JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB239426

人MCP4 ELISA试剂盒

Human MCP4 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human MCP4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MCP4 with a sensitivity of 1.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

MCP4, NCC1, SCYA13, CCL13, C-C motif chemokine 13, CK-beta-10, Monocyte chemoattractant protein 4, Monocyte chemotactic protein 4, NCC-1, Small-inducible cytokine A13, MCP-4

3 Images
Sandwich ELISA - Human MCP4 ELISA Kit (AB239426)
  • sELISA

Unknown

Sandwich ELISA - Human MCP4 ELISA Kit (AB239426)

Interpolated concentrations of native MCP4 in human serum, plasma (citrate), and plasma (EDTA) samples. The concentrations of MCP4 were measured in duplicates, interpolated from the MCP4 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP4 concentration was determined to be 35.0 pg/mL in serum, 56.2 pg/mL in plasma (citrate), and 36.6 pg/mL in plasma (EDTA).

Sandwich ELISA - Human MCP4 ELISA Kit (AB239426)
  • sELISA

Unknown

Sandwich ELISA - Human MCP4 ELISA Kit (AB239426)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP4 concentration was determined to be 30.6 pg/mL with a range of 17.9 - 75.0 pg/mL.

Sandwich ELISA - Human MCP4 ELISA Kit (AB239426)
  • sELISA

Unknown

Sandwich ELISA - Human MCP4 ELISA Kit (AB239426)

Example of human MCP4 standard curve in Sample Diluent NS. The MCP4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 1.2 pg/mL

范围

3.9 - 250 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

style
left-column

搭配使用产品

Assay Kits

AB211649

Human CCL18 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CCL18 ELISA Kit (AB211649)

  • 5
  • 2
Assay Kits

AB192149

Human TSLP ELISA Kit

SimpleStep

ELISA - Human TSLP ELISA Kit (AB192149)

  • 0
  • 0
Assay Kits

AB288591

Human IL-13 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-13 ELISA Kit (AB288591)

  • 0
  • 0
Assay Kits

AB235637

Human IL-31 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Human MCP4 ELISA Kit (ab239426) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP4 protein in edta plasma, serum, cell culture supernatant, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MCP4 with 1.2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style
left-column

精确度

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" } ]
style
left-column

回收率

[ { "sample": "Serum", "range": "88 - 95 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "90 - 99 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "89 - 99 %", "average": "= 96" }, { "sample": "Cell culture media", "range": "92 - 97 %", "average": "= 95" } ]
style
left-column

规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab239426-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB239426-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB239426-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB239426-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB239426-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB239426-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB239426-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB239426-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MCP4 Capture Antibody", "number":"AB239426-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human MCP4 Lyophilized Recombinant Protein", "number":"AB239426-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MCP4 Detector Antibody", "number":"AB239426-CMP07", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MCP4 also known as CCL13 is a chemokine with a molecular mass of approximately 11 kDa. It plays a critical role in attracting immune cells to sites of inflammation. MCP4 achieves this through its interaction with G protein-coupled receptors particularly CCR2 and CCR3. The protein is primarily expressed in tissues involved in immune responses including lymph nodes spleen and bone marrow. It exhibits high expression levels at sites of allergic inflammation suggesting a specific role in these processes.
Biological function summary

MCP4 functions by mediating the recruitment of monocytes eosinophils and T lymphocytes. It is not part of a larger protein complex but rather operates as part of the chemokine signaling family. MCP4 is particularly active in coordinating responses to allergens and pathogens therefore highlighting its importance in the body's defense mechanisms. As a member of the C-C motif chemokine family it shares structural characteristics with other chemokines like CCL11 which participate in similar immune cell trafficking functions.

Pathways

MCP4 is deeply involved in inflammatory and immune pathways specifically the chemokine signaling pathway and the cytokine-cytokine receptor interaction pathway. MCP4 guides immune cells allowing them to respond effectively to cytokine signals ensuring their migration to necessary sites. Through the chemokine signaling pathway it collaborates with related chemokines like CCL5 to enhance immune responses. Its interaction with these pathways highlights its role in both routine immunity and inflammatory responses positioning it as an important player in maintaining immune homeostasis.

MCP4 correlates with conditions such as asthma and allergic rhinitis. Both of these conditions involve chronic inflammation where MCP4's role becomes more pronounced. In asthma the protein contributes to the recruitment of eosinophils and other immune cells that drive the inflammatory processes. MCP4 interactions with proteins like CCR3 are significant in exacerbating symptoms of allergic disorders making it an important target for therapeutic research aimed at modulating inflammatory responses in these diseases.
style
left-column

产品实验方案

style
left-column

靶点信息

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.
See full target information CCL13
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com