AB173194

人IP-10 ELISA试剂盒

Name: 人IP-10 ELISA试剂盒 (ab173194)| Abcam中文官网
Brand: Abcam Limited
SKU: AB173194
Price: 7845 CNY
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Human IP-10 ELISA Kit

SimpleStep
了解详情

(1 Review)

(14 Publications)

Human IP-10 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IP-10 with a sensitivity of 2.6 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 10 citations

查看别名

INP10, SCYB10, CXCL10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Small-inducible cytokine B10, Gamma-IP10, IP-10

5 Images

ELISA - Human IP-10 ELISA Kit (AB173194)

ELISA

Supplier Data

ELISA - Human IP-10 ELISA Kit (AB173194)

sELISA

AbReview

Sandwich ELISA - Human IP-10 ELISA Kit (AB173194)

Data shows specific secrection of IP-10 (CXCL10) by human macrophages differentiated in culture for 3 days in a dose response to M1 (MCSF + IFNg).

This image is courtesy of an anonymous Abreview

sELISA

Lab

Sandwich ELISA - Human IP-10 ELISA Kit (AB173194)

Wild-type A549 control cells or IP-10 knockout A549 cells (ab266969), grown to 40% confluency, were stimulated with Recombinant Human Interferon gamma protein (ab259377) at 100 ng/ml and Recombinant human TNF alpha protein (ab259410) at 10 ng/ml or vehicle control for 16 or 32 hours.

THP-1 cells, grown to 40% confluency, were stimulated with Recombinant Human Interferon gamma protein (ab259377) at 200 ng/ml and LPS at 50 ng/mL or vehicle control for 24 hours.

The concentrations of IP-10 (CXCL10) in cell culture supernatants were measured in duplicate and interpolated from the IP-10 standard curves. IP-10 from vehicle control samples were measured in undiluted supernatants and the treated samples were diluted 200 times. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human IP-10 ELISA Kit (AB173194)

Typical ELISA data.

Titration of PBMC conditioned media (+/- PHA) within the working range of the assay. Background subtracted data from triplicate measurements are plotted.

sELISA

Unknown

Sandwich ELISA - Human IP-10 ELISA Kit (AB173194)

Example of IP-10 beta standard curve prepared in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

关键信息

检测方法

Colorimetric

样品类型

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

反应种属

Human

检测类型

Sandwich (quantitative)

灵敏度

= 2.6 pg/mL

范围

12.5 - 800 pg/mL

检测时间

1h 30m

检测平台

Microplate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Human IP-10 ELISA Kit ab173194 is a rapid single-wash 90-min Sandwich ELISA to measure Human IP-10 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 2.6 pg/mL.

How the assay works

Human IP-10 SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IP-10 ELISA Kit ab173194 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

C-X-C motif chemokine 10 (CXCL10 or IP-10) is a small 10.8kD protein that is secreted by several cell types in response to interferon-gamma (IFNg). These cell types include monocytes, endothelial cells and fibroblasts. Upon secretion, CXCL10 is cleaved into an 8.7kD biologically active protein to function in chemotaxis for T-cells, NK cells, monocytes/macrophages and dendritic cells. In addition, CXCL10 has antitumor activity through the inhibition of bone marrow colony formation and angiogenesis. CXCL10 elicits its effects by binding to the cell surface chemokine receptor 3 (CXCR3).

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精确度

[ { "reproducibilityType": "Inter", "sample": "PBMC media", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.1" }, { "reproducibilityType": "Intra", "sample": "PBMC media", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" } ]

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回收率

[ { "sample": "Serum", "range": "102 - 105 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "99 - 103 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "82 - 88 %", "average": "= 86" }, { "sample": "Citrate plasma", "range": "93 - 98 %", "average": "= 96" }, { "sample": "Cell culture media", "range": "95 - 98 %", "average": "= 96" } ]

