AB179886

人MCP-1 ELISA试剂盒

Human MCP-1 ELISA Kit

Recombinant
SimpleStep
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(48 Publications)

Human MCP-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MCP-1 with a sensitivity of 1.26 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1

9 Images

ELISA - Human MCP-1 ELISA Kit (AB179886)

ELISA

Supplier Data

ELISA - Human MCP-1 ELISA Kit (AB179886)

sELISA

Lab

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Interpolated concentration of native MCP1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Example of human MCP1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Interpolated concentrations of native MCP1 in human cerebrospinal fluid, urine, and cell culture supernatant samples.

The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : cerebrospinal fluid (50%)), urine 100%, and PHA stimulated PBMC supernatant 0.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 371 pg/mL in cerebrospinal fluid, 144 pg/mL in urine, and 70217 pg/mL in PHA stimulated PBMC supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native MCP1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

sELISA

Unknown

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Interpolated concentrations of native MCP1 in human serum and plasma samples.

The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 25%, plasma (heparin), 25%, and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 72 pg/mL in serum, 96 pg/mL in plasma (citrate), 101 pg/mL in plasma (heparin), and 88 pg/mL plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Serum from nine individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 113 pg/mL with a range of 58 - 247 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Example of human MCP1 standard curve in Sample Diluent NS.

The MCP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Example of human MCP1 standard curve in Sample Diluent NS. The MCP1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cerebral Spinal Fluid, Urine, Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 1.26 pg/mL

范围

4.7 - 300 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human MCP-1 ELISA Kit ab179886 is a rapid single-wash 90-min ELISA kit to measure Human MCP-1 in cerebral spinal fluid, urine, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 1.26 pg/mL.

How the assay works

Human MCP-1 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

his kit recognizes both native and recombinant human MCP1 protein in serum, plasma, urine, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.

Human MCP-1 ELISA Kit ab179886 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human MCP-1 ELISA Kit ab179886

Human MCP-1 ELISA Kit ab179886 has been used to assess inflammation in different physio-pathological contexts such as colon cancer⁽¹⁾, atherosclerosis⁽²⁾, and kidney disease⁽³⁾.

References:

⁽¹⁾Bing Liu et al. 2023, PMID: 37153045,
⁽²⁾Hongwei Dan et al. 2023, PMID: 37001814,
⁽³⁾Leting Zhou et al. 2023, PMID: 37190516

Save your precious samples

Our 384-well Human MCP-1 ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human/Monkey MCP1 Antibody Pair - BSA and Azide free ab241803

Related and recommended products

MCP-1 (CCL2) is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]

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回收率

[ { "sample": "Cell culture supernatant", "range": "97 - 99 %", "average": "= 98" }, { "sample": "Serum", "range": "94 - 101 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "90 - 97 %", "average": "= 94" }, { "sample": "Cerebral Spinal Fluid", "range": "102 - 110 %", "average": "= 105" }, { "sample": "Urine", "range": "90 - 94 %", "average": "= 92" }, { "sample": "Heparin Plasma", "range": null, "average": "= 91" }, { "sample": "Citrate plasma", "range": "91 - 95 %", "average": "= 92" } ]

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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab179886-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB179886-CMP12" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB179886-CMP10" }, { "size":"1 x 600 µL", "name":"10X Human MCP-1 Capture Antibody", "number":"AB179886-CMP07" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB179886-CMP02" }, { "size":"2 x 1 Vial", "name":"Human MCP-1 Lyophilized Recombinant Protein (ab9670)", "number":"AB179886-CMP06" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB179886-CMP09" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB179886-CMP05" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB179886-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human MCP-1 Detector Antibody", "number":"AB179886-CMP08" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB179886-CMP04" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab179886-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB179886-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB179886-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human MCP-1 Lyophilized Recombinant Protein (ab9670)", "number":"AB179886-CMP06", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB179886-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MCP-1 Capture Antibody", "number":"AB179886-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB179886-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB179886-CMP09", "productcode":"" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB179886-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human MCP-1 Detector Antibody", "number":"AB179886-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB179886-CMP11", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.

Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.

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产品实验方案

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靶点信息

Acts as a ligand for C-C chemokine receptor CCR2 (PubMed : 10529171, PubMed : 10587439, PubMed : 9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed : 10587439, PubMed : 9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed : 8195247, PubMed : 8627182, PubMed : 9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed : 8107690).

See full target information CCL2

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文献 (48)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 17:992-1004 PubMed40092084

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CNS neuroscience & therapeutics 30:e70173 PubMed39727329

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Annexin A1 Mitigates Blood-Brain Barrier Disruption in a Sepsis-Associated Encephalopathy Model by Enhancing the Expression of Occludin and Zonula Occludens-1 (ZO-1).

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Yao Li,Fang Zhou,Jiyue You,Xinran Gong

American journal of translational research 16:4967-4978 PubMed39398587

2024

Early use of SGLT2 inhibitors reduces the progression of diabetic kidney disease: a retrospective cohort study.

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Shaowei Pang,Xiaoli Li

Biotechnology and applied biochemistry 72:449-459 PubMed39318270

2024

Annexin A1 protects periodontal ligament cells against lipopolysaccharide-induced inflammatory response and cellular senescence: An implication in periodontitis.

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Unspecified application

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Shuwen Luo,Lin Zhang,Xiaoyu Li,Chunshi Tong

Journal of personalized medicine 14: PubMed39063997

2024

The Role of Pro-Inflammatory Chemokines CCL-1, 2, 4, and 5 in the Etiopathogenesis of Type 2 Diabetes Mellitus in Subjects from the Asir Region of Saudi Arabia: Correlation with Different Degrees of Obesity.

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Unspecified application

Species

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Mohammad Muzaffar Mir,Jaber Alfaifi,Shahzada Khalid Sohail,Syeda Fatima Rizvi,Md Tanwir Akhtar,Mushabab Ayed Abdullah Alghamdi,Rashid Mir,Javed Iqbal Wani,Zia Ul Sabah,Fahad A Alhumaydhi,Fahad Alremthi,AbdulElah Al Jarallah AlQahtani,Muffarah Hamid Alharthi,Masoud Ishag Elkhalifa Adam,Imadeldin Elfaki,Hany M A Sonpol

Nature communications 15:5985 PubMed39013850

2024

Aging aggravates aortic aneurysm and dissection via miR-1204-MYLK signaling axis in mice.

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Stem cells and development 33:484-495 PubMed38940748

2024

Differentiation, Metabolism, and Cardioprotective Secretory Functions of Human Cardiac Stromal Cells from Ischemic and Endocarditis Patients.

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Helen Nguyen,Chuan-Chih Hsu,Annette Meeson,Rachel Oldershaw,Gavin Richardson,Andreas Czosseck,David J Lundy

Pharmaceutics 16: PubMed38543230

2024

Reduced Production of Pro-Inflammatory and Pro-Catabolic Factors by Human Serum Metabolites Derived from a Patented Saffron Extract Intake.

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Line Pourtau,Fabien Wauquier,Line Boutin-Wittrant,David Gaudout,Benjamin Moras,Adeline Vignault,Carole Vaysse,Tristan Richard,Arnaud Courtois,Stéphanie Krisa,Véronique Roux,Nicolas Macian,Gisèle Pickering,Yohann Wittrant

View all publications

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websiteProtocolBooklet

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人MCP-1 ELISA试剂盒

Human MCP-1 ELISA Kit

ELISA - Human MCP-1 ELISA Kit (AB179886)

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

Sandwich ELISA - Human MCP-1 ELISA Kit (AB179886)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台