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AB283539

人Neuroserpin ELISA试剂盒

Human Neuroserpin ELISA Kit

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(1 Publication)

Human Neuroserpin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Neuroserpin with a sensitivity of 25 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

PI12, SERPINI1, Neuroserpin, Peptidase inhibitor 12, Serpin I1, PI-12

3 Images
Sandwich ELISA - Human Neuroserpin ELISA Kit (AB283539)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neuroserpin ELISA Kit (AB283539)

Interpolated concentrations of native Neuroserpin in human serum and plasma samples.

The concentrations of Neuroserpin were measured in duplicates, interpolated from the Neuroserpin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma-citrate 50%, plasma-heparin 50% and plasma-edta 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuroserpin concentration was determined to be 5464 pg/mL in serum, 5,291 pg/mL in plasma-citrate, 5,070 pg/mL in plasma-edta, and 5,265 pg/mL in plasma-heparin.

Sandwich ELISA - Human Neuroserpin ELISA Kit (AB283539)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neuroserpin ELISA Kit (AB283539)

Serum from ten individual healthy human gender donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neuroserpin concentration was determined to be 3.74 ng/mL with a range of 1.62 – 6.02 ng/mL.

Sandwich ELISA - Human Neuroserpin ELISA Kit (AB283539)
  • sELISA

Supplier Data

Sandwich ELISA - Human Neuroserpin ELISA Kit (AB283539)

Example of human Neuroserpin standard curve in 1X Cell Extraction Buffer PTR.

The Neuroserpin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich

灵敏度

= 25 pg/mL

范围

109.38 - 7000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

产品详情

Human Neuroserpin ELISA kit (ab283539) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Neuroserpin protein in human serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Neuroserpin with 25 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Neuroserpin is a serine protease which plays a key role in neural growth regulation, protection from ischemic damage, learning, and behavior. It plays a key role in extra cellular matrix homeostasis through its regulatory role over the enzyme tissue-type plasminogen activator. Dysregulation of this protein is a hallmark of familial encephalopathy. The immunogen from which the antibodies in this kit were raised represent the full-length mature protein. This protein shares only 88% and 89% sequence identity with mouse and rat, respectively, and is not expected to be highly cross-reactive.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]

回收率

[ { "sample": "Serum", "range": "82 - 95 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "83 - 102 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "91 - 97 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "91 - 97 %", "average": "= 94" } ]

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283539-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB283539-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB283539-CMP13", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Neuroserpin Capture Antibody", "number":"AB283539-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB283539-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB283539-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Neuroserpin Lyophilized Recombinant Protein", "number":"AB283539-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB283539-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB283539-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB283539-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Neuroserpin Detector Antibody", "number":"AB283539-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB283539-CMP07", "productcode":"" } ] } } }

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Neuroserpin also known by its alternate name Serpin I1 is a member of the serine protease inhibitor family. It weighs about 44 kDa. Neuroserpin expresses mainly in the nervous system particularly in the brain and spinal cord where it regulates proteolytic activity. It localizes to the endoplasmic reticulum and is secreted to the extracellular space influencing neural processes.
Biological function summary

Neuroserpin plays an important role in nervous system development and maintenance. It inhibits tissue plasminogen activator (tPA) a protease involved in breaking down blood clots and controls extracellular matrix modifications. Neuroserpin does not form part of a complex but interacts with other key proteins for its function. Its regulation of tPA influences synaptic plasticity and protection of neurons from excessive proteolysis which is essential in maintaining proper brain function.

Pathways

Neuroserpin's activity is significant in the plasminogen activation system and neurotrophic pathways. It influences the balance of proteolytic activities that affect cell migration and synaptic remodeling. Related proteins in these pathways include proteases like tPA and neurotrophins like brain-derived neurotrophic factor (BDNF) which support neuronal survival.

Mutations in neuroserpin are linked to familial encephalopathy with neuroserpin inclusion bodies (FENIB) and Alzheimer's disease. FENIB is caused by neuroserpin polymerization and accumulation leading to neurodegeneration. In Alzheimer's disease neuroserpin interaction with amyloid precursor protein pathways contributes to plaque formation and neuronal dysfunction. Neuroserpin's connection with defective tPA regulation underlies its association with these neurodegenerative conditions.

产品实验方案

靶点信息

Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin (PubMed : 11880376, PubMed : 19265707, PubMed : 19285087, PubMed : 26329378, PubMed : 9442076). May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator (Probable).
See full target information SERPINI1

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

EJHaem 4:324-338 PubMed37206290

2023

SARS-CoV-2 infection results in upregulation of Plasminogen Activator Inhibitor-1 and Neuroserpin in the lungs, and an increase in fibrinolysis inhibitors associated with disease severity.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin H Toomer,Gloria F Gerber,Yifan Zhang,Laetitia Daou,Michael Tushek,Jody E Hooper,Ivo M B Francischetti
View all publications
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