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人Myeloperoxidase ELISA试剂盒(MPO) (ab119605)

Reviews (1)Q&A (2)References (32)
Typical Standard Curve

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 312 pg/ml - 20000 pg/ml
    • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Saliva, Serum, Tissue Homogenate, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    概述

    • 产品名称

      人Myeloperoxidase ELISA试剂盒(MPO)
      参阅全部 Myeloperoxidase 试剂盒

    • 检测方法

      Colorimetric

    • 样品类型

      Cell culture supernatant, Saliva, Urine, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate

    • 检测类型

      Sandwich (quantitative)

    • 灵敏度

      < 10 pg/ml

    • 范围

      312 pg/ml - 20000 pg/ml

    • 实验步骤

      Multiple steps standard assay

    • 种属反应性

      与反应: Human

    • 产品概述

      Abcam’s Human Myeloperoxidase (MPO) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Myeloperoxidase in cell culture supernatants, cell lysate, tissue homogenates, serum, plasma (heparin, EDTA), saliva and urine.


      A Myeloperoxidase specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Myeloperoxidase is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Myeloperoxidase amount of sample captured in plate.


      Get results in 90 minutes with Human Myeloperoxidase ELISA Kit (ab195212) from our SimpleStep ELISA® range.

    • 经测试应用

      适用于: Sandwich ELISAmore details

    • 平台

      Microplate

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab119605于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Sandwich ELISA
    Use at an assay dependent concentration.
    说明
    Sandwich ELISA
    Use at an assay dependent concentration.

    图片

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab119605.

    文献 (32)

    发表研究结果有使用 ab119605？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab119605 被引用在 32 文献中.

    • Hsu TY  et al. The abundances of LTF and SOD2 in amniotic fluid are potential biomarkers of gestational age and preterm birth. Sci Rep 13:4903 (2023). PubMed: 36966172
    • Chen L  et al. Mesenchymal stem cell-derived extracellular vesicles protect against abdominal aortic aneurysm formation by inhibiting NET-induced ferroptosis. Exp Mol Med 55:939-951 (2023). PubMed: 37121969
    • Chen J  et al. CREB1-driven CXCR4hi neutrophils promote skin inflammation in mouse models and human patients. Nat Commun 14:5894 (2023). PubMed: 37736772
    • Li H  et al. Striatal oxidative damages and neuroinflammation correlate with progression and survival of Lewy body and Alzheimer diseases. Neural Regen Res 17:867-874 (2022). PubMed: 34472487
    • Fernández-Espejo E  et al. Myeloperoxidase and Advanced Oxidation Protein Products in the Cerebrospinal Fluid in Women and Men with Parkinson's Disease. Antioxidants (Basel) 11:N/A (2022). PubMed: 35739985
    View all Publications for this product

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    MPO quantification in uterine aspirate samples

     Good Good 4/5 (Ease of Use)
    Abreviews
    abreview image
    Uterine aspirates were collected by aspiration with a Cornier Pipelle (Eurogine Ref. 03040200) in the office of the clinician or in the operating room prior to surgery and transferred to 1.5 ml microtubes. Phosphate buffer saline was added in a 1:1 (v/v) ratio and centrifuged at 2,500 rcf for 20 min in order to separate the soluble fraction (supernatant) from the solid fraction (pellet). The supernatants were kept at -80°C until use.
    The concentrations of MPO in the soluble fraction of uterine aspirates were quantified with commercially available ELISA kit (Abcam, catalog number ab119605) according to the manufacturer´s protocol. As no results are available regarding the levels of these proteins in uterine aspirates, samples were diluted using five serial dilutions: 1:2, 1:10, 1:50, 1:300, 1:1500.
    The same amount of total protein from 8 uterine aspirates was loaded in each well. All samples were assayed in duplicates using a microplate reader and values were reported as ng/mL. The mean absorbance for each set of duplicate standards and samples was calculated. The standard curve was plotted in a log10-log10 scale (see figure 1A). The CV (%) between the duplicates of the samples ranged from 4-13% (average of 7%). Concentration of MMP9 of uterine aspirates ranged from 2 to 1053 ng/ul (see figure 1B).
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 18 2016

    Question

    has the antibody in this kit been tested for cross-reactivity with other molecules such as VPO? Or can you compare the immunogen sequence for potential cross-reactivity?

    Read More
    Answer

    Thank you for contacting us. Cross-reactivity with other molecules such as VPO has not been tested, however the homology between the immunogen of the antibody in the kit and human VPO is 41%, much to low for cross-reactivity to occurs. Typically around 85% there is cross-reactivity.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Dear technical team,

    Our customer is looking for MPO ELISA kit for mouse tissue sample.
    There's only one kit in abcam's catalog.
    https://www.abcam.com/MPO-Human-ELISA-KIt-ab119605.html

    It stated Human kit but, I would like to ask if there's any possibility to work on mouse sample.
    Check this please.

    Best regards,

    Read More
    Answer

    Thank you for your enquiry.

    I can confirm that this kit (ab119605: MPO Human ELISA Kit) has been tested for cross-reactivity with human samples. It doesn't work on mouse samples.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

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