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AB215083

人MMP1 ELISA试剂盒

Human MMP1 ELISA Kit

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(17 Publications)

Human MMP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MMP1 with a sensitivity of 19.64 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

查看别名

CLG, MMP1, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1

11 Images
ELISA - Human MMP1 ELISA Kit (AB215083)
  • ELISA

Supplier Data

ELISA - Human MMP1 ELISA Kit (AB215083)
Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP1 concentration was determined to be 1.15 ng/mL with a range of 0.589 – 2.17 ng/mL.

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)
  • sELISA

Unknown

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)

Example of human MMP1 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)

Interpolated concentrations of native MMP1 in SW480 cell culture supernatant samples.

The concentrations of MMP1 were measured in duplicates, interpolated from the MMP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : SW480 supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP1 concentration was determined to be 12.32 ng/mL in SW480 supernatant.

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)

Example of human MMP1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP1 concentration was determined to be 1.65 ng/mL with a range of 0.612 – 3.22 ng/mL.

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)
  • sELISA

Supplier Data

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)

Example of human MMP1 standard curve in Sample Diluent 50BS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)
  • sELISA

Lab

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)

Example of human MMP1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)
  • sELISA

Lab

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)

Interpolated concentration of native MMP1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% SW480 supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)
  • sELISA

Lab

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)

Example of human MMP1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)
  • sELISA

Lab

Sandwich ELISA - Human MMP1 ELISA Kit (AB215083)

Example of human MMP1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 19.64 pg/mL

范围

93.75 - 6000 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human MMP1 ELISA Kit ab215083 is a rapid single-wash 90-min Sandwich ELISA to measure Human MMP1 in cell culture extracts, cell culture supernatant, citrate plasma, heparin plasma, serum. This SimpleStep sensitivity is 19.64 pg/mL.

How the assay works

Human MMP1 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human MMP1 ELISA Kit ab215083 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-MMP1 antibody [EPR19769-2] - BSA and Azide free (Capture) ab242835
- Anti-MMP1 antibody [EPR19769-152] - BSA and Azide free (Detector) ab242835

MMP1 is a member of the matrix metalloproteinase family of proteins. These proteins function in the breakdown of the extracellular matrix during a variety of normal and pathological cellular process. MMP1 is an interstitial and fibroblast collagenase.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]
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回收率

[ { "sample": "Cell culture supernatant", "range": "91 - 114 %", "average": "= 103" }, { "sample": "Serum", "range": "98 - 117 %", "average": "= 106" }, { "sample": "Cell culture extracts", "range": "96 - 111 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "93 - 100 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "89 - 101 %", "average": "= 94" } ]
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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MMP1 also known as collagenase-1 is an enzyme belonging to the matrix metalloproteinase (MMP) family. It is responsible for the degradation of type I II and III collagens making it an important player in collagen turnover. MMP1 has a molecular weight of approximately 54 kDa. This enzyme is expressed in various tissues including skin lung and reproductive organs primarily during tissue remodeling and repair. Scientists often use MMP1 ELISA kits and MMP1 inhibitors to study its expression and activity levels.
Biological function summary

MMP1 plays a significant role in the modulation of the extracellular matrix. It facilitates cellular processes by breaking down structural proteins and allowing for cell migration proliferation and differentiation. Although it acts independently MMP1 works in harmony with other MMP family members to maintain extracellular matrix homeostasis and is often linked to tissue remodeling complexes. This makes the MMP1 a valuable target for researchers interested in tissue repair and fibrosis.

Pathways

Studies show MMP1 involvement in critical processes such as the inflammatory response and wound healing. In the inflammatory pathway MMP1 expression is regulated by cytokines that respond to tissue injury. Within the wound healing pathway this enzyme interacts with proteins like transforming growth factor-beta (TGF-beta) to coordinate the repair process by remodeling extracellular matrix components. This illustrates the interconnected nature of MMP1 with significant regulatory proteins.

MMP1 has significant associations with osteoarthritis and cancer metastasis. MMP1 contributes to the breakdown of cartilage in osteoarthritis highlighting its role in disease progression and joint degradation. In the context of cancer elevated MMP1 expression correlates with the metastatic potential where it assists tumor invasion by degrading the surrounding matrix. MMP1's involvement with other MMPs including MMP2 and MMP9 highlights its importance in these pathological conditions making it a therapeutic target in related research.
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产品实验方案

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靶点信息

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed : 1645757, PubMed : 2153297, PubMed : 2557822). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed : 16807369).
See full target information MMP1
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文献 (17)

Recent publications for all applications. Explore the full list and refine your search

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The Ca channel ORAI1 is a regulator of oral cancer growth and nociceptive pain.

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Extract Repairs UVB-Induced Skin Photoaging In Vitro and In Vivo: Photoprotective Effects.

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Interleukin-31 promotes fibrosis and T helper 2 polarization in systemic sclerosis.

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Ai Kuzumi,Ayumi Yoshizaki,Kazuki M Matsuda,Hirohito Kotani,Yuta Norimatsu,Maiko Fukayama,Satoshi Ebata,Takemichi Fukasawa,Asako Yoshizaki-Ogawa,Yoshihide Asano,Kyojiro Morikawa,Yutaka Kazoe,Kazuma Mawatari,Takehiko Kitamori,Shinichi Sato
View all publications
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