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AB238263

人LYVE1 ELISA试剂盒

Human LYVE1 ELISA Kit

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(2 Publications)

Human LYVE1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LYVE1 with a sensitivity of 4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

查看别名

CRSBP1, HAR, XLKD1, UNQ230/PRO263, LYVE1, Lymphatic vessel endothelial hyaluronic acid receptor 1, LYVE-1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, Hyaluronic acid receptor, CRSBP-1

3 Images
Sandwich ELISA - Human LYVE1 ELISA Kit (AB238263)
  • sELISA

Supplier Data

Sandwich ELISA - Human LYVE1 ELISA Kit (AB238263)

Interpolated concentrations of native LYVE1 in human serum and urine samples. The concentrations of LYVE1 were measured in duplicates, interpolated from the LYVE1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 500, urine 1 : 20. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LYVE1 concentration was determined to be 486 ng/mL in serum and 33 ng/mL in urine.

Sandwich ELISA - Human LYVE1 ELISA Kit (AB238263)
  • sELISA

Supplier Data

Sandwich ELISA - Human LYVE1 ELISA Kit (AB238263)

Interpolated concentrations of native LYVE1 in human plasma samples. The concentrations of LYVE1 were measured in duplicates, interpolated from the LYVE1 standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (EDTA) 1 : 500, plasma (citrate) 1 : 500, and plasma (heparin) 1 : 500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LYVE1 concentration was determined to be 375 ng/mL in plasma (EDTA), 591 ng/mL in plasma (citrate) and 372 ng/mL in plasma (Heparin).

Sandwich ELISA - Human LYVE1 ELISA Kit (AB238263)
  • sELISA

Supplier Data

Sandwich ELISA - Human LYVE1 ELISA Kit (AB238263)

Example of human LYVE1 standard curve in Sample Diluent NS. The LYVE1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

反应种属

Human

检测类型

Sandwich

Quantitative
灵敏度

= 4 pg/mL

范围

23.44 - 1500 pg/mL

检测时间

1h 30m

检测平台

Pre-coated microplate (12 x 8 well strips)

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搭配使用产品

Sample Prep & Detection Kits

AB193972

Sample Diluent NS

SimpleStep

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Assay Kits

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Recombinant|SimpleStep

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AB277718

Human TFF1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human TFF1 ELISA Kit (AB277718)

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Assay Kits

AB260051

Human THBS2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human THBS2 ELISA Kit (AB260051)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Human LYVE1 ELISA Kit (ab238263) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LYVE1 protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LYVE1 with 4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精确度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]
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回收率

[ { "sample": "Serum", "range": "103 - 115 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "105 - 117 %", "average": "= 110" }, { "sample": "Urine", "range": "101 - 108 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "106 - 115 %", "average": "= 110" }, { "sample": "Citrate plasma", "range": "107 - 117 %", "average": "= 113" } ]
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规格

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab238263-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human LYVE1 Detector Antibody", "number":"AB238263-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB238263-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB238263-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB238263-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB238263-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB238263-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB238263-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB238263-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human LYVE1 Capture Antibody", "number":"AB238263-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human LYVE1 Lyophilized Recombinant Protein", "number":"AB238263-CMP08", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein "LYVE1" also known as Lymphatic Vessel Endothelial Hyaluronan Receptor 1 has a molecular mass of about 60 kDa. This protein is located on the surface of lymphatic endothelial cells. Its expression occurs mainly within the lymphatic vessels serving as an important marker for identifying lymphatic endothelium. LYVE1 facilitates the transportation of hyaluronan an important glycosaminoglycan through lymphatic vessels playing a critical role in the maintenance of extracellular matrix and tissue fluid homeostasis.
Biological function summary

LYVE1 plays a role in lymphatic system regulation and immune cell trafficking. It forms part of a network that binds hyaluronan contributing to the regulation of cell migration and adhesion. This protein interacts with molecules like CD44 and other receptors which also participate in hyaluronan binding. LYVE1's function in maintaining the balance of hyaluronan within tissues is central affecting not only the lymphatic system but also immune responses.

Pathways

LYVE1 engages in both the hyaluronan metabolism and lymphangiogenesis pathways. Its interaction with CD44 plays a significant role in the transport and uptake of hyaluronan influencing cellular processes such as proliferation and motility. These interactions link LYVE1 to pathways controlling fluid balance and immune surveillance marking it as essential for maintaining healthy lymphatic and immune function.

LYVE1 shows a connection to lymphatic-related conditions such as lymphedema and cancer metastasis. Alterations or dysregulation of LYVE1 expression can affect lymphatic function potentially leading to the accumulation of interstitial fluid as seen in lymphedema. In cancer increased LYVE1 expression can relate to tumor metastasis where it functions alongside proteins like VEGFR-3 in promoting lymphatic vessel growth and facilitating cancer cell spread.
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产品实验方案

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靶点信息

Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes (PubMed : 10037799). Binds to pericelluar hyaluronan matrices deposited on the surface of leukocytes and facilitates cell adhesion and migration through lymphatic endothelium (PubMed : 26823460).
See full target information LYVE1
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:18148 PubMed39103428

2024

Urine biomarkers can predict prostate cancer and PI-RADS score prior to biopsy.

Applications

Unspecified application

Species

Unspecified reactive species

Blaz Pavlovic,Konstantin Bräutigam,Florence Dartiguenave,Paul Martel,Arnas Rakauskas,Valérie Cesson,Markus Veit,Pascal Oechslin,Alexander Gu,Thomas Hermanns,Karim Saba,Cédric Poyet,Andreas M Hötker,Niels J Rupp,Massimo Valerio,Laurent Derré,Daniel Eberli,Irina Banzola

ACS nano 14:651-663 PubMed31851488

2020

Multimodal Multiplexed Immunoimaging with Nanostars to Detect Multiple Immunomarkers and Monitor Response to Immunotherapies.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Chuan Ou,Xiaona Wen,Christopher A Johnson,Daniel Shae,Oscar D Ayala,Joseph A Webb,Eugene C Lin,Rossane C DeLapp,Kelli L Boyd,Ann Richmond,Anita Mahadevan-Jansen,Marjan Rafat,John T Wilson,Justin M Balko,Mohammed N Tantawy,Anna E Vilgelm,Rizia Bardhan
View all publications
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