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规格

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab173194-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB173194-CMP04" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB173194-CMP03" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB173194-CMP05" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB173194-CMP09" }, { "size":"20 x 1 Vial", "name":"Human IP-10 Lyophilized Recombinant Protein", "number":"AB173194-CMP02" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB173194-CMP10" }, { "size":"10 x 600 µL", "name":"10X Human IP-10 Capture Antibody", "number":"AB173194-CMP07" }, { "size":"10 x 600 µL", "name":"10X Human IP-10 Detector Antibody", "number":"AB173194-CMP08" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB173194-CMP06" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB173194-CMP11" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab173194-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB173194-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB173194-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB173194-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB173194-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IP-10 Capture Antibody", "number":"AB173194-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IP-10 Lyophilized Recombinant Protein", "number":"AB173194-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB173194-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB173194-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IP-10 Detector Antibody", "number":"AB173194-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB173194-CMP06", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

IP10 also known as CXCL10 is a small cytokine belonging to the CXC chemokine family. It has a molecular mass of approximately 8.7 kDa. IP10 is secreted by several cell types such as monocytes endothelial cells and fibroblasts in response to interferon-gamma (IFN-γ). This protein is involved in immune responses and exhibits various roles especially in chemoattracting cells. Researchers often measure IP10 concentrations using ELISA kits such as the IP-10 ELISA to study its expression levels in different biological contexts.

Biological function summary

IP10 plays a role in modulating the activities of immune cells. It attracts T cells eosinophils monocytes and natural killer (NK) cells by binding to the CXCR3 receptor. IP10 is not part of a larger complex but interacts with other cytokines to influence cell migration and the immune response. High levels of IP10 can reflect strong immune activation which is why it is often measured in inflammatory conditions using standard assays like the IP-10 ELISA kits.

Pathways

The role of IP10 lies within the Th1-type immune response pathway. In this pathway IP10 works alongside other chemokines to recruit and activate immune cells to sites of inflammation or infection. It synergizes with IFN-γ to propagate immune signals. IP10 is also linked with the CXCR3 receptor which plays a critical role in these pathways providing a connection to other proteins such as CXCL9 and CXCL11 which have similar functions in cell-mediated immunity.

IP10 is associated with conditions like multiple sclerosis and rheumatoid arthritis. Elevated IP10 levels often correlate with disease activity in these disorders making it a potential biomarker for disease progression. The protein interacts with other inflammatory mediators such as TNF-α in regulating immune activity within these disease contexts. IP10's involvement in recruiting immune cells contributes to the pathogenic inflammation observed in these conditions.

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产品实验方案

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靶点信息

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (PubMed : 11157474, PubMed : 22652417, PubMed : 7540647). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (By similarity). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization (PubMed : 12750173, PubMed : 19151743). In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (PubMed : 12663757, PubMed : 12750173). Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (By similarity).

See full target information CXCL10

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1374447 PubMed39559356

2024

IP-10 acts early in CV-A16 infection to induce BBB destruction and promote virus entry into the CNS by increasing TNF-α expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yajie Hu,Yunguang Hu,Anguo Yin,Yaming Lv,Jiang Li,Jingyuan Fan,Baojiang Qian,Jie Song,Yunhui Zhang

Frontiers in immunology 15:1332425 PubMed38655258

2024

Effect of high-dose supplementation on hospitalized adults with COVID-19: a randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Reza Aghasadeghi,Mohammad Ali Zaheri Birgani,Saeedreza Jamalimoghadamsiyahkali,Hadiseh Hosamirudsari,Ali Moradi,Majid Jafari-Sabet,Nooshin Sadigh,Pooneh Rahimi,Rezvan Tavakoli,Mojtaba Hamidi-Fard,Golnaz Bahramali,Zohal Parmoon,Sina Arjmand Hashjin,Ghasem Mirzajani,Reza Kouhkheil,Somayeh Roshangaran,Samineh Khalaf,Mohammad Khademi Nadoushan,Ghazaleh Gholamiyan Yousef Abad,Nima Shahryarpour,Mohammad Izadi,Abolfazl Zendedel,Shayesteh Jahanfar,Omid Dadras,SeyedAhmad SeyedAlinaghi,Daniel Hackett

EMBO reports 23:e55871 PubMed36200589

2022

Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yi Ng,Khang Leng Lee,Mark Dhinesh Muthiah,Kan Xing Wu,Florence Wen Jing Chioh,Konstanze Tan,Gwyneth Shook Ting Soon,Asim Shabbir,Wai Mun Loo,Zun Siong Low,Qingfeng Chen,Walter Wahli,Nguan Soon Tan,Huck Hui Ng,Yock Young Dan,Christine Cheung

