e1079 PubMed28375203

2017

The serum protein levels of the tPA-BDNF pathway are implicated in depression and antidepressant treatment.

Applications

ELISA

Species

Unspecified reactive species

H Jiang,S Chen,C Li,N Lu,Y Yue,Y Yin,Y Zhang,X Zhi,D Zhang,Y Yuan
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com