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AB325362

Human Neutrophil Elastase ELISA Kit, Chemiluminescent

Human Neutrophil Elastase ELISA Kit, Chemiluminescent

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Human Neutrophil Elastase ELISA Kit, Chemiluminescent is a 55-min single-wash Simplestep IgniteTM ELISA for the rapid quantification of Human Neutrophil Elastase with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :4.6 - 10000 pg/mL with a sensitivity of 2 pg/mL
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

ELA2, ELANE, Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, Medullasin, PMN elastase, HLE

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Sandwich ELISA - Human Neutrophil Elastase ELISA Kit, Chemiluminescent (AB325362)
  • sELISA

Lab

Sandwich ELISA - Human Neutrophil Elastase ELISA Kit, Chemiluminescent (AB325362)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

关键信息

检测方法

Luminescent

样品类型

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Cell Extract, Cerebral Spinal Fluid

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 2 pg/mL

范围

4.6 - 10000 pg/mL

检测时间

55m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human Neutrophil Elastase ELISA Kit, Chemiluminescent (ab325362) is a rapid single-wash sandwich 55-min ELISA to measure Human Neutrophil Elastase in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Media, Cerebrospinal Fluid, Cell Extract with a sensitivity of 2 pg/ml.

How the assay works

Human Neutrophil Elastase ELISA Kit, Chemiluminescent (ab325362) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time.  By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Please refer to our protocol booklet for more details.

Human Neutrophil Elastase (ab325362) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Rabbit monoclonal [EPR22896-31] to Neutrophil elastase - BSA and Azide free (Capture) ab272279

- Rabbit monoclonal [EPR22896-78] to Neutrophil elastase - BSA and Azide free (Detector) ab272280

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
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回收率

[ { "sample": "Serum", "range": "105 - 120 %", "average": "= 112" }, { "sample": "Citrate plasma", "range": "100 - 105 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "103 - 118 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "105 - 113 %", "average": "= 108" }, { "sample": "Cell culture media", "range": "103 - 109 %", "average": "= 106" }, { "sample": "Cerebral Spinal Fluid", "range": "91 - 106 %", "average": "= 97" }, { "sample": "Cell Lysate", "range": "115 - 117 %", "average": "= 116" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325362-1x96Tests", "assetComponentDetails": [ { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325362-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325362-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Neutrophil Elastase Capture Antibody", "number":"AB325362-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BS", "number":"AB325362-CMP05", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325362-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB325362-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325362-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325362-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Neutrophil Elastase Detector Antibody", "number":"AB325362-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325362-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB325362-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Neutrophil Elastase Lyophilized Recombinant Protein", "number":"AB325362-CMP03", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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产品实验方案

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靶点信息

Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed : 15140022). Promotes cleavage of GSDMB, thereby inhibiting pyroptosis (PubMed : 36899106). Promotes blood coagulation (PubMed : 20676107). Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG) (PubMed : 25211214, PubMed : 9111081). Cleaves and thus inactivates tissue factor pathway inhibitor (TFPI) (PubMed : 20676107, PubMed : 25211214). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed : 10947984).
See full target information ELANE
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websiteProtocolBooklet
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Abcam Product Promise

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