Frontiers in medicine 9:896409 PubMed36160124

2022

Alterations in bile acids as metabolic signatures in the patients with human adenovirus type 7 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Xu,Juan Du,Ting-Ting Wei,Lin-Yi Chen,Xin-Xin Yang,Tu Bo,Han-Yu Liu,Ming-Zhu Xie,Tian-Shuo Zhao,Jun-Lian Yang,Fuqiang Cui,Wei-Wei Chen,Qing-Bin Lu

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 37:1843-1849 PubMed35810382

2022

CXCL9 Predicts the Risk of Osteoporotic Hip Fracture in a Prospective Cohort of Chinese Men-A Matched Case-Control Study.

Applications

Unspecified application

Species

Unspecified reactive species

Quang Tien Phan,Kevin Yiqiang Chua,Aizhen Jin,Christoph Winkler,Woon-Puay Koh

Clinical and translational medicine 12:e802 PubMed35415890

2022

Deciphering the quality of SARS-CoV-2 specific T-cell response associated with disease severity, immune memory and heterologous response.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto Pérez-Gómez,Carmen Gasca-Capote,Joana Vitallé,Francisco J Ostos,Ana Serna-Gallego,María Trujillo-Rodríguez,Esperanza Muñoz-Muela,Teresa Giráldez-Pérez,Julia Praena-Segovia,María D Navarro-Amuedo,María Paniagua-García,Manuel García-Gutiérrez,Manuela Aguilar-Guisado,Inmaculada Rivas-Jeremías,María Reyes Jiménez-León,Sara Bachiller,Alberto Fernández-Villar,Alexandre Pérez-González,Alicia Gutiérrez-Valencia,Mohammed Rafii-El-Idrissi Benhnia,Daniela Weiskopf,Alessandro Sette,Luis F López-Cortés,Eva Poveda,Ezequiel Ruiz-Mateos

EMBO reports 23:e54271 PubMed35403791

2022

Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yi Ng,Khang Leng Lee,Mark Dhinesh Muthiah,Kan Xing Wu,Florence Wen Jing Chioh,Konstanze Tan,Gwyneth Shook Ting Soon,Asim Shabbir,Wai Mun Loo,Zun Siong Low,Qingfeng Chen,Nguan Soon Tan,Huck Hui Ng,Yock Young Dan,Christine Cheung

Cellular & molecular immunology 18:2128-2139 PubMed34290398

2021

Dendritic cell deficiencies persist seven months after SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto Pérez-Gómez,Joana Vitallé,Carmen Gasca-Capote,Alicia Gutierrez-Valencia,María Trujillo-Rodriguez,Ana Serna-Gallego,Esperanza Muñoz-Muela,María de Los Reyes Jiménez-Leon,Mohamed Rafii-El-Idrissi Benhnia,Inmaculada Rivas-Jeremias,Cesar Sotomayor,Cristina Roca-Oporto,Nuria Espinosa,Carmen Infante-Domínguez,Juan Carlos Crespo-Rivas,Alberto Fernández-Villar,Alexandre Pérez-González,Luis Fernando López-Cortés,Eva Poveda,Ezequiel Ruiz-Mateos

eLife 10: PubMed33904395

2021

CRISPR-based functional genomics in human dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Jost,Amy N Jacobson,Jeffrey A Hussmann,Giana Cirolia,Michael A Fischbach,Jonathan S Weissman

Scientific reports 10:21360 PubMed33288772

2020

Targeting macrophage priming by polyphyllin VII triggers anti-tumor immunity via STING-governed cytotoxic T-cell infiltration in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jinglu Yu,Haibin Deng,Zhenye Xu

View all publications

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websiteProtocolBooklet

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人IP-10 ELISA试剂盒

Human IP-10 ELISA Kit

ELISA - Human IP-10 ELISA Kit (AB173194)

Sandwich ELISA - Human IP-10 ELISA Kit (AB173194)

Sandwich ELISA - Human IP-10 ELISA Kit (AB173194)

Sandwich ELISA - Human IP-10 ELISA Kit (AB173194)

Sandwich ELISA - Human IP-10 ELISA Kit (AB173194)

关键信息

检测方法

样品类型

反应种属

检测类型

灵敏度

范围

检测时间

检测平台