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AB309453

人NELL-1 ELISA

Human NELL-1 ELISA

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Human NELL-1 ELISA is a Sandwich ELISA for the measurement of Human NELL-1 ELISA in Human in Biofluids, Cell Culture Media samples.

查看别名

NRP1, NELL1, Protein kinase C-binding protein NELL1, NEL-like protein 1, Nel-related protein 1

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Sandwich ELISA - Human NELL-1 ELISA (AB309453)
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Supplier Data

Sandwich ELISA - Human NELL-1 ELISA (AB309453)

Example of standard curve using ab309453. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

关键信息

检测方法

Colorimetric

样品类型

Serum, Plasma, Cell culture supernatant

反应种属

Human

检测类型

Sandwich

灵敏度

= 10 pg/mL

范围

10 - 200 ng/mL

检测平台

Pre-coated microplate (12 x 8 well strips)

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产品详情

Human NELL-1 ELISA is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human NELL-1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human NELL-1 coated on a 96-well plate.
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回收率

[ { "sample": "Serum", "range": "97 - 137 %", "average": "= 114.3" }, { "sample": "Plasma", "range": "85 - 138 %", "average": "= 114.5" }, { "sample": "Cell culture supernatant", "range": "91 - 109 %", "average": "= 100.2" }, { "sample": "Cell culture media", "range": "91 - 109 %", "average": "= 100.2" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309453-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB309453-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB309453-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309453-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human NELL1 Detection Antibody", "number":"AB309453-CMP14", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human NELL1 Protein Standard", "number":"AB309453-CMP16", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human NELL1 Antibody-coated ELISA Plate", "number":"AB309453-CMP15", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB309453-CMP13", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309453-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309453-CMP05", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NELL1 also known as Neural EGFL Like 1 is a protein primarily involved in cell signaling processes. Its molecular weight is approximately 87 kDa. NELL1 expresses in tissues such as bone cartilage blood vessels and the nervous system. It functions as a secreted glycoprotein and may play a role in mediating cellular communication.
Biological function summary

This protein influences cell growth differentiation and apoptosis. NELL1 is not known to be part of a multiprotein complex but it interacts with various factors that regulate tissue development. It has significant functions in osteogenesis and angiogenesis impacting bone formation and blood vessel formation. The presence of NELL1 is essential for normal skeletal development and repair.

Pathways

Multiple molecular interactions involve NELL1 in critical signaling pathways. NELL1 acts in the BMP signaling pathway an important player in bone and cartilage development. It also interacts with TGF-beta signaling pathways which regulate cell proliferation and differentiation. These interactions link NELL1 to proteins like BMP2 and TGF-beta receptors highlighting its role in cellular signaling dynamics.

NELL1 has associations with conditions like craniosynostosis and osteoporosis. Altered expression of NELL1 contributes to the premature fusion of skull bones in craniosynostosis. In osteoporosis NELL1 influences bone density and structure abnormalities. NELL1 also interacts with proteins such as RUNX2 which further contribute to the pathogenesis of these skeletal disorders.
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产品实验方案

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靶点信息

Plays a role in the control of cell growth and differentiation. Promotes osteoblast cell differentiation and terminal mineralization.
See full target information NELL1
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搭配使用产品

Primary Antibodies

AB197315

Anti-NELL1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELL1 antibody (AB197315)

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Proteins & Peptides

AB116955

Recombinant Human NELL1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human NELL1 protein (GST tag N-Terminus) (AB116955)

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