人IP-10 ELISA试剂盒(ab173194)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.6 pg/ml
- Range: 12.5 pg/ml - 800 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IP-10 ELISA试剂盒
参阅全部 IP10 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% PBMC media 9 5.1% 批次间 样品 n Mean SD CV% PBMC media 3 11.1% -
样品类型
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
2.6 pg/ml -
范围
12.5 pg/ml - 800 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 103 102% - 105% Cell culture media 96 95% - 98% Hep Plasma 86 82% - 88% EDTA Plasma 101 99% - 103% Cit plasma 96 93% - 98% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human IP-10 (CXCL10) ELISA (ab173194) kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IP-10 protein in human serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human IP-10 with 1.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
说明
C-X-C motif chemokine 10 (CXCL10 or IP-10) is a small 10.8kD protein that is secreted by several cell types in response to interferon-gamma (IFNg). These cell types include monocytes, endothelial cells and fibroblasts. Upon secretion, CXCL10 is cleaved into an 8.7kD biologically active protein to function in chemotaxis for T-cells, NK cells, monocytes/macrophages and dendritic cells. In addition, CXCL10 has antitumor activity through the inhibition of bone marrow colony formation and angiogenesis. CXCL10 elicits its effects by binding to the cell surface chemokine receptor 3 (CXCR3).
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经测试应用
适用于: Sandwich ELISAmore details -
平台
Microplate
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human IP-10 Capture Antibody 1 x 600µl 10X Human IP-10 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI2 1 x 6ml Human IP-10 Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
CXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes. -
细胞定位
Secreted. - Information by UniProt
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别名
- Interferon gamma induced factor MOB1, mouse, homolog of
- Interferon gamma induced protein 10
- 10 kDa interferon gamma induced protein
see all -
数据库链接
- Entrez Gene: 3627 Human
- Omim: 147310 Human
- SwissProt: P02778 Human
- Unigene: 632586 Human
相关产品
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab173194于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Example of IP-10 beta standard curve prepared in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
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Human IP-10 ELISA kit (ab173194)Wild-type A549 control cells or IP-10 knockout A549 cells (ab266969), grown to 40% confluency, were stimulated with Recombinant Human Interferon gamma protein (ab259377) at 100 ng/ml and Recombinant human TNF alpha protein (ab259410) at 10 ng/ml or vehicle control for 16 or 32 hours.
THP-1 cells, grown to 40% confluency, were stimulated with Recombinant Human Interferon gamma protein (ab259377) at 200 ng/ml and LPS at 50 ng/mL or vehicle control for 24 hours.
The concentrations of IP-10 (CXCL10) in cell culture supernatants were measured in duplicate and interpolated from the IP-10 standard curves. IP-10 from vehicle control samples were measured in undiluted supernatants and the treated samples were diluted 200 times. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Typical ELISA dataTitration of PBMC conditioned media (+/- PHA) within the working range of the assay. Background subtracted data from triplicate measurements are plotted.
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Sandwich ELISA _ IP-10 (CXCL10) Human SimpleStep ELISA™ Kit (ab173194)This image is courtesy of an anonymous AbreviewData shows specific secrection of IP-10 (CXCL10) by human macrophages differentiated in culture for 3 days in a dose response to M1 (MCSF + IFNg).
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Sandwich ELISA - Human IP-10 ELISA Kit (ab173194)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Sandwich ELISA - Human IP-10 ELISA Kit (ab173194)To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab173194 被引用在 12 文献中.
- Ng CY et al. Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease. EMBO Rep 23:e54271 (2022). PubMed: 35403791
- Pérez-Gómez A et al. Deciphering the quality of SARS-CoV-2 specific T-cell response associated with disease severity, immune memory and heterologous response. Clin Transl Med 12:e802 (2022). PubMed: 35415890
- Xu W et al. Alterations in bile acids as metabolic signatures in the patients with human adenovirus type 7 infection. Front Med (Lausanne) 9:896409 (2022). PubMed: 36160124
- Ng CY et al. Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease. EMBO Rep 23:e55871 (2022). PubMed: 36200589
- Phan QT et al. CXCL9 Predicts the Risk of Osteoporotic Hip Fracture in a Prospective Cohort of Chinese Men-A Matched Case-Control Study. J Bone Miner Res 37:1843-1849 (2022). PubMed: 35810382