人MER ELISA试剂盒(ab119604)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人MER ELISA试剂盒
参阅全部 MERTK 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 16 236pg/ml 14.63 6.2% 2 16 1665pg/ml 79.92 4.8% 3 16 4937pg/ml 197.48 4% 批次间 样品 n Mean SD CV% 1 24 217pg/ml 13.67 6.2% 2 24 1701pg/ml 105.46 6.2% 3 24 4920pg/ml 221.4 4.5% -
样品类型
Cell culture supernatant, Serum, Cell Lysate, Tissue Homogenate -
检测类型
Sandwich (quantitative) -
灵敏度
< 10 pg/ml -
范围
156 pg/ml - 10000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human MER in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human MER in cell culture supernatants, cell lysates, tissue homogenates and serum.
A MER specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for MER is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human MER amount of sample captured in plate.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human MER antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human MER antibody 1 x 100µl Lyophilized recombinant Human MER standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究领域
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功能
In case of filovirus infection, seems to function as a cell entry factor. -
组织特异性
Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. -
疾病相关
Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
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别名
- c MER
- c mer proto oncogene tyrosine kinase
- c-mer
see all -
数据库链接
- Entrez Gene: 10461 Human
- Omim: 604705 Human
- SwissProt: Q12866 Human
- Unigene: 306178 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119604于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Typical Standard Curve
Representative standard curve using ab119604
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab119604 被引用在 1 文献中.
- Hou X et al. The Cardiac Microenvironment Instructs Divergent Monocyte Fates and Functions in Myocarditis. Cell Rep 28:172-189.e7 (2019). PubMed: 31269438