人Myeloperoxidase ELISA试剂盒(ab272101)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 33.37 pg/ml
- Range: 93.75 pg/ml - 6000 pg/ml
- Sample type: Cell culture extracts, Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Myeloperoxidase ELISA试剂盒
参阅全部 Myeloperoxidase 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 6 6% 批次间 样品 n Mean SD CV% Overall 3 6.1% -
样品类型
Cell culture supernatant, Saliva, Urine, Serum, Cell culture extracts, Cell culture media, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
33.37 pg/ml -
范围
93.75 pg/ml - 6000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 99 94% - 104% Saliva 103 94% - 118% Urine 89 85% - 95% Serum 91 88% - 94% Cell culture extracts 104 99% - 108% Cell culture media 94 86% - 98% EDTA Plasma 89 86% - 92% Cit plasma 88 86% - 91% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human Myeloperoxidase Kit (ab272101) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Myeloperoxidase protein in human serum, plasma, cell culture supernatant, urine, milk, saliva, cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Myeloperoxidase with 33.37 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Human Myeloperoxidase (MPO) is a hemoprotein expressed in the neutrophils (polymorphonuclear leukocytes) and is secreted during activation. Myeloperoxidase catalyzes the oxidation of chloride ions to hypochlorus acid, which is a potent antimicrobial agent. Myeloperoxidase pro-oxidative and pro-inflammatory properties cause an increase in MPO in multiple diseases including heart disease, myocardial infraction, and multiple sclerosis. Myeloperoxidase is a clinical marker of myocardial infraction and cardiovascular disease.
The standard protein in this product is purified from whole blood shown to be non-reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human Myeloperoxidase (MPO) Capture Antibody 1 x 600µl 10X Human Myeloperoxidase (MPO) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI2 1 x 6ml Human Myeloperoxidase (MPO) Lyophilized Purified Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (12 x 8 wells) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
疾病相关
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
序列相似性
Belongs to the peroxidase family. XPO subfamily. -
细胞定位
Lysosome. - Information by UniProt
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别名
- 84 kDa myeloperoxidase
- 89 kDa myeloperoxidase
- EC 1.11.1.7
see all -
数据库链接
- Entrez Gene: 4353 Human
- Omim: 606989 Human
- SwissProt: P05164 Human
- Unigene: 458272 Human
相关产品
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Alternative Versions
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ELISA kits
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272101于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human MPO competitor curve comparisonStandard Curve comparison between Human MPO SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Human MPO standard curve comparisonStandard Curve comparison between the original Human MPO SimpleStep ELISA kit (ab195212) and current Human MPO SimpleStep ELISA kit (ab272101).
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Example of human MPO standard curve in Sample Diluent NS.The MPO standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MPO in human serum, plasma and cell culture supernatant samples.The concentrations of MPO were measured in duplicates, interpolated from the MPO standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 25%, plasma (EDTA) 25%, and HL-60 supernatant 1: 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MPO concentration was determined to be 7.38 ng/mL in serum, 20.34 ng/mL in plasma (citrate), 13.06 ng/ml plasma (EDTA), and 1,035.38 ng/ml in HL-60 supernatant.
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Interpolated concentrations of native MPO in human HL-60 cell culture extract based on a 12.5 µg/mL extract load.The concentrations of MPO were measured in duplicate and interpolated from the MPO standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MPO concentration was determined to be 3.69 ng/ml in HL-60 cell culture extract.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MPO concentration was determined to be 20.92 ng/mL with a range of 11.65 – 38.79 ng/mL.
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Sandwich ELISA - Human Myeloperoxidase ELISA Kit (ab272101)To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab272101 被引用在 2 文献中.
- Kolar L et al. Enhanced Microvascular Adaptation to Acute Physical Stress and Reduced Oxidative Stress in Male Athletes Who Consumed Chicken Eggs Enriched with n-3 Polyunsaturated Fatty Acids and Antioxidants-Randomized Clinical Trial. Life (Basel) 13:N/A (2023). PubMed: 38004280
- Alexeev EE et al. Microbial-derived indoles inhibit neutrophil myeloperoxidase to diminish bystander tissue damage. FASEB J 35:e21552 (2021). PubMed: 33826788