人Leptin ELISA试剂盒(ab108879)
Key features and details
- Sensitivity: 45 pg/ml
- Range: 0.125 ng/ml - 32 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
-
产品名称
人Leptin ELISA试剂盒
参阅全部 Leptin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5.8% 批次间 样品 n Mean SD CV% Overall 9.9% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 45 pg/ml -
范围
0.125 ng/ml - 32 ng/ml -
回收率
= 97 %
-
检测时间
5h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Leptin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Leptin concentrations in Human cell culture supernatants, serum and plasma.
A Leptin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Leptin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Leptin captured in plate.
Get results in 90 minutes with Human Leptin ELISA Kit (ab179884) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
-
经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Leptin Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Leptin Microplate (12 x 8 well strips) 1 unit Leptin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
-
功能
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
疾病相关
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
序列相似性
Belongs to the leptin family. -
细胞定位
Secreted. - Information by UniProt
-
别名
- FLJ94114
- LEP
- LEP_HUMAN
see all -
数据库链接
- Entrez Gene: 3952 Human
- Omim: 164160 Human
- SwissProt: P41159 Human
- Unigene: 194236 Human
相关产品
-
SimpleStep ELISA kits
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108879于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
-
Typical Standard Curve
Representative Standard Curve using ab108879.
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab108879 被引用在 3 文献中.
- López-Hernández Y et al. The plasma metabolome of long COVID patients two years after infection. Sci Rep 13:12420 (2023). PubMed: 37528111
- Ataeinosrat A et al. Effects of Three Different Modes of Resistance Training on Appetite Hormones in Males With Obesity. Front Physiol 13:827335 (2022). PubMed: 35264977
- Ma JX et al. Association between obesity-associated markers and semen quality parameters and serum reproductive hormones in Chinese infertile men. Reprod Biol Endocrinol 18:95 (2020). PubMed: 32993674