AB100607

人MMP3 ELISA试剂盒

Name: 人MMP3 ELISA试剂盒 (ab100607)| Abcam中文官网
Brand: Abcam Limited
SKU: AB100607
Price: 7672 CNY
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Human MMP3 ELISA Kit

(1 Review)

(6 Publications)

Human MMP3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human MMP3 in Human in Biofluids, Cell Culture Media samples.

查看别名

STMY1, MMP3, Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP-3

1 Images

Functional Studies - Human MMP3 ELISA Kit (AB100607)

FuncS

Supplier Data

Functional Studies - Human MMP3 ELISA Kit (AB100607)

Representative Standard Curve using ab100607.

关键信息

检测方法

Colorimetric

样品类型

Plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

< 0.3 ng/mL

范围

0.41 - 100 ng/mL

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Abcam's MMP3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP3 pro and active forms in serum, plasma, and cell culture supernatants. (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended).

This assay employs an antibody specific for Human MMP3 coated on a 96- well plate. Standards and samples are pipetted into the wells and MMP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get results in 90 minutes with Human MMP3 ELISA Kit (ab189572) from our SimpleStep ELISA^® range.

Optimisation may be required with urine samples.

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回收率

[ { "sample": "Cell culture supernatant", "range": "84 - 103 %", "average": "= 94.72" }, { "sample": "Serum", "range": "83 - 103 %", "average": "= 95.39" }, { "sample": "Plasma", "range": "85 - 104 %", "average": "= 96.25" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100607-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100607-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100607-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100607-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100607-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human MMP3", "number":"AB100607-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MMP3 Microplate (12 strips x 8 wells)", "number":"AB100607-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"MMP3 Standard Human (recombinant) (lyophilized)", "number":"AB100607-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"1400X HRP-Streptavidin Concentrate", "number":"AB100607-CMP02", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MMP3 also known as stromelysin-1 is a member of the matrix metalloproteinase (MMP) family. These proteins are zinc-dependent endopeptidases. MMP3 specifically has a molecular weight of approximately 54 kDa and is responsible for degrading extracellular matrix components such as collagen fibronectin and laminin. MMP3 is expressed in various tissues including fibroblasts chondrocytes and endothelial cells. It plays a significant role in tissue remodeling wound healing and potentially aids in the breakdown of cellular matrices.

Biological function summary

MMP3 contributes to multiple physiological and pathological processes. It supports the remodeling of connective tissues and assists in normal developmental processes. MMP3 can work as part of a complex with other MMP proteins which can enhance or inhibit its activity. The balance of MMPs including MMP3 regulates the extracellular matrix turnover and is important for maintaining tissue homeostasis.

Pathways

MMP3 participates in pathways involved in tissue remodeling and inflammatory responses. It is part of cellular pathways regulated by cytokines like interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha). These pathways link MMP3 to other proteins such as MMP1 and MMP9 which perform overlapping and distinct roles in matrix degradation during inflammation and tissue repair.

MMP3 is implicated in conditions like arthritis and cardiovascular diseases. MMP3 contributes to cartilage degradation in rheumatoid arthritis and is associated with the breakdown of vascular structures in atherosclerosis. Proteins like MMP9 and MMP13 often interact with MMP3 in these pathological conditions forming a network that exacerbates tissue damage and disease progression.

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产品实验方案

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靶点信息

Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9 (PubMed : 11029580, PubMed : 1371271). Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway (PubMed : 2383557). Acts also intracellularly (PubMed : 22265821). For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage (PubMed : 21330369). In addition, plays a role in immune response and possesses antiviral activity against various viruses such as vesicular stomatitis virus, influenza A virus (H1N1) and human herpes virus 1 (PubMed : 35940311). Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities (PubMed : 35940311).

See full target information MMP3

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 30:253 PubMed39707183

2024

CXCL4 deficiency limits M4 macrophage infiltration and attenuates hyperoxia-induced lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Bingrui Yu,Siyuan Jia,Yu Chen,Rong Guan,Shuyu Chen,Wanwen Tang,Tianping Bao,Zhaofang Tian

Scientific reports 13:14622 PubMed37670049

2023

Proteomic and functional characterisation of extracellular vesicles from collagen VI deficient human fibroblasts reveals a role in cell motility.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Badosa,Mónica Roldán,Joaquín Fernández-Irigoyen,Enrique Santamaria,Cecilia Jimenez-Mallebrera

Nature communications 10:5410 PubMed31776338

2019

Thyroid hormone induces progression and invasiveness of squamous cell carcinomas by promoting a ZEB-1/E-cadherin switch.

Applications

Unspecified application

Species

Unspecified reactive species

Caterina Miro,Emery Di Cicco,Raffaele Ambrosio,Giuseppina Mancino,Daniela Di Girolamo,Annunziata Gaetana Cicatiello,Serena Sagliocchi,Annarita Nappi,Maria Angela De Stefano,Cristina Luongo,Dario Antonini,Feliciano Visconte,Silvia Varricchio,Gennaro Ilardi,Luigi Del Vecchio,Stefania Staibano,Anita Boelen,Cedric Blanpain,Caterina Missero,Domenico Salvatore,Monica Dentice

International immunopharmacology 76:105839 PubMed31520995

2019

MicroRNA-27a alleviates IL-1β-induced inflammatory response and articular cartilage degradation via TLR4/NF-κB signaling pathway in articular chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Jun Qiu,Ming-Ze Xu,Xiao-Dong Zhu,Yun-Han Ji

Artificial cells, nanomedicine, and biotechnology 47:1628-1634 PubMed31010320

2019

Antagonism of NK-1R using aprepitant suppresses inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoping Liu,Yuelan Zhu,Wei Zheng,Tangliang Qian,Haiyu Wang,Xiujuan Hou

Nutrients 11: PubMed30823635

2019

(Thunb.) Ser. Extract Attenuate UVB-Induced Photoaging through MAPK/AP-1 Inactivation in Human Skin Fibroblasts and Hairless Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hee-Soo Han,Ji-Sun Shin,Da-Bin Myung,Hye Shin Ahn,Sun Hee Lee,Hyoung Ja Kim,Kyung-Tae Lee

View all publications

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人MMP3 ELISA试剂盒

Human MMP3 ELISA Kit

Functional Studies - Human MMP3 ELISA Kit (AB100607)