人Lipoprotein A ELISA试剂盒

产品详情

Human Lipoprotein A ELISA Kit ab212165 is a rapid single-wash 90-min ELISA kit to measure Human Lipoprotein A in cerebral spinal fluid, urine, serum and plasma. This SimpleStep ELISA® sensitivity is 2.5 ng/mL.

How the assay works

Human Lipoprotein A SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant human Lipoprotein A protein in serum, plasmas, urine, and cerebrospinal fluid samples only. Cellular and tissue extract samples have not been tested with this kit.

Human Lipoprotein A ELISA Kit ab212165 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human Lipoprotein A ELISA Kit ab212165

Human Lipoprotein A ELISA Kit ab212165 has been used to study the effects of LDL binding in obesity after bariatric surgery⁽¹⁾and Lipoprotein A as a predictor of coronary events⁽²⁾.

References:

⁽¹⁾Olga Gurskyi et al. 2023, PMID: 37777014,
⁽²⁾Elena Aikawa et al. 2022, PMID: 35724702

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human Lipoprotein A Antibody Pair - BSA and Azide free ab241862
- Anti-Lipoprotein a antibody [EPR19731-148] - BSA and Azide free (Capture) ab242565
- Anti-Lipoprotein a antibody [EPR19731-165] - BSA and Azide free (Detector) ab242813

Related and recommended products

Human Lipoprotein A ELISA Kit ab212165 is commonly used to study cardiovascular diseases and lipid metabolism

Other ELISA kits to measure lipid metabolism are:

- Human Apoliporotein B ELISA kit ab190806

Lipoprotein A is an atherogenic lipoprotein particle formed by an assembly of LDL particles and apo(a) bound to apoB-100 component of LDL. Apolipoprotein A, the main constituent of Lipoprotein A, has serine proteinase activity and is capable of autoproteolysis. Apolipoprotein A has 4,548 amino acids, variable sizes from 200 to 700 kDa, multiple isoforms, and structural homology with plasminogen. It competes with plasminogen for its binding site, inhibiting tissue-type plasminogen activator 1 and leading to reduced fibrinolysis. High levels of Lipoprotein A in the blood is a risk factor for myocardial infarction (MI), coronary heart disease (CHD), cerebrovascular disease (CVD), atherosclerosis, thrombosis, and stroke.