人Luteinizing hormone ELISA试剂盒(LH) (ab178658)
Key features and details
- Sensitivity: 0.22 mU/ml
- Range: 5 mU/ml - 200 mU/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Luteinizing hormone ELISA试剂盒(LH) -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 20 <=9.21% 批次间 样品 n Mean SD CV% Serum 15 <=7.91% -
样品类型
Serum, Hep Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.22 mU/ml -
范围
5 mU/ml - 200 mU/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Luteinizing hormone (LH) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of LH in serum.
A 96-well plate has been precoated with Streptavidin. Samples, standards and the LH-HRP and Bitoin conjugate are added to the wells. Biotinylated monoclonal and enzyme labeled antibodies are added and the reactants mixed: these antibodies have high affinity and specificity and are directed against distinct and different epitopes of LH. Reaction between the various LH antibodies and native LH occurs in the microwells without competition or steric hindrance forming a soluble sandwich complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of LH in the sample and the intensity is measured at 450 nm.
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 50X Washing Solution 1 x 20ml Cover Foil 1 unit Luteinizing Hormone Coated Microplate (12 x 8 wells) 1 unit Luteinizing Hormone Control 1 x 1ml Luteinizing Hormone HRP and Biotin Conjugate 1 x 12ml Luteinizing Hormone Standard 0 - 0 mU/mL 1 x 1ml Luteinizing Hormone Standard 1 - 5 mU/mL 1 x 1ml Luteinizing Hormone Standard 2 - 25 mU/mL 1 x 1ml Luteinizing Hormone Standard 3 - 50 mU/mL 1 x 1ml Luteinizing Hormone Standard 4 - 100 mU/mL 1 x 1ml Luteinizing Hormone Standard 5 - 200 mU/mL 1 x 1ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
研究领域
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功能
Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. -
组织特异性
Pituitary gland. -
疾病相关
Defects in LHB are a cause of hypogonadism LHB-related (HGON-LHB) [MIM:152780]. Hypogonadism is characterized by infertility and pseudohermaphroditism. -
序列相似性
Belongs to the glycoprotein hormones subunit beta family. -
细胞定位
Secreted. - Information by UniProt
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别名
- CGB4
- CHORIONIC GONADOTROPIN, BETA POLYPEPTIDE 4
- hLHB
see all -
数据库链接
- Entrez Gene: 3972 Human
- Omim: 152780 Human
- SwissProt: P01229 Human
- Unigene: 154704 Human
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab178658于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. |
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab178658 被引用在 2 文献中.
- Xie J et al. Evaluation of diagnosis and prognosis with anti-mullerian hormone level in early missed abortion. Am J Transl Res 13:5350-5355 (2021). PubMed: 34150129
- Dorostghoal M et al. Overexpression of Endometrial Estrogen Receptor-Alpha in The Window of Implantation in Women with Unexplained Infertility. Int J Fertil Steril 12:37-42 (2018). PubMed: 29334205