JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB288171

人NEFL ELISA试剂盒

Human NEFL ELISA Kit

5

(1 Review)

|

(1 Publication)

Human NEFL ELISA Kit is a single-wash 90-min Simplestep used to quantify Human NEFL with a sensitivity of 30.785 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

3 Images
ELISA - Human NEFL ELISA Kit (AB288171)
  • ELISA

Supplier Data

ELISA - Human NEFL ELISA Kit (AB288171)
Sandwich ELISA - Human NEFL ELISA Kit (AB288171)
  • sELISA

Supplier Data

Sandwich ELISA - Human NEFL ELISA Kit (AB288171)

Interpolated concentrations of spiked human NEFL in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Recombinant NEFL was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 100% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human NEFL ELISA Kit (AB288171)
  • sELISA

Supplier Data

Sandwich ELISA - Human NEFL ELISA Kit (AB288171)

Example of human NEFL standard curve in Sample Diluent NS. Example of human NEFL standard curve. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Heparin Plasma, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 30.785 pg/mL

范围

93.75 - 6000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

style
left-column

搭配使用产品

Assay Kits

AB233621

Mouse GFAP ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse GFAP ELISA Kit (AB233621)

  • 0
  • 0
Assay Kits

AB314846

Human S100B ELISA Kit - Extracellular

SimpleStep

Sandwich ELISA - Human S100B ELISA Kit - Extracellular (AB314846)

  • 0
  • 0
Assay Kits

AB318936

Human Tau (phospho T217) ELISA Kit - Extracellular

Recombinant|SimpleStep

Sandwich ELISA - Human Tau (phospho T217) ELISA Kit - Extracellular (AB318936)

  • 5
  • 1
Assay Kits

AB272199

Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein)

Recombinant|SimpleStep

Sandwich ELISA - Human Neurofilament H ELISA Kit (200 kDa Neurofilament protein) (AB272199)

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Human NEFL ELISA Kit ab288171 is a rapid single-wash 90-min Sandwich ELISA to measure Human NEFL in EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 30.785 pg/mL.

How the assay works

Human NEFL SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human NEFL ELISA Kit ab288171 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-NEFL antibody [EPR23733-137] - BSA and Azide free (Capture) ab280028
- Anti-NEFL antibody [EPR23733-154] - BSA and Azide free (Detector) ab280028
style
left-column

精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.8" } ]
style
left-column

回收率

[ { "sample": "Serum", "range": "113 - 118 %", "average": "= 116" }, { "sample": "EDTA Plasma", "range": "105 - 119 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "99 - 117 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "113 - 116 %", "average": "= 115" } ]
style
left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288171-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB288171-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB288171-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB288171-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB288171-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB288171-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288171-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288171-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human NEFL Lyophilized Recombinant Protein", "number":"AB288171-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human NEFL Capture Antibody", "number":"AB288171-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"10X Human NEFL Detector Antibody", "number":"AB288171-CMP08", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NEFL also known as neurofilament light polypeptide is a component of the cytoskeleton of neurons. It is one of the subunits of neurofilaments which also include medium and heavy chains. NEFL has a molecular mass of about 61.5 kDa. The protein is predominantly expressed in neurons throughout the central and peripheral nervous systems providing structural support and regulating axon diameter.
Biological function summary

Neurofilament light polypeptide forms heteropolymers with other neurofilament proteins contributing to the assembly of stable neurofilament fibers. These fibers maintain neuronal integrity and proper conductance velocities of nerve signals. NEFL interacts closely with neurofilament medium and heavy chains playing a role in the structural framework of the neuron. This enables efficient transmission of electrical impulses along axons.

Pathways

NEFL plays a significant role in the neurofilament pathway which is critical for neuronal cytoskeleton assembly and axonal transport. This pathway involves proteins such as NEFM (neurofilament medium) and NEFH (neurofilament heavy). NEFL's involvement in maintaining axonal caliber connects it to important signaling cascades like the axon guidance and neurotransmitter release pathways enhancing neuronal communication.

Defects in NEFL can lead to neurological conditions such as Charcot-Marie-Tooth disease type 2E and amyotrophic lateral sclerosis. Mutations or disruptions in NEFL impact axonal transport and neurofilament assembly contributing to neurodegeneration. NEFL is often studied alongside proteins like peripherin which can also exhibit mutations linked to similar neuropathies underlining its importance in neural health.
style
left-column

产品实验方案

style
left-column

靶点信息

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
See full target information NEFL
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Mikrochimica acta 192:131 PubMed39904887

2025

A turn-on fluorescent immunosensor for neurodegenerative disease related neurofilament light chain protein.

Applications

Unspecified application

Species

Unspecified reactive species

Qingting Song,Hailong Zhang,Jia Kong,Man Shing Wong,Hung-Wing Li
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com